Restriction map for
pF1KB5183
(Linear) (Six-base) MAPSORT of: KIBB5183.seq Check: 5734 from: 1 to: 1302
KIBB5183.orf
With 182 enzymes: SgfI *
April 24, 2009 13:04 ..
AccI GT'mk_AC
Cuts at: 0 563 1302
Size: 563 739
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 208 1073 1302
Size: 208 865 229
Fragments arranged by size:
865 229 208
AhdI GACnn_n'nnGTC
Cuts at: 0 783 1302
Size: 783 519
AleI CACnn'nnGTG
Cuts at: 0 616 1302
Size: 616 686
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1033 1302
Size: 1033 269
ApaI G_GGCC'C
Cuts at: 0 24 1302
Size: 24 1278
ApaLI G'TGCA_C
Cuts at: 0 1164 1302
Size: 1164 138
AvaI C'yCGr_G
Cuts at: 0 23 1302
Size: 23 1279
BamHI G'GATC_C
Cuts at: 0 935 1302
Size: 935 367
BanI G'GyrC_C
Cuts at: 0 1186 1302
Size: 1186 116
BanII G_rGCy'C
Cuts at: 0 24 1302
Size: 24 1278
BbvCI CC'TCA_GC
Cuts at: 0 145 1302
Size: 145 1157
BclI T'GATC_A
Cuts at: 0 455 1302
Size: 455 847
BglII A'GATC_T
Cuts at: 0 14 1302
Size: 14 1288
BlpI GC'TnA_GC
Cuts at: 0 371 404 1302
Size: 371 33 898
Fragments arranged by size:
898 371 33
Bme1580I G_kGCm'C
Cuts at: 0 24 107 834 1168 1191 1302
Size: 24 83 727 334 23 111
Fragments arranged by size:
727 334 111 83 24 23
BmrI ACTGGGnnnn_n'
Cuts at: 0 101 508 824 1286 1302
Size: 101 407 316 462 16
Fragments arranged by size:
462 407 316 101 16
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 160 304 673 1302
Size: 160 144 369 629
Fragments arranged by size:
629 369 160 144
Bpu10I CC'TnA_GC
Cuts at: 0 145 237 1116 1302
Size: 145 92 879 186
Fragments arranged by size:
879 186 145 92
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 559 1009 1302
Size: 559 450 293
Fragments arranged by size:
559 450 293
BsaI GGTCTCn'nnnn_
Cuts at: 0 247 873 1302
Size: 247 626 429
Fragments arranged by size:
626 429 247
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 193 223 1302
Size: 193 30 1079
Fragments arranged by size:
1079 193 30
BseYI C'CCAG_C
Cuts at: 0 825 1091 1248 1302
Size: 825 266 157 54
Fragments arranged by size:
825 266 157 54
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 575 1302
Size: 575 727
BsiHKAI G_wGCw'C
Cuts at: 0 1168 1302
Size: 1168 134
Bsp1286I G_dGCh'C
Cuts at: 0 24 107 834 1168 1191 1302
Size: 24 83 727 334 23 111
Fragments arranged by size:
727 334 111 83 24 23
BspHI T'CATG_A
Cuts at: 0 503 1229 1302
Size: 503 726 73
Fragments arranged by size:
726 503 73
BsrBI CCG'CTC
Cuts at: 0 375 987 1302
Size: 375 612 315
Fragments arranged by size:
612 375 315
BsrDI GCAATG_nn'
Cuts at: 0 398 1139 1302
Size: 398 741 163
Fragments arranged by size:
741 398 163
BsrFI r'CCGG_y
Cuts at: 0 441 1302
Size: 441 861
BstXI CCAn_nnnn'nTGG
Cuts at: 0 618 651 1087 1302
Size: 618 33 436 215
Fragments arranged by size:
618 436 215 33
BstYI r'GATC_y
Cuts at: 0 14 29 66 935 1302
Size: 14 15 37 869 367
Fragments arranged by size:
869 367 37 15 14
BtgI C'CryG_G
Cuts at: 0 42 1302
Size: 42 1260
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 201 1096 1302
Size: 201 895 206
Fragments arranged by size:
895 206 201
BtsI GCAGTG_nn'
Cuts at: 0 107 200 998 1302
Size: 107 93 798 304
Fragments arranged by size:
798 304 107 93
EaeI y'GGCC_r
Cuts at: 0 347 410 488 1302
Size: 347 63 78 814
Fragments arranged by size:
814 347 78 63
EarI CTCTTCn'nnn_
Cuts at: 0 1238 1302
Size: 1238 64
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 160 208 304 673 1073 1302
Size: 160 48 96 369 400 229
Fragments arranged by size:
400 369 229 160 96 48
EcoO109I rG'GnC_Cy
Cuts at: 0 135 879 1302
Size: 135 744 423
Fragments arranged by size:
744 423 135
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 292 324 769 801 1302
Size: 292 32 445 32 501
Fragments arranged by size:
501 445 292 32 32
HincII GTy'rAC
Cuts at: 0 1032 1302
Size: 1032 270
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 269 1302
Size: 269 1033
MscI TGG'CCA
Cuts at: 0 490 1302
Size: 490 812
MslI CAynn'nnrTG
Cuts at: 0 616 1302
Size: 616 686
MspA1I CmG'CkG
Cuts at: 0 44 370 408 825 1302
Size: 44 326 38 417 477
Fragments arranged by size:
477 417 326 44 38
NaeI GCC'GGC
Cuts at: 0 443 1302
Size: 443 859
NgoMIV G'CCGG_C
Cuts at: 0 441 1302
Size: 441 861
PflMI CCAn_nnn'nTGG
Cuts at: 0 945 1302
Size: 945 357
PfoI T'CCnGG_A
Cuts at: 0 32 650 1302
Size: 32 618 652
Fragments arranged by size:
652 618 32
PpuMI rG'GwC_Cy
Cuts at: 0 879 1302
Size: 879 423
PspOMI G'GGCC_C
Cuts at: 0 20 1302
Size: 20 1282
PstI C_TGCA'G
Cuts at: 0 102 1302
Size: 102 1200
PvuII CAG'CTG
Cuts at: 0 370 408 825 1302
Size: 370 38 417 477
Fragments arranged by size:
477 417 370 38
SacII CC_GC'GG
Cuts at: 0 45 1302
Size: 45 1257
SapI GCTCTTCn'nnn_
Cuts at: 0 1238 1302
Size: 1238 64
SexAI A'CCwGG_T
Cuts at: 0 1148 1302
Size: 1148 154
SfcI C'TryA_G
Cuts at: 0 98 1302
Size: 98 1204
SmaI CCC'GGG
Cuts at: 0 25 1302
Size: 25 1277
SmlI C'TyrA_G
Cuts at: 0 574 1024 1302
Size: 574 450 278
Fragments arranged by size:
574 450 278
StuI AGG'CCT
Cuts at: 0 1006 1302
Size: 1006 296
StyI C'CwwG_G
Cuts at: 0 668 1302
Size: 668 634
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 325 937 1035 1302
Size: 325 612 98 267
Fragments arranged by size:
612 325 267 98
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 193 225 1302
Size: 193 32 1077
Fragments arranged by size:
1077 193 32
Tth111I GACn'n_nGTC
Cuts at: 0 847 1302
Size: 847 455
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 60 330 1180 1302
Size: 60 270 850 122
Fragments arranged by size:
850 270 122 60
XmaI C'CCGG_G
Cuts at: 0 23 1302
Size: 23 1279
Enzymes that do cut:
AccI AcuI AhdI AleI AlfI ApaI ApaLI AvaI
BamHI BanI BanII BbvCI BclI BglII BlpI Bme1580I
BmrI BpmI Bpu10I BpuEI BsaI BsaXI BseYI BsgI
BsiHKAI Bsp1286I BspHI BsrBI BsrDI BsrFI BstXI BstYI
BtgI BtgZI BtsI EaeI EarI Eco57MI EcoO109I Hin4I
HincII MmeI MscI MslI MspA1I NaeI NgoMIV PflMI
PfoI PpuMI PspOMI PstI PvuII SacII SapI SexAI
SfcI SmaI SmlI StuI StyI TaqII TstI Tth111I
XcmI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AloI AlwNI ApoI AscI AseI AsiSI AvrII BaeI
BbeI BbsI BcgI BciVI BfrBI BglI BmgBI BmtI
BplI BsaAI BsaBI BsaHI BsaWI BseRI BsiEI BsiWI
BsmI BsmBI BspEI BspMI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstZ17I Bsu36I ClaI CspCI DraI DraIII
DrdI EagI EciI EcoICRI EcoNI EcoRI EcoRV FalI
FseI FspI FspAI HaeII HindIII HpaI KasI KpnI
MfeI MluI NarI NcoI NdeI NheI NotI NruI
NsiI NspI PacI PasI PciI PmeI PmlI PpiI
PshAI PsiI PspXI PsrI PvuI RsrII SacI SalI
SanDI SbfI ScaI SfiI SfoI SgfI SgrAI SnaBI
SpeI SphI SrfI SspI SwaI TatI XbaI XhoI
XmnI ZraI