Restriction map for
pF1KB5185
(Linear) (Six-base) MAPSORT of: KIBB5185.seq Check: 3713 from: 1 to: 1194
KIBB5185.orf
With 182 enzymes: SgfI *
February 15, 2010 17:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 834 1194
Size: 834 360
AccI GT'mk_AC
Cuts at: 0 635 1194
Size: 635 559
Acc65I G'GTAC_C
Cuts at: 0 17 1194
Size: 17 1177
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 256 1153 1194
Size: 256 897 41
Fragments arranged by size:
897 256 41
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 331 1114 1194
Size: 331 783 80
Fragments arranged by size:
783 331 80
AlwNI CAG_nnn'CTG
Cuts at: 0 591 620 723 867 1194
Size: 591 29 103 144 327
Fragments arranged by size:
591 327 144 103 29
ApaI G_GGCC'C
Cuts at: 0 698 1083 1194
Size: 698 385 111
Fragments arranged by size:
698 385 111
AvaI C'yCGr_G
Cuts at: 0 780 1194
Size: 780 414
BamHI G'GATC_C
Cuts at: 0 284 1194
Size: 284 910
BanI G'GyrC_C
Cuts at: 0 17 769 841 967 1026 1194
Size: 17 752 72 126 59 168
Fragments arranged by size:
752 168 126 72 59 17
BanII G_rGCy'C
Cuts at: 0 82 698 1083 1194
Size: 82 616 385 111
Fragments arranged by size:
616 385 111 82
Bme1580I G_kGCm'C
Cuts at: 0 248 698 774 1083 1194
Size: 248 450 76 309 111
Fragments arranged by size:
450 309 248 111 76
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 616 649 652 700 1189 1194
Size: 616 33 3 48 489 5
Fragments arranged by size:
616 489 48 33 5 3
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 520 556 818 1194
Size: 520 36 262 376
Fragments arranged by size:
520 376 262 36
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 416 446 1194
Size: 416 30 748
Fragments arranged by size:
748 416 30
BseYI C'CCAG_C
Cuts at: 0 687 691 961 1194
Size: 687 4 270 233
Fragments arranged by size:
687 270 233 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 715 1194
Size: 715 479
BsiHKAI G_wGCw'C
Cuts at: 0 668 1194
Size: 668 526
Bsp1286I G_dGCh'C
Cuts at: 0 82 248 668 698 774 1083 1194
Size: 82 166 420 30 76 309 111
Fragments arranged by size:
420 309 166 111 82 76 30
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 834 1082 1194
Size: 834 248 112
Fragments arranged by size:
834 248 112
BsrBI CCG'CTC
Cuts at: 0 108 258 1194
Size: 108 150 936
Fragments arranged by size:
936 150 108
BssSI C'ACGA_G
Cuts at: 0 362 1194
Size: 362 832
BstAPI GCAn_nnn'nTGC
Cuts at: 0 769 1194
Size: 769 425
BstEII G'GTnAC_C
Cuts at: 0 204 1194
Size: 204 990
BstXI CCAn_nnnn'nTGG
Cuts at: 0 304 1167 1194
Size: 304 863 27
Fragments arranged by size:
863 304 27
BstYI r'GATC_y
Cuts at: 0 284 351 554 1194
Size: 284 67 203 640
Fragments arranged by size:
640 284 203 67
Bsu36I CC'TnA_GG
Cuts at: 0 1180 1194
Size: 1180 14
BtgI C'CryG_G
Cuts at: 0 419 797 1194
Size: 419 378 397
Fragments arranged by size:
419 397 378
BtsI GCAGTG_nn'
Cuts at: 0 659 1194
Size: 659 535
DraIII CAC_nnn'GTG
Cuts at: 0 1026 1194
Size: 1026 168
EaeI y'GGCC_r
Cuts at: 0 1061 1157 1194
Size: 1061 96 37
Fragments arranged by size:
1061 96 37
EarI CTCTTCn'nnn_
Cuts at: 0 1005 1194
Size: 1005 189
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 262 886 1194
Size: 262 624 308
Fragments arranged by size:
624 308 262
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 256 616 649 652 700 1153 1189 1194
Size: 256 360 33 3 48 453 36 5
Fragments arranged by size:
453 360 256 48 36 33 5 3
EcoNI CCTnn'n_nnAGG
Cuts at: 0 342 907 1194
Size: 342 565 287
Fragments arranged by size:
565 342 287
EcoO109I rG'GnC_Cy
Cuts at: 0 301 450 528 617 912 932 1080 1112
Size: 301 149 78 89 295 20 148 32
Cuts at: 1112 1184 1194
Size: 72 10
Fragments arranged by size:
301 295 149 148 89 78 72 32
20 10
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 111 1194
Size: 111 1083
HaeII r_GCGC'y
Cuts at: 0 28 980 1194
Size: 28 952 214
Fragments arranged by size:
952 214 28
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 336 368 416 448 1194
Size: 336 32 48 32 746
Fragments arranged by size:
746 336 48 32 32
KpnI G_GTAC'C
Cuts at: 0 21 1194
Size: 21 1173
MscI TGG'CCA
Cuts at: 0 1159 1194
Size: 1159 35
MslI CAynn'nnrTG
Cuts at: 0 499 1194
Size: 499 695
MspA1I CmG'CkG
Cuts at: 0 546 691 723 965 1194
Size: 546 145 32 242 229
Fragments arranged by size:
546 242 229 145 32
PasI CC'CwG_GG
Cuts at: 0 33 297 1107 1194
Size: 33 264 810 87
Fragments arranged by size:
810 264 87 33
PflMI CCAn_nnn'nTGG
Cuts at: 0 620 723 1194
Size: 620 103 471
Fragments arranged by size:
620 471 103
PfoI T'CCnGG_A
Cuts at: 0 986 1194
Size: 986 208
PpuMI rG'GwC_Cy
Cuts at: 0 301 450 1194
Size: 301 149 744
Fragments arranged by size:
744 301 149
PspOMI G'GGCC_C
Cuts at: 0 694 1079 1194
Size: 694 385 115
Fragments arranged by size:
694 385 115
PvuII CAG'CTG
Cuts at: 0 691 723 1194
Size: 691 32 471
Fragments arranged by size:
691 471 32
SanDI GG'GwC_CC
Cuts at: 0 450 1194
Size: 450 744
SfcI C'TryA_G
Cuts at: 0 892 998 1194
Size: 892 106 196
Fragments arranged by size:
892 196 106
SmaI CCC'GGG
Cuts at: 0 782 1194
Size: 782 412
SmlI C'TyrA_G
Cuts at: 0 535 571 833 1194
Size: 535 36 262 361
Fragments arranged by size:
535 361 262 36
StyI C'CwwG_G
Cuts at: 0 524 1194
Size: 524 670
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 25 985 1194
Size: 25 960 209
Fragments arranged by size:
960 209 25
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1010 1042 1194
Size: 1010 32 152
Fragments arranged by size:
1010 152 32
XmaI C'CCGG_G
Cuts at: 0 780 1194
Size: 780 414
Enzymes that do cut:
AarI AccI Acc65I AcuI AlfI AlwNI ApaI AvaI
BamHI BanI BanII Bme1580I BpmI BpuEI BsaXI BseYI
BsgI BsiHKAI Bsp1286I BspMI BsrBI BssSI BstAPI BstEII
BstXI BstYI Bsu36I BtgI BtsI DraIII EaeI EarI
EciI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I KpnI
MscI MslI MspA1I PasI PflMI PfoI PpuMI PspOMI
PvuII SanDI SfcI SmaI SmlI StyI TaqII TstI
XmaI
Enzymes that do not cut:
AatII AclI AfeI AflII AflIII AgeI AhdI AleI
AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI
BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI BmgBI BmrI BmtI BplI Bpu10I BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BsiEI BsiWI BsmI
BsmBI BspEI BspHI BsrDI BsrFI BsrGI BssHII BstBI
BstZ17I BtgZI ClaI CspCI DraI DrdI EagI EcoICRI
EcoRI EcoRV FseI FspI FspAI HincII HindIII HpaI
KasI MfeI MluI MmeI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI NspI PacI PciI
PmeI PmlI PpiI PshAI PsiI PspXI PsrI PstI
PvuI RsrII SacI SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI
SrfI SspI StuI SwaI TatI Tth111I XbaI XcmI
XhoI XmnI ZraI