Restriction map for
pF1KB5190
(Linear) (Six-base) MAPSORT of: KIBB5190.seq Check: 9376 from: 1 to: 1764
KIBB5190.orf
With 182 enzymes: SgfI *
April 24, 2009 13:04 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1735 1764
Size: 1735 29
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 482 821 1165 1427 1604 1696 1764
Size: 482 339 344 262 177 92 68
Fragments arranged by size:
482 344 339 262 177 92 68
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 417 1764
Size: 417 1347
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1417 1449 1764
Size: 1417 32 315
Fragments arranged by size:
1417 315 32
AlwNI CAG_nnn'CTG
Cuts at: 0 391 1764
Size: 391 1373
ApoI r'AATT_y
Cuts at: 0 516 650 1214 1764
Size: 516 134 564 550
Fragments arranged by size:
564 550 516 134
AvaI C'yCGr_G
Cuts at: 0 784 1567 1764
Size: 784 783 197
Fragments arranged by size:
784 783 197
BanI G'GyrC_C
Cuts at: 0 846 1075 1117 1723 1764
Size: 846 229 42 606 41
Fragments arranged by size:
846 606 229 42 41
BanII G_rGCy'C
Cuts at: 0 543 1764
Size: 543 1221
BbeI G_GCGC'C
Cuts at: 0 1121 1764
Size: 1121 643
BbsI GAAGACnn'nnnn_
Cuts at: 0 449 809 929 1355 1764
Size: 449 360 120 426 409
Fragments arranged by size:
449 426 409 360 120
BciVI GTATCCnnnnn_n'
Cuts at: 0 140 702 1764
Size: 140 562 1062
Fragments arranged by size:
1062 562 140
BlpI GC'TnA_GC
Cuts at: 0 405 1764
Size: 405 1359
BmgBI CAC'GTC
Cuts at: 0 529 1764
Size: 529 1235
BmrI ACTGGGnnnn_n'
Cuts at: 0 11 379 1764
Size: 11 368 1385
Fragments arranged by size:
1385 368 11
BmtI G_CTAG'C
Cuts at: 0 413 1764
Size: 413 1351
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 750 1764
Size: 750 1014
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 981 1210 1320 1764
Size: 981 229 110 444
Fragments arranged by size:
981 444 229 110
Bpu10I CC'TnA_GC
Cuts at: 0 1055 1764
Size: 1055 709
BsaHI Gr'CG_yC
Cuts at: 0 1118 1764
Size: 1118 646
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 48 229 232 379 834 1764
Size: 48 181 3 147 455 930
Fragments arranged by size:
930 455 181 147 48 3
BseYI C'CCAG_C
Cuts at: 0 226 319 532 1010 1764
Size: 226 93 213 478 754
Fragments arranged by size:
754 478 226 213 93
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 509 605 1764
Size: 509 96 1159
Fragments arranged by size:
1159 509 96
BsiEI CG_ry'CG
Cuts at: 0 43 244 1764
Size: 43 201 1520
Fragments arranged by size:
1520 201 43
BsmBI CGTCTCn'nnnn_
Cuts at: 0 846 1764
Size: 846 918
Bsp1286I G_dGCh'C
Cuts at: 0 543 1764
Size: 543 1221
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 531 1735 1764
Size: 531 1204 29
Fragments arranged by size:
1204 531 29
BsrBI CCG'CTC
Cuts at: 0 1038 1764
Size: 1038 726
BsrDI GCAATG_nn'
Cuts at: 0 1146 1481 1764
Size: 1146 335 283
Fragments arranged by size:
1146 335 283
BssSI C'ACGA_G
Cuts at: 0 1276 1764
Size: 1276 488
BstXI CCAn_nnnn'nTGG
Cuts at: 0 739 1764
Size: 739 1025
BstYI r'GATC_y
Cuts at: 0 1571 1764
Size: 1571 193
BtgI C'CryG_G
Cuts at: 0 866 1764
Size: 866 898
BtsI GCAGTG_nn'
Cuts at: 0 1257 1517 1764
Size: 1257 260 247
Fragments arranged by size:
1257 260 247
DraI TTT'AAA
Cuts at: 0 273 1764
Size: 273 1491
EaeI y'GGCC_r
Cuts at: 0 869 1526 1764
Size: 869 657 238
Fragments arranged by size:
869 657 238
EarI CTCTTCn'nnn_
Cuts at: 0 159 747 1028 1181 1184 1568 1764
Size: 159 588 281 153 3 384 196
Fragments arranged by size:
588 384 281 196 159 153 3
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 122 1764
Size: 122 1642
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 482 821 981 1165 1210 1320 1427 1604
Size: 482 339 160 184 45 110 107 177
Cuts at: 1604 1696 1764
Size: 92 68
Fragments arranged by size:
482 339 184 177 160 110 107 92
68 45
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1468 1764
Size: 1468 296
FspI TGC'GCA
Cuts at: 0 1493 1764
Size: 1493 271
HaeII r_GCGC'y
Cuts at: 0 1121 1764
Size: 1121 643
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 910 939 942 971 1180 1212 1764
Size: 910 29 3 29 209 32 552
Fragments arranged by size:
910 552 209 32 29 29 3
KasI G'GCGC_C
Cuts at: 0 1117 1764
Size: 1117 647
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 943 1764
Size: 943 821
MscI TGG'CCA
Cuts at: 0 1528 1764
Size: 1528 236
MslI CAynn'nnrTG
Cuts at: 0 737 896 1238 1444 1764
Size: 737 159 342 206 320
Fragments arranged by size:
737 342 320 206 159
MspA1I CmG'CkG
Cuts at: 0 1742 1764
Size: 1742 22
NarI GG'CG_CC
Cuts at: 0 1118 1764
Size: 1118 646
NcoI C'CATG_G
Cuts at: 0 866 1764
Size: 866 898
NheI G'CTAG_C
Cuts at: 0 409 1764
Size: 409 1355
NspI r_CATG'y
Cuts at: 0 1749 1764
Size: 1749 15
PflMI CCAn_nnn'nTGG
Cuts at: 0 1476 1764
Size: 1476 288
PstI C_TGCA'G
Cuts at: 0 396 1764
Size: 396 1368
PvuI CG_AT'CG
Cuts at: 0 43 1764
Size: 43 1721
SapI GCTCTTCn'nnn_
Cuts at: 0 1028 1181 1568 1764
Size: 1028 153 387 196
Fragments arranged by size:
1028 387 196 153
ScaI AGT'ACT
Cuts at: 0 693 1764
Size: 693 1071
SfcI C'TryA_G
Cuts at: 0 392 1629 1764
Size: 392 1237 135
Fragments arranged by size:
1237 392 135
SfoI GGC'GCC
Cuts at: 0 1119 1764
Size: 1119 645
SspI AAT'ATT
Cuts at: 0 105 1764
Size: 105 1659
StyI C'CwwG_G
Cuts at: 0 866 1219 1764
Size: 866 353 545
Fragments arranged by size:
866 545 353
TatI w'GTAC_w
Cuts at: 0 143 691 1764
Size: 143 548 1073
Fragments arranged by size:
1073 548 143
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 679 711 879 911 1764
Size: 679 32 168 32 853
Fragments arranged by size:
853 679 168 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 385 1764
Size: 385 1379
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1419 1764
Size: 1419 345
XmnI GAAnn'nnTTC
Cuts at: 0 1163 1764
Size: 1163 601
Enzymes that do cut:
AarI AcuI AlfI AloI AlwNI ApoI AvaI BanI
BanII BbeI BbsI BciVI BlpI BmgBI BmrI BmtI
BplI BpmI Bpu10I BsaHI BseRI BseYI BsgI BsiEI
BsmBI Bsp1286I BspMI BsrBI BsrDI BssSI BstXI BstYI
BtgI BtsI DraI EaeI EarI EciI Eco57MI EcoNI
FspI HaeII Hin4I KasI MmeI MscI MslI MspA1I
NarI NcoI NheI NspI PflMI PstI PvuI SapI
ScaI SfcI SfoI SspI StyI TatI TstI Tth111I
XcmI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI ApaI ApaLI AscI AseI AsiSI AvrII
BaeI BamHI BbvCI BcgI BclI BfrBI BglI BglII
Bme1580I BpuEI BsaI BsaAI BsaBI BsaWI BsaXI BsiHKAI
BsiWI BsmI BspEI BspHI BsrFI BsrGI BssHII BstAPI
BstBI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraIII
DrdI EagI EcoICRI EcoO109I EcoRI EcoRV FalI FseI
FspAI HincII HindIII HpaI KpnI MfeI MluI NaeI
NdeI NgoMIV NotI NruI NsiI PacI PasI PciI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PspXI PsrI PvuII RsrII SacI SacII SalI SanDI
SbfI SexAI SfiI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI StuI SwaI TaqII XbaI XhoI
XmaI ZraI