(Linear) (Six-base) MAPSORT of: KIBB5194.seq  Check: 4510  from: 1  to: 489



KIBB5194.orf

 With 182 enzymes: SgfI * 

                        July 18, 2007 13:37  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     236     458     489 
   Size:        236     222      31

  Fragments arranged by size: 

                 236     222      31
 
ApaLI G'TGCA_C

Cuts at:      0     183     489 
   Size:        183     306
 
ApoI r'AATT_y

Cuts at:      0     271     489 
   Size:        271     218
 
BclI T'GATC_A

Cuts at:      0     377     489 
   Size:        377     112
 
Bme1580I G_kGCm'C

Cuts at:      0     187     489 
   Size:        187     302
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     124     489 
   Size:        124     365
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     197     200     412     457     489 
   Size:        197       3     212      45      32

  Fragments arranged by size: 

                 212     197      45      32       3
 
BsiHKAI G_wGCw'C

Cuts at:      0     187     489 
   Size:        187     302
 
BsmI GAATG_Cn'

Cuts at:      0     156     489 
   Size:        156     333
 
Bsp1286I G_dGCh'C

Cuts at:      0     187     489 
   Size:        187     302
 
Bsu36I CC'TnA_GG

Cuts at:      0     395     489 
   Size:        395      94
 
EarI CTCTTCn'nnn_

Cuts at:      0     434     476     489 
   Size:        434      42      13

  Fragments arranged by size: 

                 434      42      13
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     236     458     489 
   Size:        236     222      31

  Fragments arranged by size: 

                 236     222      31
 
EcoRI G'AATT_C

Cuts at:      0     271     489 
   Size:        271     218
 
EcoRV GAT'ATC

Cuts at:      0     252     489 
   Size:        252     237
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     313     361     489 
   Size:        313      48     128

  Fragments arranged by size: 

                 313     128      48
 
HincII GTy'rAC

Cuts at:      0     285     489 
   Size:        285     204
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      71     489 
   Size:         71     418
 
MslI CAynn'nnrTG

Cuts at:      0     197     489 
   Size:        197     292
 
NspI r_CATG'y

Cuts at:      0     359     489 
   Size:        359     130
 
SspI AAT'ATT

Cuts at:      0     161     489 
   Size:        161     328
 
StyI C'CwwG_G

Cuts at:      0     288     489 
   Size:        288     201
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     202     489 
   Size:        202     287
 
XmnI GAAnn'nnTTC

Cuts at:      0     308     489 
   Size:        308     181
 

 Enzymes that do cut:

     AcuI    ApaLI     ApoI     BclI Bme1580I     BmrI    BseRI  BsiHKAI
     BsmI Bsp1286I   Bsu36I     EarI  Eco57MI    EcoRI    EcoRV     FalI
   HincII     MmeI     MslI     NspI     SspI     StyI    TaqII     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI     BbsI    BbvCI     BcgI    BciVI    BfrBI     BglI    BglII
     BlpI    BmgBI     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseYI     BsgI    BsiEI
    BsiWI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI
  BstZ17I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII
     DrdI     EaeI     EagI     EciI  EcoICRI    EcoNI EcoO109I     FseI
     FspI    FspAI    HaeII    Hin4I  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MscI   MspA1I     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     StuI     SwaI     TatI     TstI  Tth111I     XbaI
     XcmI     XhoI     XmaI     ZraI