(Linear) (Six-base) MAPSORT of: KIBB5195.seq  Check: 2925  from: 1  to: 714



KIBB5195.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:04  ..

AseI AT'TA_AT

Cuts at:      0     512     536     714 
   Size:        512      24     178

  Fragments arranged by size: 

                 512     178      24
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     177     714 
   Size:        177     537
 
BbvCI CC'TCA_GC

Cuts at:      0     595     714 
   Size:        595     119
 
BclI T'GATC_A

Cuts at:      0     248     714 
   Size:        248     466
 
Bme1580I G_kGCm'C

Cuts at:      0     105     714 
   Size:        105     609
 
Bpu10I CC'TnA_GC

Cuts at:      0     595     714 
   Size:        595     119
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      92     714 
   Size:         92     622
 
Bsp1286I G_dGCh'C

Cuts at:      0     105     714 
   Size:        105     609
 
BsrDI GCAATG_nn'

Cuts at:      0     302     714 
   Size:        302     412
 
BssSI C'ACGA_G

Cuts at:      0     679     714 
   Size:        679      35
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     304     714 
   Size:        304     410
 
BtgI C'CryG_G

Cuts at:      0      89     714 
   Size:         89     625
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     156     714 
   Size:        156     558
 
BtsI GCAGTG_nn'

Cuts at:      0      80     182     392     714 
   Size:         80     102     210     322

  Fragments arranged by size: 

                 322     210     102      80
 
EaeI y'GGCC_r

Cuts at:      0     293     714 
   Size:        293     421
 
EarI CTCTTCn'nnn_

Cuts at:      0     196     679     714 
   Size:        196     483      35

  Fragments arranged by size: 

                 483     196      35
 
EcoO109I rG'GnC_Cy

Cuts at:      0     221     714 
   Size:        221     493
 
MslI CAynn'nnrTG

Cuts at:      0     175     283     714 
   Size:        175     108     431

  Fragments arranged by size: 

                 431     175     108
 
PfoI T'CCnGG_A

Cuts at:      0     404     714 
   Size:        404     310
 
PstI C_TGCA'G

Cuts at:      0     624     714 
   Size:        624      90
 
SfcI C'TryA_G

Cuts at:      0     620     714 
   Size:        620      94
 
StyI C'CwwG_G

Cuts at:      0     630     714 
   Size:        630      84
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      82     169     714 
   Size:         82      87     545

  Fragments arranged by size: 

                 545      87      82
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      95     127     714 
   Size:         95      32     587

  Fragments arranged by size: 

                 587      95      32
 

 Enzymes that do cut:

     AseI     BbsI    BbvCI     BclI Bme1580I   Bpu10I     BsgI Bsp1286I
    BsrDI    BssSI   BstAPI     BtgI    BtgZI     BtsI     EaeI     EarI
 EcoO109I     MslI     PfoI     PstI     SfcI     StyI    TaqII     TstI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     ApoI     AscI    AsiSI     AvaI    AvrII     BaeI    BamHI
     BanI    BanII     BbeI     BcgI    BciVI    BfrBI     BglI    BglII
     BlpI    BmgBI     BmrI     BmtI     BplI     BpmI    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI    BsiEI
  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI
    BsrFI    BsrGI   BssHII    BstBI   BstEII    BstXI    BstYI  BstZ17I
   Bsu36I     ClaI    CspCI     DraI   DraIII     DrdI     EagI     EciI
  EcoICRI  Eco57MI    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     MscI   MspA1I     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI    PflMI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     SwaI     TatI  Tth111I     XbaI     XcmI
     XhoI     XmaI     XmnI     ZraI