Restriction map for
pF1KB5205
(Linear) (Six-base) MAPSORT of: KIBB5205.seq Check: 4864 from: 1 to: 1629
KIBB5205.orf
With 182 enzymes: SgfI *
February 28, 2007 11:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 309 1629
Size: 309 1320
AccI GT'mk_AC
Cuts at: 0 239 1430 1629
Size: 239 1191 199
Fragments arranged by size:
1191 239 199
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 334 976 1629
Size: 334 642 653
Fragments arranged by size:
653 642 334
AflIII A'CryG_T
Cuts at: 0 920 1629
Size: 920 709
AleI CACnn'nnGTG
Cuts at: 0 286 1629
Size: 286 1343
AlwNI CAG_nnn'CTG
Cuts at: 0 83 1296 1519 1629
Size: 83 1213 223 110
Fragments arranged by size:
1213 223 110 83
AvaI C'yCGr_G
Cuts at: 0 613 730 1629
Size: 613 117 899
Fragments arranged by size:
899 613 117
BanI G'GyrC_C
Cuts at: 0 277 1129 1629
Size: 277 852 500
Fragments arranged by size:
852 500 277
BanII G_rGCy'C
Cuts at: 0 493 612 731 1398 1629
Size: 493 119 119 667 231
Fragments arranged by size:
667 493 231 119 119
BbeI G_GCGC'C
Cuts at: 0 1133 1629
Size: 1133 496
BbsI GAAGACnn'nnnn_
Cuts at: 0 658 1629
Size: 658 971
BbvCI CC'TCA_GC
Cuts at: 0 98 263 1629
Size: 98 165 1366
Fragments arranged by size:
1366 165 98
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1432 1466 1629
Size: 1432 34 163
Fragments arranged by size:
1432 163 34
BglI GCCn_nnn'nGGC
Cuts at: 0 1522 1629
Size: 1522 107
BmrI ACTGGGnnnn_n'
Cuts at: 0 894 1629
Size: 894 735
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 872 1629
Size: 872 757
Bpu10I CC'TnA_GC
Cuts at: 0 98 263 1629
Size: 98 165 1366
Fragments arranged by size:
1366 165 98
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1254 1629
Size: 1254 375
BsaI GGTCTCn'nnnn_
Cuts at: 0 1042 1629
Size: 1042 587
BsaBI GATnn'nnATC
Cuts at: 0 586 1629
Size: 586 1043
BsaHI Gr'CG_yC
Cuts at: 0 1130 1629
Size: 1130 499
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 845 875 1629
Size: 845 30 754
Fragments arranged by size:
845 754 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 961 1162 1629
Size: 961 201 467
Fragments arranged by size:
961 467 201
BseYI C'CCAG_C
Cuts at: 0 59 386 506 667 1023 1629
Size: 59 327 120 161 356 606
Fragments arranged by size:
606 356 327 161 120 59
BsiHKAI G_wGCw'C
Cuts at: 0 493 731 1629
Size: 493 238 898
Fragments arranged by size:
898 493 238
BsmI GAATG_Cn'
Cuts at: 0 643 1008 1629
Size: 643 365 621
Fragments arranged by size:
643 621 365
Bsp1286I G_dGCh'C
Cuts at: 0 493 612 731 1398 1629
Size: 493 119 119 667 231
Fragments arranged by size:
667 493 231 119 119
BspHI T'CATG_A
Cuts at: 0 1178 1629
Size: 1178 451
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 309 1265 1629
Size: 309 956 364
Fragments arranged by size:
956 364 309
BsrBI CCG'CTC
Cuts at: 0 484 1158 1571 1629
Size: 484 674 413 58
Fragments arranged by size:
674 484 413 58
BsrDI GCAATG_nn'
Cuts at: 0 242 1448 1629
Size: 242 1206 181
Fragments arranged by size:
1206 242 181
BstXI CCAn_nnnn'nTGG
Cuts at: 0 390 850 1239 1323 1629
Size: 390 460 389 84 306
Fragments arranged by size:
460 390 389 306 84
BstYI r'GATC_y
Cuts at: 0 839 951 1629
Size: 839 112 678
Fragments arranged by size:
839 678 112
Bsu36I CC'TnA_GG
Cuts at: 0 944 1629
Size: 944 685
BtgI C'CryG_G
Cuts at: 0 126 1122 1608 1629
Size: 126 996 486 21
Fragments arranged by size:
996 486 126 21
BtsI GCAGTG_nn'
Cuts at: 0 551 1629
Size: 551 1078
DrdI GACnn_nn'nnGTC
Cuts at: 0 1158 1629
Size: 1158 471
EaeI y'GGCC_r
Cuts at: 0 893 933 1617 1629
Size: 893 40 684 12
Fragments arranged by size:
893 684 40 12
EarI CTCTTCn'nnn_
Cuts at: 0 952 1040 1629
Size: 952 88 589
Fragments arranged by size:
952 589 88
EcoICRI GAG'CTC
Cuts at: 0 491 729 1629
Size: 491 238 900
Fragments arranged by size:
900 491 238
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 334 872 976 1629
Size: 334 538 104 653
Fragments arranged by size:
653 538 334 104
EcoO109I rG'GnC_Cy
Cuts at: 0 121 1629
Size: 121 1508
HaeII r_GCGC'y
Cuts at: 0 1133 1629
Size: 1133 496
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 971 1003 1418 1450 1629
Size: 971 32 415 32 179
Fragments arranged by size:
971 415 179 32 32
HincII GTy'rAC
Cuts at: 0 399 1629
Size: 399 1230
HindIII A'AGCT_T
Cuts at: 0 139 515 1466 1629
Size: 139 376 951 163
Fragments arranged by size:
951 376 163 139
HpaI GTT'AAC
Cuts at: 0 399 1629
Size: 399 1230
KasI G'GCGC_C
Cuts at: 0 1129 1629
Size: 1129 500
MfeI C'AATT_G
Cuts at: 0 794 1629
Size: 794 835
MscI TGG'CCA
Cuts at: 0 895 1629
Size: 895 734
MslI CAynn'nnrTG
Cuts at: 0 286 1237 1629
Size: 286 951 392
Fragments arranged by size:
951 392 286
MspA1I CmG'CkG
Cuts at: 0 390 627 1296 1344 1629
Size: 390 237 669 48 285
Fragments arranged by size:
669 390 285 237 48
NarI GG'CG_CC
Cuts at: 0 1130 1629
Size: 1130 499
NspI r_CATG'y
Cuts at: 0 924 1109 1405 1629
Size: 924 185 296 224
Fragments arranged by size:
924 296 224 185
PasI CC'CwG_GG
Cuts at: 0 117 1407 1629
Size: 117 1290 222
Fragments arranged by size:
1290 222 117
PciI A'CATG_T
Cuts at: 0 920 1629
Size: 920 709
PfoI T'CCnGG_A
Cuts at: 0 1281 1629
Size: 1281 348
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1555 1587 1629
Size: 1555 32 42
Fragments arranged by size:
1555 42 32
PpuMI rG'GwC_Cy
Cuts at: 0 121 1629
Size: 121 1508
PspXI vC'TCGA_Gb
Cuts at: 0 613 730 1629
Size: 613 117 899
Fragments arranged by size:
899 613 117
PstI C_TGCA'G
Cuts at: 0 225 306 572 1629
Size: 225 81 266 1057
Fragments arranged by size:
1057 266 225 81
PvuII CAG'CTG
Cuts at: 0 390 627 1296 1344 1629
Size: 390 237 669 48 285
Fragments arranged by size:
669 390 285 237 48
SacI G_AGCT'C
Cuts at: 0 493 731 1629
Size: 493 238 898
Fragments arranged by size:
898 493 238
SanDI GG'GwC_CC
Cuts at: 0 121 1629
Size: 121 1508
ScaI AGT'ACT
Cuts at: 0 904 1629
Size: 904 725
SfcI C'TryA_G
Cuts at: 0 221 302 568 1544 1629
Size: 221 81 266 976 85
Fragments arranged by size:
976 266 221 85 81
SfoI GGC'GCC
Cuts at: 0 1131 1629
Size: 1131 498
SmlI C'TyrA_G
Cuts at: 0 613 730 1269 1629
Size: 613 117 539 360
Fragments arranged by size:
613 539 360 117
SphI G_CATG'C
Cuts at: 0 1405 1629
Size: 1405 224
StuI AGG'CCT
Cuts at: 0 1321 1629
Size: 1321 308
StyI C'CwwG_G
Cuts at: 0 48 1094 1316 1322 1629
Size: 48 1046 222 6 307
Fragments arranged by size:
1046 307 222 48 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 259 400 913 1629
Size: 259 141 513 716
Fragments arranged by size:
716 513 259 141
TatI w'GTAC_w
Cuts at: 0 902 1629
Size: 902 727
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2 34 1629
Size: 2 32 1595
Fragments arranged by size:
1595 32 2
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 888 1629
Size: 888 741
XhoI C'TCGA_G
Cuts at: 0 613 730 1629
Size: 613 117 899
Fragments arranged by size:
899 613 117
XmnI GAAnn'nnTTC
Cuts at: 0 517 655 1629
Size: 517 138 974
Fragments arranged by size:
974 517 138
Enzymes that do cut:
AarI AccI AcuI AflIII AleI AlwNI AvaI BanI
BanII BbeI BbsI BbvCI BcgI BglI BmrI BpmI
Bpu10I BpuEI BsaI BsaBI BsaHI BsaXI BseRI BseYI
BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BstXI
BstYI Bsu36I BtgI BtsI DrdI EaeI EarI EcoICRI
Eco57MI EcoO109I HaeII Hin4I HincII HindIII HpaI KasI
MfeI MscI MslI MspA1I NarI NspI PasI PciI
PfoI PpiI PpuMI PspXI PstI PvuII SacI SanDI
ScaI SfcI SfoI SmlI SphI StuI StyI TaqII
TatI TstI XcmI XhoI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AhdI AlfI
AloI ApaI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BciVI BclI BfrBI BglII BlpI Bme1580I
BmgBI BmtI BplI BsaAI BsaWI BsgI BsiEI BsiWI
BsmBI BspEI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstZ17I BtgZI ClaI CspCI DraI DraIII EagI
EciI EcoNI EcoRI EcoRV FalI FseI FspI FspAI
KpnI MluI MmeI NaeI NcoI NdeI NgoMIV NheI
NotI NruI NsiI PacI PflMI PmeI PmlI PshAI
PsiI PspOMI PsrI PvuI RsrII SacII SalI SapI
SbfI SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI
SrfI SspI SwaI Tth111I XbaI XmaI ZraI