Restriction map for
pF1KB5208
(Linear) (Six-base) MAPSORT of: KIBB5208.seq Check: 465 from: 1 to: 1107
KIBB5208.orf
With 182 enzymes: SgfI *
February 28, 2007 11:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 294 1107
Size: 294 813
Acc65I G'GTAC_C
Cuts at: 0 654 1107
Size: 654 453
AclI AA'CG_TT
Cuts at: 0 827 1107
Size: 827 280
BanI G'GyrC_C
Cuts at: 0 654 1107
Size: 654 453
BanII G_rGCy'C
Cuts at: 0 272 548 730 1107
Size: 272 276 182 377
Fragments arranged by size:
377 276 272 182
BbsI GAAGACnn'nnnn_
Cuts at: 0 229 783 1107
Size: 229 554 324
Fragments arranged by size:
554 324 229
BclI T'GATC_A
Cuts at: 0 482 1107
Size: 482 625
BsaI GGTCTCn'nnnn_
Cuts at: 0 456 1048 1057 1082 1107
Size: 456 592 9 25 25
Fragments arranged by size:
592 456 25 25 9
BsaWI w'CCGG_w
Cuts at: 0 548 1107
Size: 548 559
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1011 1041 1107
Size: 1011 30 66
Fragments arranged by size:
1011 66 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 474 720 1069 1107
Size: 474 246 349 38
Fragments arranged by size:
474 349 246 38
BseYI C'CCAG_C
Cuts at: 0 279 541 861 1107
Size: 279 262 320 246
Fragments arranged by size:
320 279 262 246
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 561 1107
Size: 561 546
BsiEI CG_ry'CG
Cuts at: 0 417 1107
Size: 417 690
BsiHKAI G_wGCw'C
Cuts at: 0 272 1107
Size: 272 835
BsmBI CGTCTCn'nnnn_
Cuts at: 0 843 1107
Size: 843 264
Bsp1286I G_dGCh'C
Cuts at: 0 272 548 730 1107
Size: 272 276 182 377
Fragments arranged by size:
377 276 272 182
BspEI T'CCGG_A
Cuts at: 0 548 1107
Size: 548 559
BspHI T'CATG_A
Cuts at: 0 353 404 530 1107
Size: 353 51 126 577
Fragments arranged by size:
577 353 126 51
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 294 1107
Size: 294 813
BsrBI CCG'CTC
Cuts at: 0 998 1017 1107
Size: 998 19 90
Fragments arranged by size:
998 90 19
BsrDI GCAATG_nn'
Cuts at: 0 132 1107
Size: 132 975
BstAPI GCAn_nnn'nTGC
Cuts at: 0 392 1107
Size: 392 715
BstEII G'GTnAC_C
Cuts at: 0 759 1107
Size: 759 348
BstXI CCAn_nnnn'nTGG
Cuts at: 0 783 1107
Size: 783 324
BtgI C'CryG_G
Cuts at: 0 488 842 1058 1107
Size: 488 354 216 49
Fragments arranged by size:
488 354 216 49
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 243 1107
Size: 243 864
EaeI y'GGCC_r
Cuts at: 0 254 449 473 589 845 1107
Size: 254 195 24 116 256 262
Fragments arranged by size:
262 256 254 195 116 24
EarI CTCTTCn'nnn_
Cuts at: 0 277 955 1107
Size: 277 678 152
Fragments arranged by size:
678 277 152
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 25 1107
Size: 25 1082
EcoICRI GAG'CTC
Cuts at: 0 270 1107
Size: 270 837
EcoNI CCTnn'n_nnAGG
Cuts at: 0 735 1107
Size: 735 372
EcoO109I rG'GnC_Cy
Cuts at: 0 1073 1107
Size: 1073 34
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 981 1107
Size: 981 126
HincII GTy'rAC
Cuts at: 0 80 1107
Size: 80 1027
KpnI G_GTAC'C
Cuts at: 0 658 1107
Size: 658 449
MscI TGG'CCA
Cuts at: 0 256 591 847 1107
Size: 256 335 256 260
Fragments arranged by size:
335 260 256 256
MslI CAynn'nnrTG
Cuts at: 0 358 493 700 766 781 1107
Size: 358 135 207 66 15 326
Fragments arranged by size:
358 326 207 135 66 15
MspA1I CmG'CkG
Cuts at: 0 575 914 1107
Size: 575 339 193
Fragments arranged by size:
575 339 193
NcoI C'CATG_G
Cuts at: 0 488 842 1107
Size: 488 354 265
Fragments arranged by size:
488 354 265
NspI r_CATG'y
Cuts at: 0 879 1107
Size: 879 228
PflMI CCAn_nnn'nTGG
Cuts at: 0 25 587 1107
Size: 25 562 520
Fragments arranged by size:
562 520 25
PpuMI rG'GwC_Cy
Cuts at: 0 1073 1107
Size: 1073 34
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 604 636 1107
Size: 604 32 471
Fragments arranged by size:
604 471 32
PvuII CAG'CTG
Cuts at: 0 575 914 1107
Size: 575 339 193
Fragments arranged by size:
575 339 193
SacI G_AGCT'C
Cuts at: 0 272 1107
Size: 272 835
SapI GCTCTTCn'nnn_
Cuts at: 0 277 955 1107
Size: 277 678 152
Fragments arranged by size:
678 277 152
StyI C'CwwG_G
Cuts at: 0 488 842 1107
Size: 488 354 265
Fragments arranged by size:
488 354 265
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 431 1107
Size: 431 676
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 707 739 1107
Size: 707 32 368
Fragments arranged by size:
707 368 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 325 345 1107
Size: 325 20 762
Fragments arranged by size:
762 325 20
XmnI GAAnn'nnTTC
Cuts at: 0 964 1107
Size: 964 143
Enzymes that do cut:
AarI Acc65I AclI BanI BanII BbsI BclI BsaI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BstAPI BstEII
BstXI BtgI BtgZI EaeI EarI EciI EcoICRI EcoNI
EcoO109I FalI HincII KpnI MscI MslI MspA1I NcoI
NspI PflMI PpuMI PsrI PvuII SacI SapI StyI
TaqII TstI XcmI XmnI
Enzymes that do not cut:
AatII AccI AcuI AfeI AflII AflIII AgeI AhdI
AleI AlfI AloI AlwNI ApaI ApaLI ApoI AscI
AseI AsiSI AvaI AvrII BaeI BamHI BbeI BbvCI
BcgI BciVI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaAI BsaBI
BsaHI BsiWI BsmI BsrFI BsrGI BssHII BssSI BstBI
BstYI BstZ17I Bsu36I BtsI ClaI CspCI DraI DraIII
DrdI EagI Eco57MI EcoRI EcoRV FseI FspI FspAI
HaeII Hin4I HindIII HpaI KasI MfeI MluI MmeI
NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI
PacI PasI PciI PfoI PmeI PmlI PpiI PshAI
PsiI PspOMI PspXI PstI PvuI RsrII SacII SalI
SanDI SbfI ScaI SexAI SfcI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StuI SwaI TatI Tth111I XbaI XhoI XmaI ZraI