Restriction map for
pF1KB5210
(Linear) (Six-base) MAPSORT of: KIBB5210.seq Check: 7390 from: 1 to: 984
KIBB5210.orf
With 182 enzymes: SgfI *
April 24, 2009 13:04 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 282 984
Size: 282 702
AccI GT'mk_AC
Cuts at: 0 80 392 984
Size: 80 312 592
Fragments arranged by size:
592 312 80
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 475 718 749 984
Size: 475 243 31 235
Fragments arranged by size:
475 243 235 31
AleI CACnn'nnGTG
Cuts at: 0 253 984
Size: 253 731
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 185 984
Size: 185 799
AlwNI CAG_nnn'CTG
Cuts at: 0 537 696 984
Size: 537 159 288
Fragments arranged by size:
537 288 159
ApaLI G'TGCA_C
Cuts at: 0 170 984
Size: 170 814
AvaI C'yCGr_G
Cuts at: 0 381 984
Size: 381 603
BanI G'GyrC_C
Cuts at: 0 70 258 307 589 984
Size: 70 188 49 282 395
Fragments arranged by size:
395 282 188 70 49
BanII G_rGCy'C
Cuts at: 0 690 803 984
Size: 690 113 181
Fragments arranged by size:
690 181 113
BbsI GAAGACnn'nnnn_
Cuts at: 0 34 682 984
Size: 34 648 302
Fragments arranged by size:
648 302 34
BglI GCCn_nnn'nGGC
Cuts at: 0 811 984
Size: 811 173
Bme1580I G_kGCm'C
Cuts at: 0 102 140 174 263 594 984
Size: 102 38 34 89 331 390
Fragments arranged by size:
390 331 102 89 38 34
BmgBI CAC'GTC
Cuts at: 0 561 984
Size: 561 423
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 29 880 984
Size: 29 851 104
Fragments arranged by size:
851 104 29
Bpu10I CC'TnA_GC
Cuts at: 0 689 984
Size: 689 295
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 70 280 931 984
Size: 70 210 651 53
Fragments arranged by size:
651 210 70 53
BsaAI yAC'GTr
Cuts at: 0 24 840 984
Size: 24 816 144
Fragments arranged by size:
816 144 24
BsaHI Gr'CG_yC
Cuts at: 0 239 984
Size: 239 745
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 451 481 984
Size: 451 30 503
Fragments arranged by size:
503 451 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 177 984
Size: 177 807
BseYI C'CCAG_C
Cuts at: 0 101 262 551 932 957 984
Size: 101 161 289 381 25 27
Fragments arranged by size:
381 289 161 101 27 25
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 4 984
Size: 4 980
BsiHKAI G_wGCw'C
Cuts at: 0 174 984
Size: 174 810
Bsp1286I G_dGCh'C
Cuts at: 0 102 140 174 263 594 690 803 984
Size: 102 38 34 89 331 96 113 181
Fragments arranged by size:
331 181 113 102 96 89 38 34
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 282 984
Size: 282 702
BsrFI r'CCGG_y
Cuts at: 0 67 984
Size: 67 917
BssSI C'ACGA_G
Cuts at: 0 892 984
Size: 892 92
BstXI CCAn_nnnn'nTGG
Cuts at: 0 255 666 984
Size: 255 411 318
Fragments arranged by size:
411 318 255
BstYI r'GATC_y
Cuts at: 0 122 788 984
Size: 122 666 196
Fragments arranged by size:
666 196 122
BtgI C'CryG_G
Cuts at: 0 575 984
Size: 575 409
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 471 555 984
Size: 471 84 429
Fragments arranged by size:
471 429 84
DrdI GACnn_nn'nnGTC
Cuts at: 0 565 984
Size: 565 419
EaeI y'GGCC_r
Cuts at: 0 65 224 356 755 821 984
Size: 65 159 132 399 66 163
Fragments arranged by size:
399 163 159 132 66 65
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 29 475 718 749 880 984
Size: 29 446 243 31 131 104
Fragments arranged by size:
446 243 131 104 31 29
EcoO109I rG'GnC_Cy
Cuts at: 0 363 510 663 693 984
Size: 363 147 153 30 291
Fragments arranged by size:
363 291 153 147 30
HaeII r_GCGC'y
Cuts at: 0 294 946 984
Size: 294 652 38
Fragments arranged by size:
652 294 38
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 879 984
Size: 879 105
MscI TGG'CCA
Cuts at: 0 358 984
Size: 358 626
MslI CAynn'nnrTG
Cuts at: 0 253 256 574 984
Size: 253 3 318 410
Fragments arranged by size:
410 318 253 3
MspA1I CmG'CkG
Cuts at: 0 826 882 936 957 984
Size: 826 56 54 21 27
Fragments arranged by size:
826 56 54 27 21
NaeI GCC'GGC
Cuts at: 0 69 984
Size: 69 915
NcoI C'CATG_G
Cuts at: 0 575 984
Size: 575 409
NdeI CA'TA_TG
Cuts at: 0 219 984
Size: 219 765
NgoMIV G'CCGG_C
Cuts at: 0 67 984
Size: 67 917
PasI CC'CwG_GG
Cuts at: 0 666 984
Size: 666 318
PmlI CAC'GTG
Cuts at: 0 24 840 984
Size: 24 816 144
Fragments arranged by size:
816 144 24
PstI C_TGCA'G
Cuts at: 0 542 984
Size: 542 442
PvuII CAG'CTG
Cuts at: 0 936 957 984
Size: 936 21 27
Fragments arranged by size:
936 27 21
SfcI C'TryA_G
Cuts at: 0 538 831 984
Size: 538 293 153
Fragments arranged by size:
538 293 153
SmaI CCC'GGG
Cuts at: 0 383 984
Size: 383 601
SmlI C'TyrA_G
Cuts at: 0 85 295 946 984
Size: 85 210 651 38
Fragments arranged by size:
651 210 85 38
StyI C'CwwG_G
Cuts at: 0 359 575 659 984
Size: 359 216 84 325
Fragments arranged by size:
359 325 216 84
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 316 984
Size: 316 668
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 451 483 984
Size: 451 32 501
Fragments arranged by size:
501 451 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 318 984
Size: 318 666
XmaI C'CCGG_G
Cuts at: 0 381 984
Size: 381 603
Enzymes that do cut:
AarI AccI AcuI AleI AlfI AlwNI ApaLI AvaI
BanI BanII BbsI BglI Bme1580I BmgBI BpmI Bpu10I
BpuEI BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI
Bsp1286I BspMI BsrFI BssSI BstXI BstYI BtgI BtgZI
DrdI EaeI Eco57MI EcoO109I HaeII MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV PasI PmlI PstI
PvuII SfcI SmaI SmlI StyI TaqII TstI XcmI
XmaI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AloI ApaI ApoI AscI AseI AsiSI AvrII BaeI
BamHI BbeI BbvCI BcgI BciVI BclI BfrBI BglII
BlpI BmrI BmtI BplI BsaI BsaBI BsaWI BsiEI
BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrDI BsrGI
BssHII BstAPI BstBI BstEII BstZ17I Bsu36I BtsI ClaI
CspCI DraI DraIII EagI EarI EciI EcoICRI EcoNI
EcoRI EcoRV FalI FseI FspI FspAI Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI NarI NheI
NotI NruI NsiI NspI PacI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI
PvuI RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI
SphI SrfI SspI StuI SwaI TatI Tth111I XbaI
XhoI XmnI ZraI