Restriction map for
pF1KB5211
(Linear) (Six-base) MAPSORT of: KIBB5211.seq Check: 6470 from: 1 to: 1134
KIBB5211.orf
With 182 enzymes: SgfI *
March 3, 2008 10:57 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 304 1134
Size: 304 830
AatII G_ACGT'C
Cuts at: 0 401 919 1134
Size: 401 518 215
Fragments arranged by size:
518 401 215
AclI AA'CG_TT
Cuts at: 0 422 1134
Size: 422 712
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 703 1130 1134
Size: 703 427 4
Fragments arranged by size:
703 427 4
AfeI AGC'GCT
Cuts at: 0 868 1134
Size: 868 266
AflIII A'CryG_T
Cuts at: 0 481 901 1134
Size: 481 420 233
Fragments arranged by size:
481 420 233
ApaI G_GGCC'C
Cuts at: 0 390 1134
Size: 390 744
ApoI r'AATT_y
Cuts at: 0 703 838 1134
Size: 703 135 296
Fragments arranged by size:
703 296 135
AvaI C'yCGr_G
Cuts at: 0 381 821 1134
Size: 381 440 313
Fragments arranged by size:
440 381 313
BamHI G'GATC_C
Cuts at: 0 278 1134
Size: 278 856
BanII G_rGCy'C
Cuts at: 0 390 822 1134
Size: 390 432 312
Fragments arranged by size:
432 390 312
BbsI GAAGACnn'nnnn_
Cuts at: 0 445 954 1134
Size: 445 509 180
Fragments arranged by size:
509 445 180
BciVI GTATCCnnnnn_n'
Cuts at: 0 586 1134
Size: 586 548
BlpI GC'TnA_GC
Cuts at: 0 107 1134
Size: 107 1027
Bme1580I G_kGCm'C
Cuts at: 0 390 1134
Size: 390 744
BmgBI CAC'GTC
Cuts at: 0 348 1134
Size: 348 786
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 504 873 1111 1134
Size: 504 369 238 23
Fragments arranged by size:
504 369 238 23
Bpu10I CC'TnA_GC
Cuts at: 0 473 1134
Size: 473 661
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 378 864 1134
Size: 378 486 270
Fragments arranged by size:
486 378 270
BsaAI yAC'GTr
Cuts at: 0 275 484 904 1134
Size: 275 209 420 230
Fragments arranged by size:
420 275 230 209
BsaHI Gr'CG_yC
Cuts at: 0 164 398 435 916 1134
Size: 164 234 37 481 218
Fragments arranged by size:
481 234 218 164 37
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 757 787 1134
Size: 757 30 347
Fragments arranged by size:
757 347 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 901 1108 1134
Size: 901 207 26
Fragments arranged by size:
901 207 26
BseYI C'CCAG_C
Cuts at: 0 368 863 933 1134
Size: 368 495 70 201
Fragments arranged by size:
495 368 201 70
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 567 1134
Size: 567 567
BsiHKAI G_wGCw'C
Cuts at: 0 859 1134
Size: 859 275
BsmI GAATG_Cn'
Cuts at: 0 740 1134
Size: 740 394
Bsp1286I G_dGCh'C
Cuts at: 0 390 822 859 1134
Size: 390 432 37 275
Fragments arranged by size:
432 390 275 37
BspHI T'CATG_A
Cuts at: 0 342 1134
Size: 342 792
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 304 340 1134
Size: 304 36 794
Fragments arranged by size:
794 304 36
BsrFI r'CCGG_y
Cuts at: 0 180 1084 1134
Size: 180 904 50
Fragments arranged by size:
904 180 50
BstYI r'GATC_y
Cuts at: 0 63 278 1134
Size: 63 215 856
Fragments arranged by size:
856 215 63
Bsu36I CC'TnA_GG
Cuts at: 0 88 1134
Size: 88 1046
BtgI C'CryG_G
Cuts at: 0 392 1134
Size: 392 742
BtsI GCAGTG_nn'
Cuts at: 0 191 1134
Size: 191 943
EaeI y'GGCC_r
Cuts at: 0 254 1134
Size: 254 880
EarI CTCTTCn'nnn_
Cuts at: 0 289 1134
Size: 289 845
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 927 1134
Size: 927 207
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 504 703 873 1111 1130 1134
Size: 504 199 170 238 19 4
Fragments arranged by size:
504 238 199 170 19 4
EcoNI CCTnn'n_nnAGG
Cuts at: 0 311 1134
Size: 311 823
EcoO109I rG'GnC_Cy
Cuts at: 0 123 386 387 1134
Size: 123 263 1 747
Fragments arranged by size:
747 263 123 1
EcoRI G'AATT_C
Cuts at: 0 838 1134
Size: 838 296
EcoRV GAT'ATC
Cuts at: 0 147 1134
Size: 147 987
HaeII r_GCGC'y
Cuts at: 0 851 870 1134
Size: 851 19 264
Fragments arranged by size:
851 264 19
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 493 525 609 641 757 789 1134
Size: 493 32 84 32 116 32 345
Fragments arranged by size:
493 345 116 84 32 32 32
HincII GTy'rAC
Cuts at: 0 116 439 1134
Size: 116 323 695
Fragments arranged by size:
695 323 116
MscI TGG'CCA
Cuts at: 0 256 1134
Size: 256 878
MslI CAynn'nnrTG
Cuts at: 0 547 1134
Size: 547 587
MspA1I CmG'CkG
Cuts at: 0 368 394 524 1134
Size: 368 26 130 610
Fragments arranged by size:
610 368 130 26
NaeI GCC'GGC
Cuts at: 0 182 1086 1134
Size: 182 904 48
Fragments arranged by size:
904 182 48
NgoMIV G'CCGG_C
Cuts at: 0 180 1084 1134
Size: 180 904 50
Fragments arranged by size:
904 180 50
PflMI CCAn_nnn'nTGG
Cuts at: 0 546 1134
Size: 546 588
PfoI T'CCnGG_A
Cuts at: 0 792 1134
Size: 792 342
PmlI CAC'GTG
Cuts at: 0 275 904 1134
Size: 275 629 230
Fragments arranged by size:
629 275 230
PpuMI rG'GwC_Cy
Cuts at: 0 123 1134
Size: 123 1011
PspOMI G'GGCC_C
Cuts at: 0 386 1134
Size: 386 748
PstI C_TGCA'G
Cuts at: 0 367 976 1134
Size: 367 609 158
Fragments arranged by size:
609 367 158
PvuII CAG'CTG
Cuts at: 0 368 1134
Size: 368 766
SacII CC_GC'GG
Cuts at: 0 395 1134
Size: 395 739
SfcI C'TryA_G
Cuts at: 0 363 972 1134
Size: 363 609 162
Fragments arranged by size:
609 363 162
SmaI CCC'GGG
Cuts at: 0 383 823 1134
Size: 383 440 311
Fragments arranged by size:
440 383 311
SmlI C'TyrA_G
Cuts at: 0 357 843 1134
Size: 357 486 291
Fragments arranged by size:
486 357 291
StyI C'CwwG_G
Cuts at: 0 307 1134
Size: 307 827
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 157 645 1134
Size: 157 488 489
Fragments arranged by size:
489 488 157
TatI w'GTAC_w
Cuts at: 0 413 807 1113 1134
Size: 413 394 306 21
Fragments arranged by size:
413 394 306 21
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 272 1134
Size: 272 862
XmaI C'CCGG_G
Cuts at: 0 381 821 1134
Size: 381 440 313
Fragments arranged by size:
440 381 313
ZraI GAC'GTC
Cuts at: 0 399 917 1134
Size: 399 518 217
Fragments arranged by size:
518 399 217
Enzymes that do cut:
AarI AatII AclI AcuI AfeI AflIII ApaI ApoI
AvaI BamHI BanII BbsI BciVI BlpI Bme1580I BmgBI
BpmI Bpu10I BpuEI BsaAI BsaHI BsaXI BseRI BseYI
BsgI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrFI BstYI
Bsu36I BtgI BtsI EaeI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI EcoRV HaeII Hin4I HincII MscI MslI
MspA1I NaeI NgoMIV PflMI PfoI PmlI PpuMI PspOMI
PstI PvuII SacII SfcI SmaI SmlI StyI TaqII
TatI XcmI XmaI ZraI
Enzymes that do not cut:
AccI Acc65I AflII AgeI AhdI AleI AlfI AloI
AlwNI ApaLI AscI AseI AsiSI AvrII BaeI BanI
BbeI BbvCI BcgI BclI BfrBI BglI BglII BmrI
BmtI BplI BsaI BsaBI BsaWI BsiEI BsiWI BsmBI
BspEI BsrBI BsrDI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstZ17I BtgZI ClaI CspCI DraI DraIII
DrdI EagI EcoICRI FalI FseI FspI FspAI HindIII
HpaI KasI KpnI MfeI MluI MmeI NarI NcoI
NdeI NheI NotI NruI NsiI NspI PacI PasI
PciI PmeI PpiI PshAI PsiI PspXI PsrI PvuI
RsrII SacI SalI SanDI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI
SspI StuI SwaI TstI Tth111I XbaI XhoI XmnI