(Linear) (Six-base) MAPSORT of: KIBB5213.seq  Check: 762  from: 1  to: 435



KIBB5213.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:04  ..

AatII G_ACGT'C

Cuts at:      0     372     435 
   Size:        372      63
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0       8      40     435 
   Size:          8      32     395

  Fragments arranged by size: 

                 395      32       8
 
ApoI r'AATT_y

Cuts at:      0     330     435 
   Size:        330     105
 
AseI AT'TA_AT

Cuts at:      0     257     435 
   Size:        257     178
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      12     435 
   Size:         12     423
 
BsaHI Gr'CG_yC

Cuts at:      0     369     435 
   Size:        369      66
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0       8      38     435 
   Size:          8      30     397

  Fragments arranged by size: 

                 397      30       8
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     218     435 
   Size:        218     217
 
BseYI C'CCAG_C

Cuts at:      0      95     435 
   Size:         95     340
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     288     435 
   Size:        288     147
 
EcoO109I rG'GnC_Cy

Cuts at:      0      99     435 
   Size:         99     336
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     370     435 
   Size:        370      65
 
HindIII A'AGCT_T

Cuts at:      0     397     435 
   Size:        397      38
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      26     435 
   Size:         26     409
 
MslI CAynn'nnrTG

Cuts at:      0     310     435 
   Size:        310     125
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0       8      40     435 
   Size:          8      32     395

  Fragments arranged by size: 

                 395      32       8
 
StyI C'CwwG_G

Cuts at:      0     160     435 
   Size:        160     275
 
TatI w'GTAC_w

Cuts at:      0     351     435 
   Size:        351      84
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     288     435 
   Size:        288     147
 
ZraI GAC'GTC

Cuts at:      0     370     435 
   Size:        370      65
 

 Enzymes that do cut:

    AatII     AloI     ApoI     AseI     BsaI    BsaHI    BsaXI    BseRI
    BseYI    BstXI EcoO109I     FalI  HindIII     MmeI     MslI     PpiI
     StyI     TatI     XcmI     ZraI

 Enzymes that do not cut: 

     AarI     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI    AlwNI     ApaI    ApaLI     AscI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI
     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII
     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I
    BpuEI    BsaAI    BsaBI    BsaWI     BsgI    BsiEI  BsiHKAI    BsiWI
     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstYI
  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI
   DraIII     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI
    EcoNI    EcoRI    EcoRV     FseI     FspI    FspAI    HaeII    Hin4I
   HincII     HpaI     KasI     KpnI     MfeI     MluI     MscI   MspA1I
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI
     PmlI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI
     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     SwaI    TaqII     TstI  Tth111I     XbaI     XhoI     XmaI     XmnI