(Linear) (Six-base) MAPSORT of: KIBB5221.seq  Check: 66  from: 1  to: 996



KIBB5221.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:57  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     687     996 
   Size:        687     309
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     652     996 
   Size:        652     344
 
ApaLI G'TGCA_C

Cuts at:      0     808     996 
   Size:        808     188
 
ApoI r'AATT_y

Cuts at:      0     352     996 
   Size:        352     644
 
BamHI G'GATC_C

Cuts at:      0     974     996 
   Size:        974      22
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     170     640     852     996 
   Size:        170     470     212     144

  Fragments arranged by size: 

                 470     212     170     144
 
BclI T'GATC_A

Cuts at:      0     722     996 
   Size:        722     274
 
Bme1580I G_kGCm'C

Cuts at:      0     294     812     996 
   Size:        294     518     184

  Fragments arranged by size: 

                 518     294     184
 
BmgBI CAC'GTC

Cuts at:      0     295     996 
   Size:        295     701
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     808     996 
   Size:        808     188
 
BsaAI yAC'GTr

Cuts at:      0     435     996 
   Size:        435     561
 
BseYI C'CCAG_C

Cuts at:      0     286     531     996 
   Size:        286     245     465

  Fragments arranged by size: 

                 465     286     245
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     625     977     996 
   Size:        625     352      19

  Fragments arranged by size: 

                 625     352      19
 
BsiHKAI G_wGCw'C

Cuts at:      0     812     996 
   Size:        812     184
 
Bsp1286I G_dGCh'C

Cuts at:      0     294     812     996 
   Size:        294     518     184

  Fragments arranged by size: 

                 518     294     184
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     687     996 
   Size:        687     309
 
BstYI r'GATC_y

Cuts at:      0     189     974     996 
   Size:        189     785      22

  Fragments arranged by size: 

                 785     189      22
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     398     433     996 
   Size:        398      35     563

  Fragments arranged by size: 

                 563     398      35
 
DraI TTT'AAA

Cuts at:      0     351     996 
   Size:        351     645
 
EarI CTCTTCn'nnn_

Cuts at:      0     929     946     996 
   Size:        929      17      50

  Fragments arranged by size: 

                 929      50      17
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     652     996 
   Size:        652     344
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     910     996 
   Size:        910      86
 
EcoO109I rG'GnC_Cy

Cuts at:      0     564     996 
   Size:        564     432
 
EcoRV GAT'ATC

Cuts at:      0     423     996 
   Size:        423     573
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      15      47     174     206     819     851     996 
   Size:         15      32     127      32     613      32     145

  Fragments arranged by size: 

                 613     145     127      32      32      32      15
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     219     996 
   Size:        219     777
 
MspA1I CmG'CkG

Cuts at:      0      21     996 
   Size:         21     975
 
PpuMI rG'GwC_Cy

Cuts at:      0     564     996 
   Size:        564     432
 
PsiI TTA'TAA

Cuts at:      0      29     996 
   Size:         29     967
 
PvuII CAG'CTG

Cuts at:      0      21     996 
   Size:         21     975
 
SfcI C'TryA_G

Cuts at:      0     764     996 
   Size:        764     232
 
StyI C'CwwG_G

Cuts at:      0     567     996 
   Size:        567     429
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     548     557     659     827     996 
   Size:        548       9     102     168     169

  Fragments arranged by size: 

                 548     169     168     102       9
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     435     467     802     834     996 
   Size:        435      32     335      32     162

  Fragments arranged by size: 

                 435     335     162      32      32
 

 Enzymes that do cut:

     AarI     AcuI    ApaLI     ApoI    BamHI     BbsI     BclI Bme1580I
    BmgBI     BmrI    BsaAI    BseYI     BsgI  BsiHKAI Bsp1286I    BspMI
    BstYI    CspCI     DraI     EarI  Eco57MI    EcoNI EcoO109I    EcoRV
    Hin4I     MmeI   MspA1I    PpuMI     PsiI    PvuII     SfcI     StyI
    TaqII     TstI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI     BanI    BanII     BbeI    BbvCI
     BcgI    BciVI    BfrBI     BglI    BglII     BlpI     BmtI     BplI
     BpmI   Bpu10I    BpuEI     BsaI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrBI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI   DraIII
     DrdI     EaeI     EagI     EciI  EcoICRI    EcoRI     FalI     FseI
     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MscI     MslI     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PshAI   PspOMI
    PspXI     PsrI     PstI     PvuI    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     SwaI     TatI  Tth111I     XbaI     XcmI     XhoI     XmaI
     XmnI     ZraI