Restriction map for
pF1KB5224
(Linear) (Six-base) MAPSORT of: KIBB5224.seq Check: 8136 from: 1 to: 723
KIBB5224.orf
With 182 enzymes: SgfI *
February 28, 2007 11:27 ..
AleI CACnn'nnGTG
Cuts at: 0 658 691 723
Size: 658 33 32
Fragments arranged by size:
658 33 32
AlwNI CAG_nnn'CTG
Cuts at: 0 364 540 723
Size: 364 176 183
Fragments arranged by size:
364 183 176
ApoI r'AATT_y
Cuts at: 0 663 723
Size: 663 60
BanI G'GyrC_C
Cuts at: 0 401 723
Size: 401 322
BbsI GAAGACnn'nnnn_
Cuts at: 0 383 580 723
Size: 383 197 143
Fragments arranged by size:
383 197 143
BglI GCCn_nnn'nGGC
Cuts at: 0 53 723
Size: 53 670
BlpI GC'TnA_GC
Cuts at: 0 702 723
Size: 702 21
BmrI ACTGGGnnnn_n'
Cuts at: 0 97 265 279 723
Size: 97 168 14 444
Fragments arranged by size:
444 168 97 14
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 78 550 723
Size: 78 472 173
Fragments arranged by size:
472 173 78
Bpu10I CC'TnA_GC
Cuts at: 0 475 723
Size: 475 248
BsaAI yAC'GTr
Cuts at: 0 693 723
Size: 693 30
BsaWI w'CCGG_w
Cuts at: 0 386 503 723
Size: 386 117 220
Fragments arranged by size:
386 220 117
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 75 511 711 723
Size: 75 436 200 12
Fragments arranged by size:
436 200 75 12
BseYI C'CCAG_C
Cuts at: 0 13 723
Size: 13 710
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 24 723
Size: 24 699
BsiEI CG_ry'CG
Cuts at: 0 493 723
Size: 493 230
BsiHKAI G_wGCw'C
Cuts at: 0 67 723
Size: 67 656
Bsp1286I G_dGCh'C
Cuts at: 0 67 723
Size: 67 656
BspEI T'CCGG_A
Cuts at: 0 386 723
Size: 386 337
BspHI T'CATG_A
Cuts at: 0 20 723
Size: 20 703
BsrFI r'CCGG_y
Cuts at: 0 52 488 723
Size: 52 436 235
Fragments arranged by size:
436 235 52
BssSI C'ACGA_G
Cuts at: 0 262 723
Size: 262 461
BstAPI GCAn_nnn'nTGC
Cuts at: 0 540 723
Size: 540 183
BstXI CCAn_nnnn'nTGG
Cuts at: 0 528 612 723
Size: 528 84 111
Fragments arranged by size:
528 111 84
Bsu36I CC'TnA_GG
Cuts at: 0 169 723
Size: 169 554
BtgI C'CryG_G
Cuts at: 0 137 723
Size: 137 586
BtsI GCAGTG_nn'
Cuts at: 0 521 723
Size: 521 202
DrdI GACnn_nn'nnGTC
Cuts at: 0 148 723
Size: 148 575
EaeI y'GGCC_r
Cuts at: 0 518 602 614 723
Size: 518 84 12 109
Fragments arranged by size:
518 109 84 12
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 78 550 723
Size: 78 472 173
Fragments arranged by size:
472 173 78
EcoNI CCTnn'n_nnAGG
Cuts at: 0 409 693 723
Size: 409 284 30
Fragments arranged by size:
409 284 30
EcoO109I rG'GnC_Cy
Cuts at: 0 513 723
Size: 513 210
HincII GTy'rAC
Cuts at: 0 153 723
Size: 153 570
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 94 473 723
Size: 94 379 250
Fragments arranged by size:
379 250 94
MscI TGG'CCA
Cuts at: 0 520 604 616 723
Size: 520 84 12 107
Fragments arranged by size:
520 107 84 12
MslI CAynn'nnrTG
Cuts at: 0 583 658 691 723
Size: 583 75 33 32
Fragments arranged by size:
583 75 33 32
MspA1I CmG'CkG
Cuts at: 0 8 537 723
Size: 8 529 186
Fragments arranged by size:
529 186 8
NaeI GCC'GGC
Cuts at: 0 54 723
Size: 54 669
NcoI C'CATG_G
Cuts at: 0 137 723
Size: 137 586
NgoMIV G'CCGG_C
Cuts at: 0 52 723
Size: 52 671
PflMI CCAn_nnn'nTGG
Cuts at: 0 138 723
Size: 138 585
PpuMI rG'GwC_Cy
Cuts at: 0 513 723
Size: 513 210
PstI C_TGCA'G
Cuts at: 0 311 473 545 723
Size: 311 162 72 178
Fragments arranged by size:
311 178 162 72
PvuII CAG'CTG
Cuts at: 0 537 723
Size: 537 186
SfcI C'TryA_G
Cuts at: 0 307 469 541 723
Size: 307 162 72 182
Fragments arranged by size:
307 182 162 72
SspI AAT'ATT
Cuts at: 0 393 723
Size: 393 330
StuI AGG'CCT
Cuts at: 0 547 723
Size: 547 176
StyI C'CwwG_G
Cuts at: 0 137 723
Size: 137 586
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 613 645 723
Size: 613 32 78
Fragments arranged by size:
613 78 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 585 714 723
Size: 585 129 9
Fragments arranged by size:
585 129 9
Enzymes that do cut:
AleI AlwNI ApoI BanI BbsI BglI BlpI BmrI
BpmI Bpu10I BsaAI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BsrFI BssSI BstAPI BstXI
Bsu36I BtgI BtsI DrdI EaeI Eco57MI EcoNI EcoO109I
HincII MmeI MscI MslI MspA1I NaeI NcoI NgoMIV
PflMI PpuMI PstI PvuII SfcI SspI StuI StyI
TstI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AlfI AloI ApaI ApaLI AscI
AseI AsiSI AvaI AvrII BaeI BamHI BanII BbeI
BbvCI BcgI BciVI BclI BfrBI BglII Bme1580I BmgBI
BmtI BplI BpuEI BsaI BsaBI BsaHI BsaXI BsiWI
BsmI BsmBI BspMI BsrBI BsrDI BsrGI BssHII BstBI
BstEII BstYI BstZ17I BtgZI ClaI CspCI DraI DraIII
EagI EarI EciI EcoICRI EcoRI EcoRV FalI FseI
FspI FspAI HaeII Hin4I HindIII HpaI KasI KpnI
MfeI MluI NarI NdeI NheI NotI NruI NsiI
NspI PacI PasI PciI PfoI PmeI PmlI PpiI
PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SwaI TaqII TatI Tth111I XbaI XhoI XmaI
XmnI ZraI