Restriction map for
pF1KB5227
(Linear) (Six-base) MAPSORT of: KIBB5227.seq Check: 8287 from: 1 to: 1482
KIBB5227.orf
With 182 enzymes: SgfI *
February 28, 2007 11:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 765 1482
Size: 765 717
AccI GT'mk_AC
Cuts at: 0 133 1030 1482
Size: 133 897 452
Fragments arranged by size:
897 452 133
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 46 1482
Size: 46 1436
AlwNI CAG_nnn'CTG
Cuts at: 0 142 772 1482
Size: 142 630 710
Fragments arranged by size:
710 630 142
ApoI r'AATT_y
Cuts at: 0 698 1482
Size: 698 784
AseI AT'TA_AT
Cuts at: 0 487 1482
Size: 487 995
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1174 1207 1482
Size: 1174 33 275
Fragments arranged by size:
1174 275 33
BanI G'GyrC_C
Cuts at: 0 1404 1482
Size: 1404 78
BbvCI CC'TCA_GC
Cuts at: 0 401 1482
Size: 401 1081
BciVI GTATCCnnnnn_n'
Cuts at: 0 1351 1482
Size: 1351 131
BlpI GC'TnA_GC
Cuts at: 0 147 1482
Size: 147 1335
Bme1580I G_kGCm'C
Cuts at: 0 1179 1409 1482
Size: 1179 230 73
Fragments arranged by size:
1179 230 73
BmgBI CAC'GTC
Cuts at: 0 691 1482
Size: 691 791
BmrI ACTGGGnnnn_n'
Cuts at: 0 570 809 1482
Size: 570 239 673
Fragments arranged by size:
673 570 239
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 455 776 1482
Size: 455 321 706
Fragments arranged by size:
706 455 321
Bpu10I CC'TnA_GC
Cuts at: 0 401 923 1482
Size: 401 522 559
Fragments arranged by size:
559 522 401
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 698 834 1482
Size: 698 136 648
Fragments arranged by size:
698 648 136
BsaI GGTCTCn'nnnn_
Cuts at: 0 1296 1443 1482
Size: 1296 147 39
Fragments arranged by size:
1296 147 39
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 494 1418 1482
Size: 494 924 64
Fragments arranged by size:
924 494 64
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 743 1482
Size: 743 739
BsiHKAI G_wGCw'C
Cuts at: 0 459 1482
Size: 459 1023
BsmI GAATG_Cn'
Cuts at: 0 470 524 665 1482
Size: 470 54 141 817
Fragments arranged by size:
817 470 141 54
Bsp1286I G_dGCh'C
Cuts at: 0 459 1179 1409 1482
Size: 459 720 230 73
Fragments arranged by size:
720 459 230 73
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 156 765 786 1482
Size: 156 609 21 696
Fragments arranged by size:
696 609 156 21
BsrDI GCAATG_nn'
Cuts at: 0 156 1482
Size: 156 1326
BstYI r'GATC_y
Cuts at: 0 71 1482
Size: 71 1411
BstZ17I GTA'TAC
Cuts at: 0 134 1482
Size: 134 1348
BtgI C'CryG_G
Cuts at: 0 31 1482
Size: 31 1451
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 533 568 1482
Size: 533 35 914
Fragments arranged by size:
914 533 35
DraI TTT'AAA
Cuts at: 0 105 1482
Size: 105 1377
EaeI y'GGCC_r
Cuts at: 0 1210 1473 1482
Size: 1210 263 9
Fragments arranged by size:
1210 263 9
EarI CTCTTCn'nnn_
Cuts at: 0 1160 1482
Size: 1160 322
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 46 455 776 1482
Size: 46 409 321 706
Fragments arranged by size:
706 409 321 46
EcoNI CCTnn'n_nnAGG
Cuts at: 0 404 632 1211 1482
Size: 404 228 579 271
Fragments arranged by size:
579 404 271 228
EcoO109I rG'GnC_Cy
Cuts at: 0 139 940 1221 1482
Size: 139 801 281 261
Fragments arranged by size:
801 281 261 139
EcoRI G'AATT_C
Cuts at: 0 698 1482
Size: 698 784
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 875 1482
Size: 875 607
HaeII r_GCGC'y
Cuts at: 0 736 1482
Size: 736 746
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 343 375 1141 1173 1482
Size: 343 32 766 32 309
Fragments arranged by size:
766 343 309 32 32
MfeI C'AATT_G
Cuts at: 0 1250 1482
Size: 1250 232
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 209 1224 1482
Size: 209 1015 258
Fragments arranged by size:
1015 258 209
MslI CAynn'nnrTG
Cuts at: 0 601 1145 1482
Size: 601 544 337
Fragments arranged by size:
601 544 337
MspA1I CmG'CkG
Cuts at: 0 24 931 1078 1482
Size: 24 907 147 404
Fragments arranged by size:
907 404 147 24
NspI r_CATG'y
Cuts at: 0 165 543 1353 1482
Size: 165 378 810 129
Fragments arranged by size:
810 378 165 129
PflMI CCAn_nnn'nTGG
Cuts at: 0 613 694 1243 1321 1482
Size: 613 81 549 78 161
Fragments arranged by size:
613 549 161 81 78
PfoI T'CCnGG_A
Cuts at: 0 693 1482
Size: 693 789
PpuMI rG'GwC_Cy
Cuts at: 0 139 940 1482
Size: 139 801 542
Fragments arranged by size:
801 542 139
PshAI GACnn'nnGTC
Cuts at: 0 838 1482
Size: 838 644
PstI C_TGCA'G
Cuts at: 0 366 1068 1482
Size: 366 702 414
Fragments arranged by size:
702 414 366
PvuII CAG'CTG
Cuts at: 0 24 931 1482
Size: 24 907 551
Fragments arranged by size:
907 551 24
ScaI AGT'ACT
Cuts at: 0 1062 1482
Size: 1062 420
SexAI A'CCwGG_T
Cuts at: 0 666 1482
Size: 666 816
SfcI C'TryA_G
Cuts at: 0 362 989 1031 1064 1482
Size: 362 627 42 33 418
Fragments arranged by size:
627 418 362 42 33
SmlI C'TyrA_G
Cuts at: 0 713 849 1482
Size: 713 136 633
Fragments arranged by size:
713 633 136
SpeI A'CTAG_T
Cuts at: 0 1045 1117 1482
Size: 1045 72 365
Fragments arranged by size:
1045 365 72
SphI G_CATG'C
Cuts at: 0 165 543 1353 1482
Size: 165 378 810 129
Fragments arranged by size:
810 378 165 129
StyI C'CwwG_G
Cuts at: 0 936 1482
Size: 936 546
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 589 601 1482
Size: 589 12 881
Fragments arranged by size:
881 589 12
TatI w'GTAC_w
Cuts at: 0 853 1048 1060 1482
Size: 853 195 12 422
Fragments arranged by size:
853 422 195 12
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 943 1482
Size: 943 539
XmnI GAAnn'nnTTC
Cuts at: 0 251 1482
Size: 251 1231
Enzymes that do cut:
AarI AccI AcuI AlwNI ApoI AseI BaeI BanI
BbvCI BciVI BlpI Bme1580I BmgBI BmrI BpmI Bpu10I
BpuEI BsaI BseRI BsgI BsiHKAI BsmI Bsp1286I BspMI
BsrDI BstYI BstZ17I BtgI CspCI DraI EaeI EarI
Eco57MI EcoNI EcoO109I EcoRI FalI HaeII Hin4I MfeI
MmeI MslI MspA1I NspI PflMI PfoI PpuMI PshAI
PstI PvuII ScaI SexAI SfcI SmlI SpeI SphI
StyI TaqII TatI XcmI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AleI AlfI AloI ApaI ApaLI AscI AsiSI AvaI
AvrII BamHI BanII BbeI BbsI BcgI BclI BfrBI
BglI BglII BmtI BplI BsaAI BsaBI BsaHI BsaWI
BsaXI BseYI BsiEI BsiWI BsmBI BspEI BspHI BsrBI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
Bsu36I BtgZI BtsI ClaI DraIII DrdI EagI EciI
EcoICRI EcoRV FseI FspI FspAI HincII HindIII HpaI
KasI KpnI MluI MscI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI PacI PasI PciI
PmeI PmlI PpiI PsiI PspOMI PspXI PsrI PvuI
RsrII SacI SacII SalI SanDI SapI SbfI SfiI
SfoI SgfI SgrAI SmaI SnaBI SrfI SspI StuI
SwaI TstI Tth111I XbaI XhoI XmaI ZraI