(Linear) (Six-base) MAPSORT of: KIBB5229.seq  Check: 2155  from: 1  to: 879



KIBB5229.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:04  ..

ApoI r'AATT_y

Cuts at:      0     245     809     879 
   Size:        245     564      70

  Fragments arranged by size: 

                 564     245      70
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     303     336     879 
   Size:        303      33     543

  Fragments arranged by size: 

                 543     303      33
 
BamHI G'GATC_C

Cuts at:      0     157     879 
   Size:        157     722
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     331     879 
   Size:        331     548
 
BclI T'GATC_A

Cuts at:      0     621     879 
   Size:        621     258
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     455     879 
   Size:        455     424
 
BsmI GAATG_Cn'

Cuts at:      0     398     879 
   Size:        398     481
 
BspHI T'CATG_A

Cuts at:      0     171     519     747     879 
   Size:        171     348     228     132

  Fragments arranged by size: 

                 348     228     171     132
 
BstEII G'GTnAC_C

Cuts at:      0     648     879 
   Size:        648     231
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     269     879 
   Size:        269     610
 
BstYI r'GATC_y

Cuts at:      0     157     879 
   Size:        157     722
 
DraI TTT'AAA

Cuts at:      0      99     713     879 
   Size:         99     614     166

  Fragments arranged by size: 

                 614     166      99
 
MslI CAynn'nnrTG

Cuts at:      0     795     879 
   Size:        795      84
 
MspA1I CmG'CkG

Cuts at:      0     327     879 
   Size:        327     552
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     810     842     879 
   Size:        810      32      37

  Fragments arranged by size: 

                 810      37      32
 
PvuII CAG'CTG

Cuts at:      0     327     879 
   Size:        327     552
 
SspI AAT'ATT

Cuts at:      0     372     879 
   Size:        372     507
 
StyI C'CwwG_G

Cuts at:      0      41     879 
   Size:         41     838
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     636     879 
   Size:        636     243
 
XmnI GAAnn'nnTTC

Cuts at:      0     289     879 
   Size:        289     590
 

 Enzymes that do cut:

     ApoI     BaeI    BamHI    BciVI     BclI     BsgI     BsmI    BspHI
   BstEII    BstXI    BstYI     DraI     MslI   MspA1I     PpiI    PvuII
     SspI     StyI    TaqII     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     AscI     AseI    AsiSI     AvaI    AvrII     BanI    BanII
     BbeI     BbsI    BbvCI     BcgI    BfrBI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
    BsiEI  BsiHKAI    BsiWI    BsmBI Bsp1286I    BspEI    BspMI    BsrBI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI  BstZ17I
   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI   DraIII     DrdI
     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII    Hin4I
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI
     MscI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PstI     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     StuI     SwaI
     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI