Restriction map for
pF1KB5232
(Linear) (Six-base) MAPSORT of: KIBB5232.seq Check: 5242 from: 1 to: 1506
KIBB5232.orf
With 182 enzymes: SgfI *
April 24, 2009 13:04 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1190 1506
Size: 1190 316
Acc65I G'GTAC_C
Cuts at: 0 1175 1192 1506
Size: 1175 17 314
Fragments arranged by size:
1175 314 17
AclI AA'CG_TT
Cuts at: 0 1136 1506
Size: 1136 370
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 521 655 726 1395 1462 1506
Size: 521 134 71 669 67 44
Fragments arranged by size:
669 521 134 71 67 44
AlwNI CAG_nnn'CTG
Cuts at: 0 630 633 749 1506
Size: 630 3 116 757
Fragments arranged by size:
757 630 116 3
ApaI G_GGCC'C
Cuts at: 0 140 1506
Size: 140 1366
ApoI r'AATT_y
Cuts at: 0 712 1402 1506
Size: 712 690 104
Fragments arranged by size:
712 690 104
AvaI C'yCGr_G
Cuts at: 0 1064 1506
Size: 1064 442
BanI G'GyrC_C
Cuts at: 0 113 143 1175 1192 1506
Size: 113 30 1032 17 314
Fragments arranged by size:
1032 314 113 30 17
BanII G_rGCy'C
Cuts at: 0 19 140 969 1506
Size: 19 121 829 537
Fragments arranged by size:
829 537 121 19
BbeI G_GCGC'C
Cuts at: 0 117 147 1506
Size: 117 30 1359
Fragments arranged by size:
1359 117 30
BglI GCCn_nnn'nGGC
Cuts at: 0 37 1506
Size: 37 1469
BlpI GC'TnA_GC
Cuts at: 0 1435 1506
Size: 1435 71
Bme1580I G_kGCm'C
Cuts at: 0 73 140 1506
Size: 73 67 1366
Fragments arranged by size:
1366 73 67
BmrI ACTGGGnnnn_n'
Cuts at: 0 76 1465 1506
Size: 76 1389 41
Fragments arranged by size:
1389 76 41
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 217 1300 1506
Size: 217 1083 206
Fragments arranged by size:
1083 217 206
BsaBI GATnn'nnATC
Cuts at: 0 806 1506
Size: 806 700
BsaHI Gr'CG_yC
Cuts at: 0 114 144 1506
Size: 114 30 1362
Fragments arranged by size:
1362 114 30
BsaWI w'CCGG_w
Cuts at: 0 1266 1506
Size: 1266 240
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 496 529 1506
Size: 496 33 977
Fragments arranged by size:
977 496 33
BseYI C'CCAG_C
Cuts at: 0 1171 1506
Size: 1171 335
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1126 1207 1506
Size: 1126 81 299
Fragments arranged by size:
1126 299 81
BsiHKAI G_wGCw'C
Cuts at: 0 1057 1506
Size: 1057 449
BsmI GAATG_Cn'
Cuts at: 0 473 1506
Size: 473 1033
Bsp1286I G_dGCh'C
Cuts at: 0 19 73 140 969 1057 1506
Size: 19 54 67 829 88 449
Fragments arranged by size:
829 449 88 67 54 19
BspEI T'CCGG_A
Cuts at: 0 1266 1506
Size: 1266 240
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1190 1506
Size: 1190 316
BstXI CCAn_nnnn'nTGG
Cuts at: 0 322 1506
Size: 322 1184
Bsu36I CC'TnA_GG
Cuts at: 0 1499 1506
Size: 1499 7
BtgI C'CryG_G
Cuts at: 0 700 1506
Size: 700 806
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 25 975 1506
Size: 25 950 531
Fragments arranged by size:
950 531 25
BtsI GCAGTG_nn'
Cuts at: 0 47 1506
Size: 47 1459
DraIII CAC_nnn'GTG
Cuts at: 0 1241 1506
Size: 1241 265
EaeI y'GGCC_r
Cuts at: 0 390 1035 1358 1506
Size: 390 645 323 148
Fragments arranged by size:
645 390 323 148
EarI CTCTTCn'nnn_
Cuts at: 0 451 745 928 1506
Size: 451 294 183 578
Fragments arranged by size:
578 451 294 183
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 217 521 655 726 1300 1395 1462 1506
Size: 217 304 134 71 574 95 67 44
Fragments arranged by size:
574 304 217 134 95 71 67 44
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1186 1506
Size: 1186 320
EcoO109I rG'GnC_Cy
Cuts at: 0 136 137 1230 1415 1506
Size: 136 1 1093 185 91
Fragments arranged by size:
1093 185 136 91 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 316 477 1506
Size: 316 161 1029
Fragments arranged by size:
1029 316 161
HaeII r_GCGC'y
Cuts at: 0 117 147 1222 1506
Size: 117 30 1075 284
Fragments arranged by size:
1075 284 117 30
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 3 35 341 373 1506
Size: 3 32 306 32 1133
Fragments arranged by size:
1133 306 32 32 3
KasI G'GCGC_C
Cuts at: 0 113 143 1506
Size: 113 30 1363
Fragments arranged by size:
1363 113 30
KpnI G_GTAC'C
Cuts at: 0 1179 1196 1506
Size: 1179 17 310
Fragments arranged by size:
1179 310 17
MscI TGG'CCA
Cuts at: 0 392 1037 1360 1506
Size: 392 645 323 146
Fragments arranged by size:
645 392 323 146
MspA1I CmG'CkG
Cuts at: 0 207 630 1506
Size: 207 423 876
Fragments arranged by size:
876 423 207
NarI GG'CG_CC
Cuts at: 0 114 144 1506
Size: 114 30 1362
Fragments arranged by size:
1362 114 30
NcoI C'CATG_G
Cuts at: 0 700 1506
Size: 700 806
NdeI CA'TA_TG
Cuts at: 0 779 1506
Size: 779 727
NspI r_CATG'y
Cuts at: 0 680 1343 1506
Size: 680 663 163
Fragments arranged by size:
680 663 163
PasI CC'CwG_GG
Cuts at: 0 1142 1506
Size: 1142 364
PflMI CCAn_nnn'nTGG
Cuts at: 0 1241 1278 1506
Size: 1241 37 228
Fragments arranged by size:
1241 228 37
PpuMI rG'GwC_Cy
Cuts at: 0 1230 1506
Size: 1230 276
PspOMI G'GGCC_C
Cuts at: 0 136 1506
Size: 136 1370
PstI C_TGCA'G
Cuts at: 0 264 816 910 1506
Size: 264 552 94 596
Fragments arranged by size:
596 552 264 94
PvuII CAG'CTG
Cuts at: 0 207 630 1506
Size: 207 423 876
Fragments arranged by size:
876 423 207
SanDI GG'GwC_CC
Cuts at: 0 1230 1506
Size: 1230 276
SapI GCTCTTCn'nnn_
Cuts at: 0 451 1506
Size: 451 1055
SfcI C'TryA_G
Cuts at: 0 260 812 906 1506
Size: 260 552 94 600
Fragments arranged by size:
600 552 260 94
SfoI GGC'GCC
Cuts at: 0 115 145 1506
Size: 115 30 1361
Fragments arranged by size:
1361 115 30
SmaI CCC'GGG
Cuts at: 0 1066 1506
Size: 1066 440
SphI G_CATG'C
Cuts at: 0 1343 1506
Size: 1343 163
StyI C'CwwG_G
Cuts at: 0 156 700 1187 1506
Size: 156 544 487 319
Fragments arranged by size:
544 487 319 156
TatI w'GTAC_w
Cuts at: 0 1008 1506
Size: 1008 498
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 855 887 1248 1280 1506
Size: 855 32 361 32 226
Fragments arranged by size:
855 361 226 32 32
XmaI C'CCGG_G
Cuts at: 0 1064 1506
Size: 1064 442
XmnI GAAnn'nnTTC
Cuts at: 0 473 1506
Size: 473 1033
Enzymes that do cut:
AarI Acc65I AclI AcuI AlwNI ApaI ApoI AvaI
BanI BanII BbeI BglI BlpI Bme1580I BmrI BpmI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiHKAI BsmI
Bsp1286I BspEI BspMI BstXI Bsu36I BtgI BtgZI BtsI
DraIII EaeI EarI Eco57MI EcoNI EcoO109I FalI HaeII
Hin4I KasI KpnI MscI MspA1I NarI NcoI NdeI
NspI PasI PflMI PpuMI PspOMI PstI PvuII SanDI
SapI SfcI SfoI SmaI SphI StyI TatI TstI
XmaI XmnI
Enzymes that do not cut:
AatII AccI AfeI AflII AflIII AgeI AhdI AleI
AlfI AloI ApaLI AscI AseI AsiSI AvrII BaeI
BamHI BbsI BbvCI BcgI BciVI BclI BfrBI BglII
BmgBI BmtI BplI Bpu10I BpuEI BsaI BsaAI BsaXI
BsiEI BsiWI BsmBI BspHI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I ClaI
CspCI DraI DrdI EagI EciI EcoICRI EcoRI EcoRV
FseI FspI FspAI HincII HindIII HpaI MfeI MluI
MmeI MslI NaeI NgoMIV NheI NotI NruI NsiI
PacI PciI PfoI PmeI PmlI PpiI PshAI PsiI
PspXI PsrI PvuI RsrII SacI SacII SalI SbfI
ScaI SexAI SfiI SgfI SgrAI SmlI SnaBI SpeI
SrfI SspI StuI SwaI TaqII Tth111I XbaI XcmI
XhoI ZraI