Restriction map for
pF1KB5234
(Linear) (Six-base) MAPSORT of: KIBB5234.seq Check: 8169 from: 1 to: 1602
KIBB5234.orf
With 182 enzymes: SgfI *
February 28, 2007 11:27 ..
AccI GT'mk_AC
Cuts at: 0 466 1602
Size: 466 1136
Acc65I G'GTAC_C
Cuts at: 0 860 1602
Size: 860 742
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 503 1602
Size: 503 1099
AfeI AGC'GCT
Cuts at: 0 538 1063 1602
Size: 538 525 539
Fragments arranged by size:
539 538 525
AflIII A'CryG_T
Cuts at: 0 1463 1602
Size: 1463 139
AhdI GACnn_n'nnGTC
Cuts at: 0 1515 1602
Size: 1515 87
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 706 1602
Size: 706 896
AlwNI CAG_nnn'CTG
Cuts at: 0 178 427 1134 1275 1602
Size: 178 249 707 141 327
Fragments arranged by size:
707 327 249 178 141
ApaI G_GGCC'C
Cuts at: 0 291 606 1602
Size: 291 315 996
Fragments arranged by size:
996 315 291
ApaLI G'TGCA_C
Cuts at: 0 982 1602
Size: 982 620
AvaI C'yCGr_G
Cuts at: 0 380 644 1406 1602
Size: 380 264 762 196
Fragments arranged by size:
762 380 264 196
BanI G'GyrC_C
Cuts at: 0 23 860 1602
Size: 23 837 742
Fragments arranged by size:
837 742 23
BanII G_rGCy'C
Cuts at: 0 16 291 413 606 1415 1602
Size: 16 275 122 193 809 187
Fragments arranged by size:
809 275 193 187 122 16
BbeI G_GCGC'C
Cuts at: 0 27 1602
Size: 27 1575
BbvCI CC'TCA_GC
Cuts at: 0 950 1602
Size: 950 652
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 261 295 1602
Size: 261 34 1307
Fragments arranged by size:
1307 261 34
BclI T'GATC_A
Cuts at: 0 1226 1602
Size: 1226 376
BglII A'GATC_T
Cuts at: 0 557 1602
Size: 557 1045
BlpI GC'TnA_GC
Cuts at: 0 179 1602
Size: 179 1423
Bme1580I G_kGCm'C
Cuts at: 0 291 606 986 1602
Size: 291 315 380 616
Fragments arranged by size:
616 380 315 291
BmtI G_CTAG'C
Cuts at: 0 1423 1602
Size: 1423 179
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1505 1602
Size: 1505 97
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 919 1602
Size: 919 683
Bpu10I CC'TnA_GC
Cuts at: 0 950 1602
Size: 950 652
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1042 1076 1602
Size: 1042 34 526
Fragments arranged by size:
1042 526 34
BsaAI yAC'GTr
Cuts at: 0 234 1602
Size: 234 1368
BsaBI GATnn'nnATC
Cuts at: 0 1393 1602
Size: 1393 209
BsaHI Gr'CG_yC
Cuts at: 0 24 1602
Size: 24 1578
BsaWI w'CCGG_w
Cuts at: 0 704 1602
Size: 704 898
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 77 130 496 1602
Size: 77 53 366 1106
Fragments arranged by size:
1106 366 77 53
BseYI C'CCAG_C
Cuts at: 0 188 1014 1415 1602
Size: 188 826 401 187
Fragments arranged by size:
826 401 188 187
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 370 1602
Size: 370 1232
BsiHKAI G_wGCw'C
Cuts at: 0 185 233 986 1602
Size: 185 48 753 616
Fragments arranged by size:
753 616 185 48
Bsp1286I G_dGCh'C
Cuts at: 0 16 185 233 291 413 606 986 1415
Size: 16 169 48 58 122 193 380 429
Cuts at: 1415 1602
Size: 187
Fragments arranged by size:
429 380 193 187 169 122 58 48
16
BspEI T'CCGG_A
Cuts at: 0 704 1602
Size: 704 898
BspHI T'CATG_A
Cuts at: 0 521 1602
Size: 521 1081
BsrFI r'CCGG_y
Cuts at: 0 87 1031 1602
Size: 87 944 571
Fragments arranged by size:
944 571 87
BssSI C'ACGA_G
Cuts at: 0 508 1357 1602
Size: 508 849 245
Fragments arranged by size:
849 508 245
BstXI CCAn_nnnn'nTGG
Cuts at: 0 897 1572 1602
Size: 897 675 30
Fragments arranged by size:
897 675 30
BstYI r'GATC_y
Cuts at: 0 557 1022 1602
Size: 557 465 580
Fragments arranged by size:
580 557 465
BtgI C'CryG_G
Cuts at: 0 27 413 1602
Size: 27 386 1189
Fragments arranged by size:
1189 386 27
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1248 1602
Size: 1248 354
DraIII CAC_nnn'GTG
Cuts at: 0 1203 1602
Size: 1203 399
EarI CTCTTCn'nnn_
Cuts at: 0 401 1602
Size: 401 1201
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 74 1033 1602
Size: 74 959 569
Fragments arranged by size:
959 569 74
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 503 919 1602
Size: 503 416 683
Fragments arranged by size:
683 503 416
EcoNI CCTnn'n_nnAGG
Cuts at: 0 940 1602
Size: 940 662
EcoO109I rG'GnC_Cy
Cuts at: 0 288 603 945 1131 1169 1602
Size: 288 315 342 186 38 433
Fragments arranged by size:
433 342 315 288 186 38
EcoRV GAT'ATC
Cuts at: 0 1455 1602
Size: 1455 147
FspI TGC'GCA
Cuts at: 0 682 1602
Size: 682 920
HaeII r_GCGC'y
Cuts at: 0 27 540 1065 1602
Size: 27 513 525 537
Fragments arranged by size:
537 525 513 27
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 573 605 1501 1533 1602
Size: 573 32 896 32 69
Fragments arranged by size:
896 573 69 32 32
HincII GTy'rAC
Cuts at: 0 140 467 1602
Size: 140 327 1135
Fragments arranged by size:
1135 327 140
HindIII A'AGCT_T
Cuts at: 0 988 1602
Size: 988 614
KasI G'GCGC_C
Cuts at: 0 23 1602
Size: 23 1579
KpnI G_GTAC'C
Cuts at: 0 864 1602
Size: 864 738
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 525 1262 1602
Size: 525 737 340
Fragments arranged by size:
737 525 340
MslI CAynn'nnrTG
Cuts at: 0 520 1000 1602
Size: 520 480 602
Fragments arranged by size:
602 520 480
MspA1I CmG'CkG
Cuts at: 0 29 175 897 961 1602
Size: 29 146 722 64 641
Fragments arranged by size:
722 641 146 64 29
NaeI GCC'GGC
Cuts at: 0 89 1602
Size: 89 1513
NarI GG'CG_CC
Cuts at: 0 24 1602
Size: 24 1578
NcoI C'CATG_G
Cuts at: 0 413 1602
Size: 413 1189
NgoMIV G'CCGG_C
Cuts at: 0 87 1602
Size: 87 1515
NheI G'CTAG_C
Cuts at: 0 1419 1602
Size: 1419 183
NspI r_CATG'y
Cuts at: 0 697 1467 1602
Size: 697 770 135
Fragments arranged by size:
770 697 135
PciI A'CATG_T
Cuts at: 0 1463 1602
Size: 1463 139
PflMI CCAn_nnn'nTGG
Cuts at: 0 102 1134 1602
Size: 102 1032 468
Fragments arranged by size:
1032 468 102
PfoI T'CCnGG_A
Cuts at: 0 401 659 1133 1325 1602
Size: 401 258 474 192 277
Fragments arranged by size:
474 401 277 258 192
PmlI CAC'GTG
Cuts at: 0 234 1602
Size: 234 1368
PpuMI rG'GwC_Cy
Cuts at: 0 1131 1169 1602
Size: 1131 38 433
Fragments arranged by size:
1131 433 38
PspOMI G'GGCC_C
Cuts at: 0 287 602 1602
Size: 287 315 1000
Fragments arranged by size:
1000 315 287
PstI C_TGCA'G
Cuts at: 0 1040 1280 1524 1602
Size: 1040 240 244 78
Fragments arranged by size:
1040 244 240 78
PvuII CAG'CTG
Cuts at: 0 897 961 1602
Size: 897 64 641
Fragments arranged by size:
897 641 64
SacII CC_GC'GG
Cuts at: 0 30 1602
Size: 30 1572
SalI G'TCGA_C
Cuts at: 0 465 1602
Size: 465 1137
SapI GCTCTTCn'nnn_
Cuts at: 0 401 1602
Size: 401 1201
ScaI AGT'ACT
Cuts at: 0 588 904 1602
Size: 588 316 698
Fragments arranged by size:
698 588 316
SexAI A'CCwGG_T
Cuts at: 0 1127 1602
Size: 1127 475
SfcI C'TryA_G
Cuts at: 0 780 1036 1276 1520 1602
Size: 780 256 240 244 82
Fragments arranged by size:
780 256 244 240 82
SfoI GGC'GCC
Cuts at: 0 25 1602
Size: 25 1577
SmaI CCC'GGG
Cuts at: 0 646 1602
Size: 646 956
SmlI C'TyrA_G
Cuts at: 0 380 1057 1091 1602
Size: 380 677 34 511
Fragments arranged by size:
677 511 380 34
SphI G_CATG'C
Cuts at: 0 697 1602
Size: 697 905
StyI C'CwwG_G
Cuts at: 0 212 413 1253 1316 1379 1565 1602
Size: 212 201 840 63 63 186 37
Fragments arranged by size:
840 212 201 186 63 63 37
TatI w'GTAC_w
Cuts at: 0 586 902 1602
Size: 586 316 700
Fragments arranged by size:
700 586 316
Tth111I GACn'n_nGTC
Cuts at: 0 205 1602
Size: 205 1397
XhoI C'TCGA_G
Cuts at: 0 380 1602
Size: 380 1222
XmaI C'CCGG_G
Cuts at: 0 644 1602
Size: 644 958
Enzymes that do cut:
AccI Acc65I AcuI AfeI AflIII AhdI AlfI AlwNI
ApaI ApaLI AvaI BanI BanII BbeI BbvCI BcgI
BclI BglII BlpI Bme1580I BmtI BplI BpmI Bpu10I
BpuEI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI
BsiHKAI Bsp1286I BspEI BspHI BsrFI BssSI BstXI BstYI
BtgI BtgZI DraIII EarI EciI Eco57MI EcoNI EcoO109I
EcoRV FspI HaeII Hin4I HincII HindIII KasI KpnI
MmeI MslI MspA1I NaeI NarI NcoI NgoMIV NheI
NspI PciI PflMI PfoI PmlI PpuMI PspOMI PstI
PvuII SacII SalI SapI ScaI SexAI SfcI SfoI
SmaI SmlI SphI StyI TatI Tth111I XhoI XmaI
Enzymes that do not cut:
AarI AatII AclI AflII AgeI AleI AloI ApoI
AscI AseI AsiSI AvrII BaeI BamHI BbsI BciVI
BfrBI BglI BmgBI BmrI BsaI BsaXI BsiEI BsiWI
BsmI BsmBI BspMI BsrBI BsrDI BsrGI BssHII BstAPI
BstBI BstEII BstZ17I Bsu36I BtsI ClaI CspCI DraI
DrdI EaeI EagI EcoICRI EcoRI FalI FseI FspAI
HpaI MfeI MluI MscI NdeI NotI NruI NsiI
PacI PasI PmeI PpiI PshAI PsiI PspXI PsrI
PvuI RsrII SacI SanDI SbfI SfiI SgfI SgrAI
SnaBI SpeI SrfI SspI StuI SwaI TaqII TstI
XbaI XcmI XmnI ZraI