Restriction map for
pF1KB5236
(Linear) (Six-base) MAPSORT of: KIBB5236.seq Check: 8086 from: 1 to: 1062
KIBB5236.orf
With 182 enzymes: SgfI *
April 24, 2009 13:04 ..
AccI GT'mk_AC
Cuts at: 0 9 1040 1062
Size: 9 1031 22
Fragments arranged by size:
1031 22 9
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 361 1032 1062
Size: 361 671 30
Fragments arranged by size:
671 361 30
AleI CACnn'nnGTG
Cuts at: 0 158 1062
Size: 158 904
ApoI r'AATT_y
Cuts at: 0 184 890 1062
Size: 184 706 172
Fragments arranged by size:
706 184 172
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 434 467 1062
Size: 434 33 595
Fragments arranged by size:
595 434 33
BanII G_rGCy'C
Cuts at: 0 674 1062
Size: 674 388
BciVI GTATCCnnnnn_n'
Cuts at: 0 289 1062
Size: 289 773
BclI T'GATC_A
Cuts at: 0 45 1062
Size: 45 1017
BglI GCCn_nnn'nGGC
Cuts at: 0 966 1062
Size: 966 96
Bme1580I G_kGCm'C
Cuts at: 0 698 1062
Size: 698 364
BmrI ACTGGGnnnn_n'
Cuts at: 0 508 1062
Size: 508 554
Bpu10I CC'TnA_GC
Cuts at: 0 213 1062
Size: 213 849
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 250 1062
Size: 250 812
BsaI GGTCTCn'nnnn_
Cuts at: 0 188 1062
Size: 188 874
BsaBI GATnn'nnATC
Cuts at: 0 31 1062
Size: 31 1031
BsaHI Gr'CG_yC
Cuts at: 0 587 1062
Size: 587 475
BseYI C'CCAG_C
Cuts at: 0 38 1062
Size: 38 1024
BsiHKAI G_wGCw'C
Cuts at: 0 852 1062
Size: 852 210
Bsp1286I G_dGCh'C
Cuts at: 0 674 698 852 1062
Size: 674 24 154 210
Fragments arranged by size:
674 210 154 24
BspHI T'CATG_A
Cuts at: 0 75 1062
Size: 75 987
BsrDI GCAATG_nn'
Cuts at: 0 171 621 1062
Size: 171 450 441
Fragments arranged by size:
450 441 171
BsrFI r'CCGG_y
Cuts at: 0 619 1062
Size: 619 443
BssSI C'ACGA_G
Cuts at: 0 523 1062
Size: 523 539
BtgI C'CryG_G
Cuts at: 0 290 1062
Size: 290 772
BtsI GCAGTG_nn'
Cuts at: 0 172 357 998 1062
Size: 172 185 641 64
Fragments arranged by size:
641 185 172 64
EaeI y'GGCC_r
Cuts at: 0 774 1062
Size: 774 288
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 943 1062
Size: 943 119
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 361 1032 1062
Size: 361 671 30
Fragments arranged by size:
671 361 30
HaeII r_GCGC'y
Cuts at: 0 507 972 1062
Size: 507 465 90
Fragments arranged by size:
507 465 90
HindIII A'AGCT_T
Cuts at: 0 830 1062
Size: 830 232
MslI CAynn'nnrTG
Cuts at: 0 158 177 1062
Size: 158 19 885
Fragments arranged by size:
885 158 19
MspA1I CmG'CkG
Cuts at: 0 579 908 1062
Size: 579 329 154
Fragments arranged by size:
579 329 154
NaeI GCC'GGC
Cuts at: 0 621 1062
Size: 621 441
NcoI C'CATG_G
Cuts at: 0 290 1062
Size: 290 772
NgoMIV G'CCGG_C
Cuts at: 0 619 1062
Size: 619 443
PstI C_TGCA'G
Cuts at: 0 976 993 1062
Size: 976 17 69
Fragments arranged by size:
976 69 17
PvuII CAG'CTG
Cuts at: 0 579 1062
Size: 579 483
SfcI C'TryA_G
Cuts at: 0 200 972 989 1062
Size: 200 772 17 73
Fragments arranged by size:
772 200 73 17
SmlI C'TyrA_G
Cuts at: 0 265 1062
Size: 265 797
StyI C'CwwG_G
Cuts at: 0 140 290 1062
Size: 140 150 772
Fragments arranged by size:
772 150 140
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 103 1062
Size: 103 959
Enzymes that do cut:
AccI AcuI AleI ApoI BaeI BanII BciVI BclI
BglI Bme1580I BmrI Bpu10I BpuEI BsaI BsaBI BsaHI
BseYI BsiHKAI Bsp1286I BspHI BsrDI BsrFI BssSI BtgI
BtsI EaeI EciI Eco57MI HaeII HindIII MslI MspA1I
NaeI NcoI NgoMIV PstI PvuII SfcI SmlI StyI
XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AlfI AloI AlwNI ApaI ApaLI AscI AseI
AsiSI AvaI AvrII BamHI BanI BbeI BbsI BbvCI
BcgI BfrBI BglII BlpI BmgBI BmtI BplI BpmI
BsaAI BsaWI BsaXI BseRI BsgI BsiEI BsiWI BsmI
BsmBI BspEI BspMI BsrBI BsrGI BssHII BstAPI BstBI
BstEII BstXI BstYI BstZ17I Bsu36I BtgZI ClaI CspCI
DraI DraIII DrdI EagI EarI EcoICRI EcoNI EcoO109I
EcoRI EcoRV FalI FseI FspI FspAI Hin4I HincII
HpaI KasI KpnI MfeI MluI MmeI MscI NarI
NdeI NheI NotI NruI NsiI NspI PacI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
StuI SwaI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI