Restriction map for
pF1KB5237
(Linear) (Six-base) MAPSORT of: KIBB5237.seq Check: 5009 from: 1 to: 885
KIBB5237.orf
With 182 enzymes: SgfI *
April 24, 2009 13:04 ..
AccI GT'mk_AC
Cuts at: 0 194 885
Size: 194 691
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 80 885
Size: 80 805
AgeI A'CCGG_T
Cuts at: 0 352 885
Size: 352 533
ApoI r'AATT_y
Cuts at: 0 742 885
Size: 742 143
AvaI C'yCGr_G
Cuts at: 0 20 34 885
Size: 20 14 851
Fragments arranged by size:
851 20 14
BbsI GAAGACnn'nnnn_
Cuts at: 0 66 312 885
Size: 66 246 573
Fragments arranged by size:
573 246 66
BglI GCCn_nnn'nGGC
Cuts at: 0 85 885
Size: 85 800
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 427 885
Size: 427 458
Bpu10I CC'TnA_GC
Cuts at: 0 517 885
Size: 517 368
BsaI GGTCTCn'nnnn_
Cuts at: 0 31 885
Size: 31 854
BsaWI w'CCGG_w
Cuts at: 0 352 692 885
Size: 352 340 193
Fragments arranged by size:
352 340 193
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 358 388 761 791 885
Size: 358 30 373 30 94
Fragments arranged by size:
373 358 94 30 30
BseYI C'CCAG_C
Cuts at: 0 68 361 885
Size: 68 293 524
Fragments arranged by size:
524 293 68
BsiHKAI G_wGCw'C
Cuts at: 0 352 885
Size: 352 533
Bsp1286I G_dGCh'C
Cuts at: 0 352 885
Size: 352 533
BsrFI r'CCGG_y
Cuts at: 0 88 352 885
Size: 88 264 533
Fragments arranged by size:
533 264 88
BtsI GCAGTG_nn'
Cuts at: 0 674 885
Size: 674 211
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 95 130 885
Size: 95 35 755
Fragments arranged by size:
755 95 35
DraIII CAC_nnn'GTG
Cuts at: 0 263 885
Size: 263 622
EaeI y'GGCC_r
Cuts at: 0 86 885
Size: 86 799
EarI CTCTTCn'nnn_
Cuts at: 0 691 885
Size: 691 194
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 80 427 885
Size: 80 347 458
Fragments arranged by size:
458 347 80
EcoO109I rG'GnC_Cy
Cuts at: 0 24 885
Size: 24 861
EcoRI G'AATT_C
Cuts at: 0 742 885
Size: 742 143
HaeII r_GCGC'y
Cuts at: 0 95 885
Size: 95 790
HincII GTy'rAC
Cuts at: 0 690 885
Size: 690 195
HpaI GTT'AAC
Cuts at: 0 690 885
Size: 690 195
NaeI GCC'GGC
Cuts at: 0 90 885
Size: 90 795
NgoMIV G'CCGG_C
Cuts at: 0 88 885
Size: 88 797
PfoI T'CCnGG_A
Cuts at: 0 524 885
Size: 524 361
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 339 371 885
Size: 339 32 514
Fragments arranged by size:
514 339 32
PpuMI rG'GwC_Cy
Cuts at: 0 24 885
Size: 24 861
PshAI GACnn'nnGTC
Cuts at: 0 43 493 885
Size: 43 450 392
Fragments arranged by size:
450 392 43
SanDI GG'GwC_CC
Cuts at: 0 24 885
Size: 24 861
SmaI CCC'GGG
Cuts at: 0 22 885
Size: 22 863
SspI AAT'ATT
Cuts at: 0 755 885
Size: 755 130
TatI w'GTAC_w
Cuts at: 0 700 885
Size: 700 185
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 446 478 885
Size: 446 32 407
Fragments arranged by size:
446 407 32
XmaI C'CCGG_G
Cuts at: 0 20 885
Size: 20 865
Enzymes that do cut:
AccI AcuI AgeI ApoI AvaI BbsI BglI BpmI
Bpu10I BsaI BsaWI BsaXI BseYI BsiHKAI Bsp1286I BsrFI
BtsI CspCI DraIII EaeI EarI Eco57MI EcoO109I EcoRI
HaeII HincII HpaI NaeI NgoMIV PfoI PpiI PpuMI
PshAI SanDI SmaI SspI TatI TstI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AhdI
AleI AlfI AloI AlwNI ApaI ApaLI AscI AseI
AsiSI AvrII BaeI BamHI BanI BanII BbeI BbvCI
BcgI BciVI BclI BfrBI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpuEI BsaAI BsaBI BsaHI BseRI
BsgI BsiEI BsiWI BsmI BsmBI BspEI BspHI BspMI
BsrBI BsrDI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BstZ17I Bsu36I BtgI BtgZI ClaI DraI
DrdI EagI EciI EcoICRI EcoNI EcoRV FalI FseI
FspI FspAI Hin4I HindIII KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NruI NsiI NspI PacI PasI PciI PflMI
PmeI PmlI PsiI PspOMI PspXI PsrI PstI PvuI
PvuII RsrII SacI SacII SalI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SgfI SgrAI SmlI SnaBI
SpeI SphI SrfI StuI StyI SwaI TaqII Tth111I
XbaI XcmI XhoI XmnI ZraI