Restriction map for
pF1KB5238
(Linear) (Six-base) MAPSORT of: KIBB5238.seq Check: 8294 from: 1 to: 621
KIBB5238.orf
With 182 enzymes: SgfI *
April 24, 2009 13:04 ..
AatII G_ACGT'C
Cuts at: 0 539 621
Size: 539 82
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 216 217 415 621
Size: 216 1 198 206
Fragments arranged by size:
216 206 198 1
ApaLI G'TGCA_C
Cuts at: 0 243 621
Size: 243 378
BanII G_rGCy'C
Cuts at: 0 100 240 621
Size: 100 140 381
Fragments arranged by size:
381 140 100
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 519 553 621
Size: 519 34 68
Fragments arranged by size:
519 68 34
BglI GCCn_nnn'nGGC
Cuts at: 0 317 621
Size: 317 304
Bme1580I G_kGCm'C
Cuts at: 0 247 621
Size: 247 374
BmgBI CAC'GTC
Cuts at: 0 153 519 621
Size: 153 366 102
Fragments arranged by size:
366 153 102
BmrI ACTGGGnnnn_n'
Cuts at: 0 450 621
Size: 450 171
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 211 621
Size: 211 410
BsaAI yAC'GTr
Cuts at: 0 402 621
Size: 402 219
BsaBI GATnn'nnATC
Cuts at: 0 595 621
Size: 595 26
BsaHI Gr'CG_yC
Cuts at: 0 158 536 621
Size: 158 378 85
Fragments arranged by size:
378 158 85
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 259 289 621
Size: 259 30 332
Fragments arranged by size:
332 259 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 111 114 621
Size: 111 3 507
Fragments arranged by size:
507 111 3
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 453 621
Size: 453 168
BsiEI CG_ry'CG
Cuts at: 0 180 621
Size: 180 441
BsiHKAI G_wGCw'C
Cuts at: 0 247 621
Size: 247 374
Bsp1286I G_dGCh'C
Cuts at: 0 100 240 247 621
Size: 100 140 7 374
Fragments arranged by size:
374 140 100 7
BsrBI CCG'CTC
Cuts at: 0 138 174 621
Size: 138 36 447
Fragments arranged by size:
447 138 36
BssSI C'ACGA_G
Cuts at: 0 139 621
Size: 139 482
BtgI C'CryG_G
Cuts at: 0 74 422 621
Size: 74 348 199
Fragments arranged by size:
348 199 74
DraIII CAC_nnn'GTG
Cuts at: 0 291 621
Size: 291 330
EaeI y'GGCC_r
Cuts at: 0 177 609 621
Size: 177 432 12
Fragments arranged by size:
432 177 12
EagI C'GGCC_G
Cuts at: 0 177 621
Size: 177 444
EarI CTCTTCn'nnn_
Cuts at: 0 120 621
Size: 120 501
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 211 216 217 415 621
Size: 211 5 1 198 206
Fragments arranged by size:
211 206 198 5 1
EcoNI CCTnn'n_nnAGG
Cuts at: 0 285 621
Size: 285 336
HaeII r_GCGC'y
Cuts at: 0 10 621
Size: 10 611
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 97 129 621
Size: 97 32 492
Fragments arranged by size:
492 97 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 470 621
Size: 470 151
MscI TGG'CCA
Cuts at: 0 611 621
Size: 611 10
MspA1I CmG'CkG
Cuts at: 0 26 63 76 223 621
Size: 26 37 13 147 398
Fragments arranged by size:
398 147 37 26 13
NcoI C'CATG_G
Cuts at: 0 422 621
Size: 422 199
NspI r_CATG'y
Cuts at: 0 505 621
Size: 505 116
PfoI T'CCnGG_A
Cuts at: 0 127 621
Size: 127 494
PmlI CAC'GTG
Cuts at: 0 402 621
Size: 402 219
PstI C_TGCA'G
Cuts at: 0 91 621
Size: 91 530
PvuII CAG'CTG
Cuts at: 0 223 621
Size: 223 398
SacII CC_GC'GG
Cuts at: 0 77 621
Size: 77 544
SfcI C'TryA_G
Cuts at: 0 87 621
Size: 87 534
StyI C'CwwG_G
Cuts at: 0 422 621
Size: 422 199
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 502 621
Size: 502 119
XmnI GAAnn'nnTTC
Cuts at: 0 259 621
Size: 259 362
ZraI GAC'GTC
Cuts at: 0 537 621
Size: 537 84
Enzymes that do cut:
AatII AcuI ApaLI BanII BcgI BglI Bme1580I BmgBI
BmrI BpmI BsaAI BsaBI BsaHI BsaXI BseRI BsgI
BsiEI BsiHKAI Bsp1286I BsrBI BssSI BtgI DraIII EaeI
EagI EarI Eco57MI EcoNI HaeII Hin4I MmeI MscI
MspA1I NcoI NspI PfoI PmlI PstI PvuII SacII
SfcI StyI TaqII XmnI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApoI AscI
AseI AsiSI AvaI AvrII BaeI BamHI BanI BbeI
BbsI BbvCI BciVI BclI BfrBI BglII BlpI BmtI
BplI Bpu10I BpuEI BsaI BsaWI BseYI BsiWI BsmI
BsmBI BspEI BspHI BspMI BsrDI BsrFI BsrGI BssHII
BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgZI
BtsI ClaI CspCI DraI DrdI EciI EcoICRI EcoO109I
EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII
HpaI KasI KpnI MfeI MluI MslI NaeI NarI
NdeI NgoMIV NheI NotI NruI NsiI PacI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI
PspXI PsrI PvuI RsrII SacI SalI SanDI SapI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI
TatI TstI Tth111I XbaI XcmI XhoI XmaI