Restriction map for
pF1KB5241
(Linear) (Six-base) MAPSORT of: KIBB5241.seq Check: 9752 from: 1 to: 1434
KIBB5241.orf
With 182 enzymes: SgfI *
April 24, 2009 13:04 ..
AatII G_ACGT'C
Cuts at: 0 1187 1434
Size: 1187 247
AccI GT'mk_AC
Cuts at: 0 1337 1434
Size: 1337 97
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 235 469 664 694 722 1097 1434
Size: 235 234 195 30 28 375 337
Fragments arranged by size:
375 337 235 234 195 30 28
AfeI AGC'GCT
Cuts at: 0 534 1434
Size: 534 900
AflIII A'CryG_T
Cuts at: 0 737 1434
Size: 737 697
ApaI G_GGCC'C
Cuts at: 0 1389 1434
Size: 1389 45
ApoI r'AATT_y
Cuts at: 0 130 1083 1165 1434
Size: 130 953 82 269
Fragments arranged by size:
953 269 130 82
BanII G_rGCy'C
Cuts at: 0 1389 1434
Size: 1389 45
BclI T'GATC_A
Cuts at: 0 18 1434
Size: 18 1416
BfrBI ATG'CAT
Cuts at: 0 1103 1434
Size: 1103 331
BglI GCCn_nnn'nGGC
Cuts at: 0 566 933 1434
Size: 566 367 501
Fragments arranged by size:
566 501 367
BglII A'GATC_T
Cuts at: 0 1305 1434
Size: 1305 129
Bme1580I G_kGCm'C
Cuts at: 0 1389 1434
Size: 1389 45
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 61 1390 1434
Size: 61 1329 44
Fragments arranged by size:
1329 61 44
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 832 1338 1434
Size: 832 506 96
Fragments arranged by size:
832 506 96
BsaBI GATnn'nnATC
Cuts at: 0 20 901 1434
Size: 20 881 533
Fragments arranged by size:
881 533 20
BsaHI Gr'CG_yC
Cuts at: 0 1184 1434
Size: 1184 250
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 291 1434
Size: 291 1143
BseYI C'CCAG_C
Cuts at: 0 535 1434
Size: 535 899
Bsp1286I G_dGCh'C
Cuts at: 0 1389 1434
Size: 1389 45
BsrBI CCG'CTC
Cuts at: 0 1383 1434
Size: 1383 51
BstEII G'GTnAC_C
Cuts at: 0 285 1434
Size: 285 1149
BstYI r'GATC_y
Cuts at: 0 1305 1434
Size: 1305 129
Bsu36I CC'TnA_GG
Cuts at: 0 616 1434
Size: 616 818
BtsI GCAGTG_nn'
Cuts at: 0 1001 1434
Size: 1001 433
DraI TTT'AAA
Cuts at: 0 573 1208 1434
Size: 573 635 226
Fragments arranged by size:
635 573 226
EarI CTCTTCn'nnn_
Cuts at: 0 211 1073 1434
Size: 211 862 361
Fragments arranged by size:
862 361 211
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 61 235 469 664 694 722 1097 1390
Size: 61 174 234 195 30 28 375 293
Cuts at: 1390 1434
Size: 44
Fragments arranged by size:
375 293 234 195 174 61 44 30
28
EcoNI CCTnn'n_nnAGG
Cuts at: 0 355 619 1434
Size: 355 264 815
Fragments arranged by size:
815 355 264
EcoO109I rG'GnC_Cy
Cuts at: 0 1386 1429 1434
Size: 1386 43 5
Fragments arranged by size:
1386 43 5
EcoRI G'AATT_C
Cuts at: 0 130 1434
Size: 130 1304
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 504 1434
Size: 504 930
HaeII r_GCGC'y
Cuts at: 0 536 1434
Size: 536 898
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1413 1434
Size: 1413 21
HindIII A'AGCT_T
Cuts at: 0 379 676 1314 1434
Size: 379 297 638 120
Fragments arranged by size:
638 379 297 120
MfeI C'AATT_G
Cuts at: 0 46 1434
Size: 46 1388
NsiI A_TGCA'T
Cuts at: 0 1105 1434
Size: 1105 329
NspI r_CATG'y
Cuts at: 0 741 1434
Size: 741 693
PciI A'CATG_T
Cuts at: 0 737 1434
Size: 737 697
PflMI CCAn_nnn'nTGG
Cuts at: 0 646 1434
Size: 646 788
PpuMI rG'GwC_Cy
Cuts at: 0 1429 1434
Size: 1429 5
PshAI GACnn'nnGTC
Cuts at: 0 754 1434
Size: 754 680
PspOMI G'GGCC_C
Cuts at: 0 1385 1434
Size: 1385 49
PstI C_TGCA'G
Cuts at: 0 996 1434
Size: 996 438
SapI GCTCTTCn'nnn_
Cuts at: 0 211 1434
Size: 211 1223
ScaI AGT'ACT
Cuts at: 0 1375 1434
Size: 1375 59
SfcI C'TryA_G
Cuts at: 0 399 992 1434
Size: 399 593 442
Fragments arranged by size:
593 442 399
SmlI C'TyrA_G
Cuts at: 0 811 1317 1434
Size: 811 506 117
Fragments arranged by size:
811 506 117
StuI AGG'CCT
Cuts at: 0 484 829 1434
Size: 484 345 605
Fragments arranged by size:
605 484 345
StyI C'CwwG_G
Cuts at: 0 479 1326 1434
Size: 479 847 108
Fragments arranged by size:
847 479 108
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 858 1434
Size: 858 576
TatI w'GTAC_w
Cuts at: 0 4 713 1373 1434
Size: 4 709 660 61
Fragments arranged by size:
709 660 61 4
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 881 913 1434
Size: 881 32 521
Fragments arranged by size:
881 521 32
Tth111I GACn'n_nGTC
Cuts at: 0 1427 1434
Size: 1427 7
XbaI T'CTAG_A
Cuts at: 0 1029 1434
Size: 1029 405
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 150 1017 1434
Size: 150 867 417
Fragments arranged by size:
867 417 150
ZraI GAC'GTC
Cuts at: 0 1185 1434
Size: 1185 249
Enzymes that do cut:
AatII AccI AcuI AfeI AflIII ApaI ApoI BanII
BclI BfrBI BglI BglII Bme1580I BpmI BpuEI BsaBI
BsaHI BseRI BseYI Bsp1286I BsrBI BstEII BstYI Bsu36I
BtsI DraI EarI Eco57MI EcoNI EcoO109I EcoRI FalI
HaeII Hin4I HindIII MfeI NsiI NspI PciI PflMI
PpuMI PshAI PspOMI PstI SapI ScaI SfcI SmlI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
ZraI
Enzymes that do not cut:
AarI Acc65I AclI AflII AgeI AhdI AleI AlfI
AloI AlwNI ApaLI AscI AseI AsiSI AvaI AvrII
BaeI BamHI BanI BbeI BbsI BbvCI BcgI BciVI
BlpI BmgBI BmrI BmtI BplI Bpu10I BsaI BsaAI
BsaWI BsaXI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI
BspEI BspHI BspMI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstXI BstZ17I BtgI BtgZI ClaI CspCI
DraIII DrdI EaeI EagI EciI EcoICRI EcoRV FseI
FspI FspAI HincII HpaI KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI PacI PasI PfoI PmeI PmlI
PpiI PsiI PspXI PsrI PvuI PvuII RsrII SacI
SacII SalI SanDI SbfI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI
XhoI XmaI XmnI