Restriction map for
pF1KB5242
(Linear) (Six-base) MAPSORT of: KIBB5242.seq Check: 4540 from: 1 to: 1566
KIBB5242.orf
With 182 enzymes: SgfI *
February 28, 2007 11:27 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 891 1566
Size: 891 675
AflIII A'CryG_T
Cuts at: 0 476 1566
Size: 476 1090
AgeI A'CCGG_T
Cuts at: 0 668 1566
Size: 668 898
AleI CACnn'nnGTG
Cuts at: 0 1009 1566
Size: 1009 557
AlwNI CAG_nnn'CTG
Cuts at: 0 241 657 690 1155 1182 1455 1536 1566
Size: 241 416 33 465 27 273 81 30
Fragments arranged by size:
465 416 273 241 81 33 30 27
ApaI G_GGCC'C
Cuts at: 0 36 71 194 1416 1566
Size: 36 35 123 1222 150
Fragments arranged by size:
1222 150 123 36 35
AvaI C'yCGr_G
Cuts at: 0 29 36 70 833 1328 1407 1566
Size: 29 7 34 763 495 79 159
Fragments arranged by size:
763 495 159 79 34 29 7
AvrII C'CTAG_G
Cuts at: 0 1431 1566
Size: 1431 135
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 739 772 1023 1056 1566
Size: 739 33 251 33 510
Fragments arranged by size:
739 510 251 33 33
BamHI G'GATC_C
Cuts at: 0 824 1566
Size: 824 742
BanI G'GyrC_C
Cuts at: 0 665 849 896 958 1427 1566
Size: 665 184 47 62 469 139
Fragments arranged by size:
665 469 184 139 62 47
BanII G_rGCy'C
Cuts at: 0 36 71 194 283 683 915 1025 1408
Size: 36 35 123 89 400 232 110 383
Cuts at: 1408 1416 1566
Size: 8 150
Fragments arranged by size:
400 383 232 150 123 110 89 36
35 8
BbvCI CC'TCA_GC
Cuts at: 0 1136 1566
Size: 1136 430
BciVI GTATCCnnnnn_n'
Cuts at: 0 1024 1566
Size: 1024 542
BglI GCCn_nnn'nGGC
Cuts at: 0 771 1301 1388 1442 1566
Size: 771 530 87 54 124
Fragments arranged by size:
771 530 124 87 54
Bme1580I G_kGCm'C
Cuts at: 0 36 71 194 537 1070 1104 1416 1566
Size: 36 35 123 343 533 34 312 150
Fragments arranged by size:
533 343 312 150 123 36 35 34
BmrI ACTGGGnnnn_n'
Cuts at: 0 224 1566
Size: 224 1342
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 133 1566
Size: 133 1433
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 152 1039 1178 1498 1513 1566
Size: 152 887 139 320 15 53
Fragments arranged by size:
887 320 152 139 53 15
Bpu10I CC'TnA_GC
Cuts at: 0 1136 1566
Size: 1136 430
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 444 838 1489 1566
Size: 444 394 651 77
Fragments arranged by size:
651 444 394 77
BsaI GGTCTCn'nnnn_
Cuts at: 0 33 89 383 1325 1566
Size: 33 56 294 942 241
Fragments arranged by size:
942 294 241 56 33
BsaAI yAC'GTr
Cuts at: 0 477 633 1566
Size: 477 156 933
Fragments arranged by size:
933 477 156
BsaWI w'CCGG_w
Cuts at: 0 668 1566
Size: 668 898
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 67 97 436 466 1176 1206 1566
Size: 67 30 339 30 710 30 360
Fragments arranged by size:
710 360 339 67 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 18 236 1177 1566
Size: 18 218 941 389
Fragments arranged by size:
941 389 218 18
BseYI C'CCAG_C
Cuts at: 0 193 303 316 650 683 1079 1155 1401
Size: 193 110 13 334 33 396 76 246
Cuts at: 1401 1499 1547 1566
Size: 98 48 19
Fragments arranged by size:
396 334 246 193 110 98 76 48
33 19 13
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 254 469 901 1188 1566
Size: 254 215 432 287 378
Fragments arranged by size:
432 378 287 254 215
BsiEI CG_ry'CG
Cuts at: 0 1010 1098 1392 1566
Size: 1010 88 294 174
Fragments arranged by size:
1010 294 174 88
BsiHKAI G_wGCw'C
Cuts at: 0 644 1025 1566
Size: 644 381 541
Fragments arranged by size:
644 541 381
Bsp1286I G_dGCh'C
Cuts at: 0 36 71 194 283 537 644 683 915
Size: 36 35 123 89 254 107 39 232
Cuts at: 915 1025 1070 1104 1408 1416 1566
Size: 110 45 34 304 8 150
Fragments arranged by size:
304 254 232 150 123 110 107 89
45 39 36 35 34 8
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 67 226 396 794 1566
Size: 67 159 170 398 772
Fragments arranged by size:
772 398 170 159 67
BsrBI CCG'CTC
Cuts at: 0 1425 1566
Size: 1425 141
BsrDI GCAATG_nn'
Cuts at: 0 698 1566
Size: 698 868
BsrFI r'CCGG_y
Cuts at: 0 209 668 1566
Size: 209 459 898
Fragments arranged by size:
898 459 209
BssHII G'CGCG_C
Cuts at: 0 327 1566
Size: 327 1239
BstAPI GCAn_nnn'nTGC
Cuts at: 0 252 672 807 1065 1566
Size: 252 420 135 258 501
Fragments arranged by size:
501 420 258 252 135
BstYI r'GATC_y
Cuts at: 0 824 1566
Size: 824 742
Bsu36I CC'TnA_GG
Cuts at: 0 1417 1516 1566
Size: 1417 99 50
Fragments arranged by size:
1417 99 50
BtgI C'CryG_G
Cuts at: 0 10 385 1010 1566
Size: 10 375 625 556
Fragments arranged by size:
625 556 375 10
BtsI GCAGTG_nn'
Cuts at: 0 412 1566
Size: 412 1154
EaeI y'GGCC_r
Cuts at: 0 382 1007 1389 1566
Size: 382 625 382 177
Fragments arranged by size:
625 382 382 177
EagI C'GGCC_G
Cuts at: 0 1007 1389 1566
Size: 1007 382 177
Fragments arranged by size:
1007 382 177
EarI CTCTTCn'nnn_
Cuts at: 0 271 1566
Size: 271 1295
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 547 1566
Size: 547 1019
EcoICRI GAG'CTC
Cuts at: 0 1023 1566
Size: 1023 543
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 152 891 1039 1178 1498 1513 1566
Size: 152 739 148 139 320 15 53
Fragments arranged by size:
739 320 152 148 139 53 15
EcoNI CCTnn'n_nnAGG
Cuts at: 0 218 245 541 1566
Size: 218 27 296 1025
Fragments arranged by size:
1025 296 218 27
EcoO109I rG'GnC_Cy
Cuts at: 0 33 67 190 226 238 1039 1179 1256
Size: 33 34 123 36 12 801 140 77
Cuts at: 1256 1263 1289 1412 1413 1533 1552 1566
Size: 7 26 123 1 120 19 14
Fragments arranged by size:
801 140 123 123 120 77 36 34
33 26 19 14 12 7 1
FspI TGC'GCA
Cuts at: 0 63 1566
Size: 63 1503
HaeII r_GCGC'y
Cuts at: 0 352 1322 1566
Size: 352 970 244
Fragments arranged by size:
970 352 244
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 67 99 816 848 966 998 1176 1208
Size: 67 32 717 32 118 32 178 32
Cuts at: 1208 1566
Size: 358
Fragments arranged by size:
717 358 178 118 67 32 32 32
32
HincII GTy'rAC
Cuts at: 0 867 1566
Size: 867 699
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 198 1566
Size: 198 1368
MslI CAynn'nnrTG
Cuts at: 0 475 874 1009 1566
Size: 475 399 135 557
Fragments arranged by size:
557 475 399 135
MspA1I CmG'CkG
Cuts at: 0 12 258 1106 1152 1566
Size: 12 246 848 46 414
Fragments arranged by size:
848 414 246 46 12
PasI CC'CwG_GG
Cuts at: 0 283 1536 1566
Size: 283 1253 30
Fragments arranged by size:
1253 283 30
PflMI CCAn_nnn'nTGG
Cuts at: 0 200 372 517 636 657 690 711 1155
Size: 200 172 145 119 21 33 21 444
Cuts at: 1155 1347 1536 1566
Size: 192 189 30
Fragments arranged by size:
444 200 192 189 172 145 119 33
30 21 21
PmlI CAC'GTG
Cuts at: 0 477 633 1566
Size: 477 156 933
Fragments arranged by size:
933 477 156
PpuMI rG'GwC_Cy
Cuts at: 0 238 1039 1179 1256 1263 1289 1566
Size: 238 801 140 77 7 26 277
Fragments arranged by size:
801 277 238 140 77 26 7
PshAI GACnn'nnGTC
Cuts at: 0 425 1566
Size: 425 1141
PspOMI G'GGCC_C
Cuts at: 0 32 67 190 1412 1566
Size: 32 35 123 1222 154
Fragments arranged by size:
1222 154 123 35 32
PspXI vC'TCGA_Gb
Cuts at: 0 1407 1566
Size: 1407 159
PstI C_TGCA'G
Cuts at: 0 257 419 1566
Size: 257 162 1147
Fragments arranged by size:
1147 257 162
PvuII CAG'CTG
Cuts at: 0 1152 1566
Size: 1152 414
SacI G_AGCT'C
Cuts at: 0 1025 1566
Size: 1025 541
SacII CC_GC'GG
Cuts at: 0 13 1566
Size: 13 1553
SanDI GG'GwC_CC
Cuts at: 0 1039 1566
Size: 1039 527
SapI GCTCTTCn'nnn_
Cuts at: 0 271 1566
Size: 271 1295
SfcI C'TryA_G
Cuts at: 0 253 415 860 1566
Size: 253 162 445 706
Fragments arranged by size:
706 445 253 162
SmaI CCC'GGG
Cuts at: 0 31 72 1566
Size: 31 41 1494
Fragments arranged by size:
1494 41 31
SmlI C'TyrA_G
Cuts at: 0 423 817 1328 1407 1504 1566
Size: 423 394 511 79 97 62
Fragments arranged by size:
511 423 394 97 79 62
StuI AGG'CCT
Cuts at: 0 1436 1493 1566
Size: 1436 57 73
Fragments arranged by size:
1436 73 57
StyI C'CwwG_G
Cuts at: 0 1285 1431 1566
Size: 1285 146 135
Fragments arranged by size:
1285 146 135
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 57 108 1566
Size: 57 51 1458
Fragments arranged by size:
1458 57 51
TatI w'GTAC_w
Cuts at: 0 171 1112 1566
Size: 171 941 454
Fragments arranged by size:
941 454 171
XhoI C'TCGA_G
Cuts at: 0 1328 1407 1566
Size: 1328 79 159
Fragments arranged by size:
1328 159 79
XmaI C'CCGG_G
Cuts at: 0 29 70 1566
Size: 29 41 1496
Fragments arranged by size:
1496 41 29
Enzymes that do cut:
AcuI AflIII AgeI AleI AlwNI ApaI AvaI AvrII
BaeI BamHI BanI BanII BbvCI BciVI BglI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI
BsrBI BsrDI BsrFI BssHII BstAPI BstYI Bsu36I BtgI
BtsI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I FspI HaeII Hin4I HincII MmeI MslI MspA1I
PasI PflMI PmlI PpuMI PshAI PspOMI PspXI PstI
PvuII SacI SacII SanDI SapI SfcI SmaI SmlI
StuI StyI TaqII TatI XhoI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AhdI
AlfI AloI ApaLI ApoI AscI AseI AsiSI BbeI
BbsI BcgI BclI BfrBI BglII BlpI BmgBI BmtI
BsaBI BsaHI BsiWI BsmI BsmBI BspEI BspHI BsrGI
BssSI BstBI BstEII BstXI BstZ17I BtgZI ClaI CspCI
DraI DraIII DrdI EcoRI EcoRV FalI FseI FspAI
HindIII HpaI KasI KpnI MfeI MluI MscI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
NspI PacI PciI PfoI PmeI PpiI PsiI PsrI
PvuI RsrII SalI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI
TstI Tth111I XbaI XcmI XmnI ZraI