Restriction map for
pF1KB5260
(Linear) (Six-base) MAPSORT of: KIBB5260.seq Check: 9913 from: 1 to: 1143
KIBB5260.orf
With 182 enzymes: SgfI *
July 18, 2007 13:37 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 64 1143
Size: 64 1079
AhdI GACnn_n'nnGTC
Cuts at: 0 330 1143
Size: 330 813
AleI CACnn'nnGTG
Cuts at: 0 1015 1143
Size: 1015 128
ApaI G_GGCC'C
Cuts at: 0 1031 1082 1143
Size: 1031 51 61
Fragments arranged by size:
1031 61 51
AvaI C'yCGr_G
Cuts at: 0 1001 1022 1143
Size: 1001 21 121
Fragments arranged by size:
1001 121 21
BamHI G'GATC_C
Cuts at: 0 77 1143
Size: 77 1066
BanI G'GyrC_C
Cuts at: 0 622 892 947 1143
Size: 622 270 55 196
Fragments arranged by size:
622 270 196 55
BanII G_rGCy'C
Cuts at: 0 818 1031 1082 1143
Size: 818 213 51 61
Fragments arranged by size:
818 213 61 51
BbeI G_GCGC'C
Cuts at: 0 951 1143
Size: 951 192
BbvCI CC'TCA_GC
Cuts at: 0 718 748 1143
Size: 718 30 395
Fragments arranged by size:
718 395 30
BclI T'GATC_A
Cuts at: 0 531 1143
Size: 531 612
BglI GCCn_nnn'nGGC
Cuts at: 0 47 1143
Size: 47 1096
Bme1580I G_kGCm'C
Cuts at: 0 895 1031 1082 1143
Size: 895 136 51 61
Fragments arranged by size:
895 136 61 51
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 190 796 832 1143
Size: 190 606 36 311
Fragments arranged by size:
606 311 190 36
Bpu10I CC'TnA_GC
Cuts at: 0 718 748 1143
Size: 718 30 395
Fragments arranged by size:
718 395 30
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 646 1018 1081 1143
Size: 646 372 63 62
Fragments arranged by size:
646 372 63 62
BsaI GGTCTCn'nnnn_
Cuts at: 0 762 1143
Size: 762 381
BsaAI yAC'GTr
Cuts at: 0 63 555 936 1143
Size: 63 492 381 207
Fragments arranged by size:
492 381 207 63
BsaHI Gr'CG_yC
Cuts at: 0 326 948 1143
Size: 326 622 195
Fragments arranged by size:
622 326 195
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 554 584 1143
Size: 554 30 559
Fragments arranged by size:
559 554 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 43 253 305 334 450 754 943 1143
Size: 43 210 52 29 116 304 189 200
Fragments arranged by size:
304 210 200 189 116 52 43 29
BseYI C'CCAG_C
Cuts at: 0 478 1074 1143
Size: 478 596 69
Fragments arranged by size:
596 478 69
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 55 85 1143
Size: 55 30 1058
Fragments arranged by size:
1058 55 30
BsiEI CG_ry'CG
Cuts at: 0 641 681 1143
Size: 641 40 462
Fragments arranged by size:
641 462 40
BsiHKAI G_wGCw'C
Cuts at: 0 158 410 1143
Size: 158 252 733
Fragments arranged by size:
733 252 158
Bsp1286I G_dGCh'C
Cuts at: 0 158 410 818 895 1031 1082 1143
Size: 158 252 408 77 136 51 61
Fragments arranged by size:
408 252 158 136 77 61 51
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 75 1143
Size: 75 1068
BsrFI r'CCGG_y
Cuts at: 0 284 692 914 995 1052 1143
Size: 284 408 222 81 57 91
Fragments arranged by size:
408 284 222 91 81 57
BsrGI T'GTAC_A
Cuts at: 0 311 1143
Size: 311 832
BstEII G'GTnAC_C
Cuts at: 0 570 1143
Size: 570 573
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1017 1143
Size: 1017 126
BstYI r'GATC_y
Cuts at: 0 77 1091 1143
Size: 77 1014 52
Fragments arranged by size:
1014 77 52
BtgI C'CryG_G
Cuts at: 0 797 1143
Size: 797 346
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 459 1143
Size: 459 684
BtsI GCAGTG_nn'
Cuts at: 0 982 1143
Size: 982 161
DrdI GACnn_nn'nnGTC
Cuts at: 0 103 1143
Size: 103 1040
EaeI y'GGCC_r
Cuts at: 0 383 1007 1143
Size: 383 624 136
Fragments arranged by size:
624 383 136
EarI CTCTTCn'nnn_
Cuts at: 0 415 1143
Size: 415 728
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 817 1143
Size: 817 326
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 64 190 796 832 1143
Size: 64 126 606 36 311
Fragments arranged by size:
606 311 126 64 36
EcoNI CCTnn'n_nnAGG
Cuts at: 0 339 751 1143
Size: 339 412 392
Fragments arranged by size:
412 392 339
EcoO109I rG'GnC_Cy
Cuts at: 0 87 955 1027 1028 1078 1079 1143
Size: 87 868 72 1 50 1 64
Fragments arranged by size:
868 87 72 64 50 1 1
HaeII r_GCGC'y
Cuts at: 0 613 915 951 1143
Size: 613 302 36 192
Fragments arranged by size:
613 302 192 36
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 229 261 316 348 1143
Size: 229 32 55 32 795
Fragments arranged by size:
795 229 55 32 32
KasI G'GCGC_C
Cuts at: 0 947 1143
Size: 947 196
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 203 1143
Size: 203 940
MscI TGG'CCA
Cuts at: 0 385 1143
Size: 385 758
MslI CAynn'nnrTG
Cuts at: 0 422 703 1015 1143
Size: 422 281 312 128
Fragments arranged by size:
422 312 281 128
MspA1I CmG'CkG
Cuts at: 0 69 1143
Size: 69 1074
NaeI GCC'GGC
Cuts at: 0 286 1143
Size: 286 857
NarI GG'CG_CC
Cuts at: 0 948 1143
Size: 948 195
NgoMIV G'CCGG_C
Cuts at: 0 284 1143
Size: 284 859
PasI CC'CwG_GG
Cuts at: 0 629 1143
Size: 629 514
PfoI T'CCnGG_A
Cuts at: 0 149 1143
Size: 149 994
PpuMI rG'GwC_Cy
Cuts at: 0 87 955 1143
Size: 87 868 188
Fragments arranged by size:
868 188 87
PspOMI G'GGCC_C
Cuts at: 0 1027 1078 1143
Size: 1027 51 65
Fragments arranged by size:
1027 65 51
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 727 759 1143
Size: 727 32 384
Fragments arranged by size:
727 384 32
PstI C_TGCA'G
Cuts at: 0 911 1143
Size: 911 232
PvuII CAG'CTG
Cuts at: 0 69 1143
Size: 69 1074
SanDI GG'GwC_CC
Cuts at: 0 955 1143
Size: 955 188
SapI GCTCTTCn'nnn_
Cuts at: 0 415 1143
Size: 415 728
SfcI C'TryA_G
Cuts at: 0 907 1143
Size: 907 236
SfoI GGC'GCC
Cuts at: 0 949 1143
Size: 949 194
SgrAI Cr'CCGG_yG
Cuts at: 0 284 1143
Size: 284 859
SmaI CCC'GGG
Cuts at: 0 1003 1024 1143
Size: 1003 21 119
Fragments arranged by size:
1003 119 21
SmlI C'TyrA_G
Cuts at: 0 661 1033 1096 1143
Size: 661 372 63 47
Fragments arranged by size:
661 372 63 47
StyI C'CwwG_G
Cuts at: 0 356 386 1143
Size: 356 30 757
Fragments arranged by size:
757 356 30
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 558 1143
Size: 558 585
TatI w'GTAC_w
Cuts at: 0 311 1143
Size: 311 832
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 554 586 1143
Size: 554 32 557
Fragments arranged by size:
557 554 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 378 829 859 1143
Size: 378 451 30 284
Fragments arranged by size:
451 378 284 30
XmaI C'CCGG_G
Cuts at: 0 1001 1022 1143
Size: 1001 21 121
Fragments arranged by size:
1001 121 21
Enzymes that do cut:
AcuI AhdI AleI ApaI AvaI BamHI BanI BanII
BbeI BbvCI BclI BglI Bme1580I BpmI Bpu10I BpuEI
BsaI BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspMI BsrFI BsrGI BstEII BstXI BstYI
BtgI BtgZI BtsI DrdI EaeI EarI EciI Eco57MI
EcoNI EcoO109I HaeII Hin4I KasI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV PasI PfoI PpuMI PspOMI
PsrI PstI PvuII SanDI SapI SfcI SfoI SgrAI
SmaI SmlI StyI TaqII TatI TstI XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AlfI AloI AlwNI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BbsI BcgI BciVI BfrBI BglII
BlpI BmgBI BmrI BmtI BplI BsaBI BsaWI BsiWI
BsmI BsmBI BspEI BspHI BsrBI BsrDI BssHII BssSI
BstAPI BstBI BstZ17I Bsu36I ClaI CspCI DraI DraIII
EagI EcoICRI EcoRI EcoRV FalI FseI FspI FspAI
HincII HindIII HpaI KpnI MfeI MluI NcoI NdeI
NheI NotI NruI NsiI NspI PacI PciI PflMI
PmeI PmlI PpiI PshAI PsiI PspXI PvuI RsrII
SacI SacII SalI SbfI ScaI SexAI SfiI SgfI
SnaBI SpeI SphI SrfI SspI StuI SwaI Tth111I
XbaI XhoI XmnI ZraI