Restriction map for
pF1KB5263
(Linear) (Six-base) MAPSORT of: KIBB5263.seq Check: 2444 from: 1 to: 1137
KIBB5263.orf
With 182 enzymes: SgfI *
February 28, 2007 11:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 538 1137
Size: 538 599
AccI GT'mk_AC
Cuts at: 0 353 1137
Size: 353 784
Acc65I G'GTAC_C
Cuts at: 0 626 1137
Size: 626 511
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1099 1137
Size: 1099 38
AfeI AGC'GCT
Cuts at: 0 971 1137
Size: 971 166
AflII C'TTAA_G
Cuts at: 0 538 1137
Size: 538 599
AhdI GACnn_n'nnGTC
Cuts at: 0 684 1137
Size: 684 453
AlwNI CAG_nnn'CTG
Cuts at: 0 273 369 411 450 849 1137
Size: 273 96 42 39 399 288
Fragments arranged by size:
399 288 273 96 42 39
ApaI G_GGCC'C
Cuts at: 0 118 636 1137
Size: 118 518 501
Fragments arranged by size:
518 501 118
AvaI C'yCGr_G
Cuts at: 0 63 72 1137
Size: 63 9 1065
Fragments arranged by size:
1065 63 9
BanI G'GyrC_C
Cuts at: 0 626 1137
Size: 626 511
BanII G_rGCy'C
Cuts at: 0 41 118 636 787 1034 1088 1137
Size: 41 77 518 151 247 54 49
Fragments arranged by size:
518 247 151 77 54 49 41
BglII A'GATC_T
Cuts at: 0 495 1137
Size: 495 642
BlpI GC'TnA_GC
Cuts at: 0 412 1137
Size: 412 725
Bme1580I G_kGCm'C
Cuts at: 0 118 636 1137
Size: 118 518 501
Fragments arranged by size:
518 501 118
BmgBI CAC'GTC
Cuts at: 0 111 1137
Size: 111 1026
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 732 1137
Size: 732 405
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1009 1137
Size: 1009 128
Bpu10I CC'TnA_GC
Cuts at: 0 17 578 1137
Size: 17 561 559
Fragments arranged by size:
561 559 17
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 445 1137
Size: 445 692
BsaAI yAC'GTr
Cuts at: 0 186 1137
Size: 186 951
BsaBI GATnn'nnATC
Cuts at: 0 268 1137
Size: 268 869
BsaHI Gr'CG_yC
Cuts at: 0 325 1137
Size: 325 812
BsaWI w'CCGG_w
Cuts at: 0 923 1137
Size: 923 214
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 457 487 1137
Size: 457 30 650
Fragments arranged by size:
650 457 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1042 1137
Size: 1042 95
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 382 1137
Size: 382 755
BsiHKAI G_wGCw'C
Cuts at: 0 488 1088 1137
Size: 488 600 49
Fragments arranged by size:
600 488 49
BsiWI C'GTAC_G
Cuts at: 0 155 1137
Size: 155 982
BsmI GAATG_Cn'
Cuts at: 0 698 1137
Size: 698 439
Bsp1286I G_dGCh'C
Cuts at: 0 41 118 488 636 787 1034 1088 1137
Size: 41 77 370 148 151 247 54 49
Fragments arranged by size:
370 247 151 148 77 54 49 41
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 521 538 923 1137
Size: 521 17 385 214
Fragments arranged by size:
521 385 214 17
BsrBI CCG'CTC
Cuts at: 0 1074 1137
Size: 1074 63
BsrDI GCAATG_nn'
Cuts at: 0 423 1137
Size: 423 714
BsrFI r'CCGG_y
Cuts at: 0 592 722 1137
Size: 592 130 415
Fragments arranged by size:
592 415 130
BsrGI T'GTAC_A
Cuts at: 0 386 1137
Size: 386 751
BstBI TT'CG_AA
Cuts at: 0 227 1137
Size: 227 910
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1053 1137
Size: 1053 84
BstYI r'GATC_y
Cuts at: 0 263 269 446 495 1137
Size: 263 6 177 49 642
Fragments arranged by size:
642 263 177 49 6
Bsu36I CC'TnA_GG
Cuts at: 0 1067 1137
Size: 1067 70
BtgI C'CryG_G
Cuts at: 0 1046 1137
Size: 1046 91
EaeI y'GGCC_r
Cuts at: 0 203 1022 1137
Size: 203 819 115
Fragments arranged by size:
819 203 115
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 274 465 1137
Size: 274 191 672
Fragments arranged by size:
672 274 191
EcoICRI GAG'CTC
Cuts at: 0 1086 1137
Size: 1086 51
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1009 1099 1137
Size: 1009 90 38
Fragments arranged by size:
1009 90 38
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1066 1137
Size: 1066 71
EcoO109I rG'GnC_Cy
Cuts at: 0 69 366 456 633 801 1131 1137
Size: 69 297 90 177 168 330 6
Fragments arranged by size:
330 297 177 168 90 69 6
FspI TGC'GCA
Cuts at: 0 190 1137
Size: 190 947
HaeII r_GCGC'y
Cuts at: 0 973 1137
Size: 973 164
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 558 590 670 702 1137
Size: 558 32 80 32 435
Fragments arranged by size:
558 435 80 32 32
HincII GTy'rAC
Cuts at: 0 932 1137
Size: 932 205
HindIII A'AGCT_T
Cuts at: 0 880 1137
Size: 880 257
HpaI GTT'AAC
Cuts at: 0 932 1137
Size: 932 205
KpnI G_GTAC'C
Cuts at: 0 630 1137
Size: 630 507
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 533 1137
Size: 533 604
MscI TGG'CCA
Cuts at: 0 205 1137
Size: 205 932
MslI CAynn'nnrTG
Cuts at: 0 293 820 1137
Size: 293 527 317
Fragments arranged by size:
527 317 293
MspA1I CmG'CkG
Cuts at: 0 411 1137
Size: 411 726
NcoI C'CATG_G
Cuts at: 0 1046 1137
Size: 1046 91
PflMI CCAn_nnn'nTGG
Cuts at: 0 771 1137
Size: 771 366
PfoI T'CCnGG_A
Cuts at: 0 257 1094 1137
Size: 257 837 43
Fragments arranged by size:
837 257 43
PmlI CAC'GTG
Cuts at: 0 186 1137
Size: 186 951
PpuMI rG'GwC_Cy
Cuts at: 0 69 366 801 1137
Size: 69 297 435 336
Fragments arranged by size:
435 336 297 69
PspOMI G'GGCC_C
Cuts at: 0 114 632 1137
Size: 114 518 505
Fragments arranged by size:
518 505 114
PvuII CAG'CTG
Cuts at: 0 411 1137
Size: 411 726
SacI G_AGCT'C
Cuts at: 0 1088 1137
Size: 1088 49
SanDI GG'GwC_CC
Cuts at: 0 69 1137
Size: 69 1068
ScaI AGT'ACT
Cuts at: 0 997 1137
Size: 997 140
SfcI C'TryA_G
Cuts at: 0 841 1137
Size: 841 296
SmaI CCC'GGG
Cuts at: 0 65 74 1137
Size: 65 9 1063
Fragments arranged by size:
1063 65 9
SmlI C'TyrA_G
Cuts at: 0 460 538 1137
Size: 460 78 599
Fragments arranged by size:
599 460 78
StyI C'CwwG_G
Cuts at: 0 656 863 1046 1137
Size: 656 207 183 91
Fragments arranged by size:
656 207 183 91
TatI w'GTAC_w
Cuts at: 0 137 386 464 995 1137
Size: 137 249 78 531 142
Fragments arranged by size:
531 249 142 137 78
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 864 1137
Size: 864 273
XmaI C'CCGG_G
Cuts at: 0 63 72 1137
Size: 63 9 1065
Fragments arranged by size:
1065 63 9
Enzymes that do cut:
AarI AccI Acc65I AcuI AfeI AflII AhdI AlwNI
ApaI AvaI BanI BanII BglII BlpI Bme1580I BmgBI
BplI BpmI Bpu10I BpuEI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BsgI BsiHKAI BsiWI BsmI Bsp1286I BspMI
BsrBI BsrDI BsrFI BsrGI BstBI BstXI BstYI Bsu36I
BtgI EaeI EciI EcoICRI Eco57MI EcoNI EcoO109I FspI
HaeII Hin4I HincII HindIII HpaI KpnI MmeI MscI
MslI MspA1I NcoI PflMI PfoI PmlI PpuMI PspOMI
PvuII SacI SanDI ScaI SfcI SmaI SmlI StyI
TatI XcmI XmaI
Enzymes that do not cut:
AatII AclI AflIII AgeI AleI AlfI AloI ApaLI
ApoI AscI AseI AsiSI AvrII BaeI BamHI BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BmrI
BmtI BsaI BseYI BsiEI BsmBI BspEI BspHI BssHII
BssSI BstAPI BstEII BstZ17I BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EagI EarI EcoRI EcoRV FalI
FseI FspAI KasI MfeI MluI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI NspI PacI PasI
PciI PmeI PpiI PshAI PsiI PspXI PsrI PstI
PvuI RsrII SacII SalI SapI SbfI SexAI SfiI
SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SspI
StuI SwaI TaqII TstI Tth111I XbaI XhoI XmnI
ZraI