Restriction map for
pF1KB5268
(Linear) (Six-base) MAPSORT of: KIBB5268.seq Check: 2939 from: 1 to: 1104
KIBB5268.orf
With 182 enzymes: SgfI *
September 18, 2009 17:00 ..
AfeI AGC'GCT
Cuts at: 0 731 1104
Size: 731 373
AleI CACnn'nnGTG
Cuts at: 0 403 1104
Size: 403 701
ApaI G_GGCC'C
Cuts at: 0 253 512 531 1009 1104
Size: 253 259 19 478 95
Fragments arranged by size:
478 259 253 95 19
ApaLI G'TGCA_C
Cuts at: 0 352 920 1104
Size: 352 568 184
Fragments arranged by size:
568 352 184
AvaI C'yCGr_G
Cuts at: 0 226 310 505 703 1045 1104
Size: 226 84 195 198 342 59
Fragments arranged by size:
342 226 198 195 84 59
BanI G'GyrC_C
Cuts at: 0 568 786 962 1104
Size: 568 218 176 142
Fragments arranged by size:
568 218 176 142
BanII G_rGCy'C
Cuts at: 0 253 512 531 1009 1069 1104
Size: 253 259 19 478 60 35
Fragments arranged by size:
478 259 253 60 35 19
BbeI G_GCGC'C
Cuts at: 0 572 790 1104
Size: 572 218 314
Fragments arranged by size:
572 314 218
BglI GCCn_nnn'nGGC
Cuts at: 0 228 409 516 535 621 690 817 841
Size: 228 181 107 19 86 69 127 24
Cuts at: 841 850 1104
Size: 9 254
Fragments arranged by size:
254 228 181 127 107 86 69 24
19 9
Bme1580I G_kGCm'C
Cuts at: 0 84 253 356 512 531 924 975 1009
Size: 84 169 103 156 19 393 51 34
Cuts at: 1009 1104
Size: 95
Fragments arranged by size:
393 169 156 103 95 84 51 34
19
BmrI ACTGGGnnnn_n'
Cuts at: 0 910 1104
Size: 910 194
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 973 1016 1104
Size: 973 43 88
Fragments arranged by size:
973 88 43
BsaI GGTCTCn'nnnn_
Cuts at: 0 987 1104
Size: 987 117
BsaHI Gr'CG_yC
Cuts at: 0 569 787 1104
Size: 569 218 317
Fragments arranged by size:
569 317 218
BseYI C'CCAG_C
Cuts at: 0 87 201 231 426 496 726 1104
Size: 87 114 30 195 70 230 378
Fragments arranged by size:
378 230 195 114 87 70 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 288 930 1104
Size: 288 642 174
Fragments arranged by size:
642 288 174
BsiEI CG_ry'CG
Cuts at: 0 185 379 520 625 1104
Size: 185 194 141 105 479
Fragments arranged by size:
479 194 185 141 105
BsiHKAI G_wGCw'C
Cuts at: 0 356 392 398 924 1104
Size: 356 36 6 526 180
Fragments arranged by size:
526 356 180 36 6
Bsp1286I G_dGCh'C
Cuts at: 0 84 253 356 392 398 512 531 924
Size: 84 169 103 36 6 114 19 393
Cuts at: 924 975 1009 1069 1104
Size: 51 34 60 35
Fragments arranged by size:
393 169 114 103 84 60 51 36
35 34 19 6
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 182 1104
Size: 182 922
BsrBI CCG'CTC
Cuts at: 0 271 289 522 647 687 1104
Size: 271 18 233 125 40 417
Fragments arranged by size:
417 271 233 125 40 18
BsrDI GCAATG_nn'
Cuts at: 0 988 1104
Size: 988 116
BsrFI r'CCGG_y
Cuts at: 0 297 624 1104
Size: 297 327 480
Fragments arranged by size:
480 327 297
BssHII G'CGCG_C
Cuts at: 0 98 431 628 1104
Size: 98 333 197 476
Fragments arranged by size:
476 333 197 98
BssSI C'ACGA_G
Cuts at: 0 391 1104
Size: 391 713
BstAPI GCAn_nnn'nTGC
Cuts at: 0 360 1104
Size: 360 744
Bsu36I CC'TnA_GG
Cuts at: 0 610 1104
Size: 610 494
BtgI C'CryG_G
Cuts at: 0 413 619 737 803 967 1104
Size: 413 206 118 66 164 137
Fragments arranged by size:
413 206 164 137 118 66
BtsI GCAGTG_nn'
Cuts at: 0 82 922 1104
Size: 82 840 182
Fragments arranged by size:
840 182 82
EaeI y'GGCC_r
Cuts at: 0 2 182 212 317 364 376 517 622
Size: 2 180 30 105 47 12 141 105
Cuts at: 622 734 1104
Size: 112 370
Fragments arranged by size:
370 180 141 112 105 105 47 30
12 2
EagI C'GGCC_G
Cuts at: 0 182 376 517 622 1104
Size: 182 194 141 105 482
Fragments arranged by size:
482 194 182 141 105
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 273 1077 1104
Size: 273 804 27
Fragments arranged by size:
804 273 27
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 973 1016 1104
Size: 973 43 88
Fragments arranged by size:
973 88 43
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1013 1104
Size: 1013 91
EcoO109I rG'GnC_Cy
Cuts at: 0 250 501 509 528 614 635 1005 1006
Size: 250 251 8 19 86 21 370 1
Cuts at: 1006 1018 1104
Size: 12 86
Fragments arranged by size:
370 251 250 86 86 21 19 12
8 1
FspI TGC'GCA
Cuts at: 0 121 1104
Size: 121 983
HaeII r_GCGC'y
Cuts at: 0 572 733 790 885 1104
Size: 572 161 57 95 219
Fragments arranged by size:
572 219 161 95 57
KasI G'GCGC_C
Cuts at: 0 568 786 1104
Size: 568 218 318
Fragments arranged by size:
568 318 218
MscI TGG'CCA
Cuts at: 0 4 1104
Size: 4 1100
MslI CAynn'nnrTG
Cuts at: 0 403 586 1104
Size: 403 183 518
Fragments arranged by size:
518 403 183
MspA1I CmG'CkG
Cuts at: 0 64 201 360 415 805 969 1104
Size: 64 137 159 55 390 164 135
Fragments arranged by size:
390 164 159 137 135 64 55
NaeI GCC'GGC
Cuts at: 0 299 626 1104
Size: 299 327 478
Fragments arranged by size:
478 327 299
NarI GG'CG_CC
Cuts at: 0 569 787 1104
Size: 569 218 317
Fragments arranged by size:
569 317 218
NgoMIV G'CCGG_C
Cuts at: 0 297 624 1104
Size: 297 327 480
Fragments arranged by size:
480 327 297
NotI GC'GGCC_GC
Cuts at: 0 182 376 1104
Size: 182 194 728
Fragments arranged by size:
728 194 182
PpuMI rG'GwC_Cy
Cuts at: 0 635 1104
Size: 635 469
PspOMI G'GGCC_C
Cuts at: 0 249 508 527 1005 1104
Size: 249 259 19 478 99
Fragments arranged by size:
478 259 249 99 19
PstI C_TGCA'G
Cuts at: 0 881 1104
Size: 881 223
PvuII CAG'CTG
Cuts at: 0 64 1104
Size: 64 1040
SacII CC_GC'GG
Cuts at: 0 416 806 970 1104
Size: 416 390 164 134
Fragments arranged by size:
416 390 164 134
SanDI GG'GwC_CC
Cuts at: 0 635 1104
Size: 635 469
SfcI C'TryA_G
Cuts at: 0 877 1104
Size: 877 227
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 516 535 621 1104
Size: 516 19 86 483
Fragments arranged by size:
516 483 86 19
SfoI GGC'GCC
Cuts at: 0 570 788 1104
Size: 570 218 316
Fragments arranged by size:
570 316 218
SmaI CCC'GGG
Cuts at: 0 228 705 1104
Size: 228 477 399
Fragments arranged by size:
477 399 228
SrfI GCCC'GGGC
Cuts at: 0 228 1104
Size: 228 876
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 477 1104
Size: 477 627
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 198 1104
Size: 198 906
XmaI C'CCGG_G
Cuts at: 0 226 703 1104
Size: 226 477 401
Fragments arranged by size:
477 401 226
Enzymes that do cut:
AfeI AleI ApaI ApaLI AvaI BanI BanII BbeI
BglI Bme1580I BmrI BpmI BsaI BsaHI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspMI BsrBI BsrDI BsrFI BssHII
BssSI BstAPI Bsu36I BtgI BtsI EaeI EagI EciI
Eco57MI EcoNI EcoO109I FspI HaeII KasI MscI MslI
MspA1I NaeI NarI NgoMIV NotI PpuMI PspOMI PstI
PvuII SacII SanDI SfcI SfiI SfoI SmaI SrfI
TaqII XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AlfI AloI AlwNI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BbsI BbvCI BcgI BciVI
BclI BfrBI BglII BlpI BmgBI BmtI BplI Bpu10I
BpuEI BsaAI BsaBI BsaWI BsaXI BseRI BsiWI BsmI
BsmBI BspEI BspHI BsrGI BstBI BstEII BstXI BstYI
BstZ17I BtgZI ClaI CspCI DraI DraIII DrdI EarI
EcoICRI EcoRI EcoRV FalI FseI FspAI Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI NcoI NdeI
NheI NruI NsiI NspI PacI PasI PciI PflMI
PfoI PmeI PmlI PpiI PshAI PsiI PspXI PsrI
PvuI RsrII SacI SalI SapI SbfI ScaI SexAI
SgfI SgrAI SmlI SnaBI SpeI SphI SspI StuI
StyI SwaI TatI TstI Tth111I XbaI XhoI XmnI
ZraI