Restriction map for
pF1KB5271
(Linear) (Six-base) MAPSORT of: KIBB5271.seq Check: 4346 from: 1 to: 1251
KIBB5271.orf
With 182 enzymes: SgfI *
April 24, 2009 13:04 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1047 1251
Size: 1047 204
AccI GT'mk_AC
Cuts at: 0 1149 1251
Size: 1149 102
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 285 679 1251
Size: 285 394 572
Fragments arranged by size:
572 394 285
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 854 1251
Size: 854 397
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 452 484 1251
Size: 452 32 767
Fragments arranged by size:
767 452 32
ApoI r'AATT_y
Cuts at: 0 208 688 803 903 1025 1251
Size: 208 480 115 100 122 226
Fragments arranged by size:
480 226 208 122 115 100
AseI AT'TA_AT
Cuts at: 0 398 1251
Size: 398 853
AvrII C'CTAG_G
Cuts at: 0 495 1251
Size: 495 756
BbsI GAAGACnn'nnnn_
Cuts at: 0 43 1251
Size: 43 1208
BmrI ACTGGGnnnn_n'
Cuts at: 0 460 1251
Size: 460 791
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 328 735 1251
Size: 328 407 516
Fragments arranged by size:
516 407 328
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 110 1251
Size: 110 1141
Bpu10I CC'TnA_GC
Cuts at: 0 146 1251
Size: 146 1105
BsaI GGTCTCn'nnnn_
Cuts at: 0 248 1251
Size: 248 1003
BsaWI w'CCGG_w
Cuts at: 0 606 1251
Size: 606 645
BseYI C'CCAG_C
Cuts at: 0 136 1251
Size: 136 1115
BsiEI CG_ry'CG
Cuts at: 0 47 1251
Size: 47 1204
BsiHKAI G_wGCw'C
Cuts at: 0 489 1251
Size: 489 762
Bsp1286I G_dGCh'C
Cuts at: 0 489 1251
Size: 489 762
BspEI T'CCGG_A
Cuts at: 0 606 1251
Size: 606 645
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1047 1251
Size: 1047 204
BsrBI CCG'CTC
Cuts at: 0 6 26 1251
Size: 6 20 1225
Fragments arranged by size:
1225 20 6
BssSI C'ACGA_G
Cuts at: 0 317 1251
Size: 317 934
BstBI TT'CG_AA
Cuts at: 0 1023 1251
Size: 1023 228
BstZ17I GTA'TAC
Cuts at: 0 1150 1251
Size: 1150 101
BtgI C'CryG_G
Cuts at: 0 407 1251
Size: 407 844
EarI CTCTTCn'nnn_
Cuts at: 0 386 515 821 1222 1251
Size: 386 129 306 401 29
Fragments arranged by size:
401 386 306 129 29
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 110 285 679 1251
Size: 110 175 394 572
Fragments arranged by size:
572 394 175 110
EcoNI CCTnn'n_nnAGG
Cuts at: 0 61 1251
Size: 61 1190
EcoO109I rG'GnC_Cy
Cuts at: 0 15 1251
Size: 15 1236
EcoRI G'AATT_C
Cuts at: 0 208 1025 1251
Size: 208 817 226
Fragments arranged by size:
817 226 208
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1117 1251
Size: 1117 134
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 300 331 332 363 659 691 1251
Size: 300 31 1 31 296 32 560
Fragments arranged by size:
560 300 296 32 31 31 1
HincII GTy'rAC
Cuts at: 0 555 738 1251
Size: 555 183 513
Fragments arranged by size:
555 513 183
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 503 1251
Size: 503 748
MslI CAynn'nnrTG
Cuts at: 0 928 1251
Size: 928 323
NcoI C'CATG_G
Cuts at: 0 407 1251
Size: 407 844
PflMI CCAn_nnn'nTGG
Cuts at: 0 751 1251
Size: 751 500
PshAI GACnn'nnGTC
Cuts at: 0 43 1251
Size: 43 1208
PsiI TTA'TAA
Cuts at: 0 1133 1251
Size: 1133 118
SapI GCTCTTCn'nnn_
Cuts at: 0 386 515 821 1251
Size: 386 129 306 430
Fragments arranged by size:
430 386 306 129
SspI AAT'ATT
Cuts at: 0 334 696 851 1129 1251
Size: 334 362 155 278 122
Fragments arranged by size:
362 334 278 155 122
StuI AGG'CCT
Cuts at: 0 67 1251
Size: 67 1184
StyI C'CwwG_G
Cuts at: 0 407 495 774 1251
Size: 407 88 279 477
Fragments arranged by size:
477 407 279 88
TatI w'GTAC_w
Cuts at: 0 547 1251
Size: 547 704
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 849 1251
Size: 849 402
XmnI GAAnn'nnTTC
Cuts at: 0 399 1251
Size: 399 852
Enzymes that do cut:
AarI AccI AcuI AlfI AloI ApoI AseI AvrII
BbsI BmrI BplI BpmI Bpu10I BsaI BsaWI BseYI
BsiEI BsiHKAI Bsp1286I BspEI BspMI BsrBI BssSI BstBI
BstZ17I BtgI EarI Eco57MI EcoNI EcoO109I EcoRI FalI
Hin4I HincII MmeI MslI NcoI PflMI PshAI PsiI
SapI SspI StuI StyI TatI XcmI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AleI AlwNI ApaI ApaLI AscI AsiSI AvaI BaeI
BamHI BanI BanII BbeI BbvCI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmtI BpuEI
BsaAI BsaBI BsaHI BsaXI BseRI BsgI BsiWI BsmI
BsmBI BspHI BsrDI BsrFI BsrGI BssHII BstAPI BstEII
BstXI BstYI Bsu36I BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EciI EcoICRI EcoRV FseI
FspI FspAI HaeII HindIII HpaI KasI KpnI MfeI
MluI MscI MspA1I NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspI PacI PasI PciI PfoI
PmeI PmlI PpiI PpuMI PspOMI PspXI PsrI PstI
PvuI PvuII RsrII SacI SacII SalI SanDI SbfI
ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SwaI TaqII TstI
Tth111I XbaI XhoI XmaI ZraI