(Linear) (Six-base) MAPSORT of: KIBB5273.seq  Check: 6048  from: 1  to: 396



KIBB5273.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:15  ..

ApaLI G'TGCA_C

Cuts at:      0     372     396 
   Size:        372      24
 
ApoI r'AATT_y

Cuts at:      0      10     102     208     396 
   Size:         10      92     106     188

  Fragments arranged by size: 

                 188     106      92      10
 
BanI G'GyrC_C

Cuts at:      0      19     396 
   Size:         19     377
 
BclI T'GATC_A

Cuts at:      0     122     396 
   Size:        122     274
 
Bme1580I G_kGCm'C

Cuts at:      0      22     376     396 
   Size:         22     354      20

  Fragments arranged by size: 

                 354      22      20
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     106     396 
   Size:        106     290
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     304     396 
   Size:        304      92
 
BsiHKAI G_wGCw'C

Cuts at:      0     376     396 
   Size:        376      20
 
Bsp1286I G_dGCh'C

Cuts at:      0      22     376     396 
   Size:         22     354      20

  Fragments arranged by size: 

                 354      22      20
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      96     396 
   Size:         96     300
 
DraI TTT'AAA

Cuts at:      0     174     396 
   Size:        174     222
 
EaeI y'GGCC_r

Cuts at:      0     107     396 
   Size:        107     289
 
MscI TGG'CCA

Cuts at:      0     109     396 
   Size:        109     287
 
MspA1I CmG'CkG

Cuts at:      0     226     396 
   Size:        226     170
 
PsiI TTA'TAA

Cuts at:      0     148     396 
   Size:        148     248
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     150     182     396 
   Size:        150      32     214

  Fragments arranged by size: 

                 214     150      32
 
PstI C_TGCA'G

Cuts at:      0     263     396 
   Size:        263     133
 
PvuII CAG'CTG

Cuts at:      0     226     396 
   Size:        226     170
 
SfcI C'TryA_G

Cuts at:      0     259     396 
   Size:        259     137
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      61     396 
   Size:         61     335
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      93     396 
   Size:         93     303
 

 Enzymes that do cut:

    ApaLI     ApoI     BanI     BclI Bme1580I     BmrI     BsgI  BsiHKAI
 Bsp1286I    BstXI     DraI     EaeI     MscI   MspA1I     PsiI     PsrI
     PstI    PvuII     SfcI    TaqII     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI    BanII
     BbeI     BbsI    BbvCI     BcgI    BciVI    BfrBI     BglI    BglII
     BlpI    BmgBI     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI    BsiEI
    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstYI
  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI   DraIII
     DrdI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII    Hin4I
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI
     MslI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PpuMI    PshAI   PspOMI    PspXI     PvuI
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     StyI     SwaI     TatI
     TstI  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI