Restriction map for
pF1KB5275
(Linear) (Six-base) MAPSORT of: KIBB5275.seq Check: 1916 from: 1 to: 1407
KIBB5275.orf
With 182 enzymes: SgfI *
March 3, 2008 10:57 ..
AccI GT'mk_AC
Cuts at: 0 1202 1407
Size: 1202 205
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1275 1407
Size: 1275 132
AflIII A'CryG_T
Cuts at: 0 446 1407
Size: 446 961
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 664 1407
Size: 664 743
AlwNI CAG_nnn'CTG
Cuts at: 0 651 1135 1407
Size: 651 484 272
Fragments arranged by size:
651 484 272
ApaI G_GGCC'C
Cuts at: 0 30 207 1405 1407
Size: 30 177 1198 2
Fragments arranged by size:
1198 177 30 2
AvaI C'yCGr_G
Cuts at: 0 74 716 1356 1407
Size: 74 642 640 51
Fragments arranged by size:
642 640 74 51
BanI G'GyrC_C
Cuts at: 0 78 436 640 1407
Size: 78 358 204 767
Fragments arranged by size:
767 358 204 78
BanII G_rGCy'C
Cuts at: 0 30 207 1405 1407
Size: 30 177 1198 2
Fragments arranged by size:
1198 177 30 2
BbvCI CC'TCA_GC
Cuts at: 0 742 1407
Size: 742 665
BglI GCCn_nnn'nGGC
Cuts at: 0 25 614 638 1407
Size: 25 589 24 769
Fragments arranged by size:
769 589 25 24
BlpI GC'TnA_GC
Cuts at: 0 856 1407
Size: 856 551
Bme1580I G_kGCm'C
Cuts at: 0 30 207 286 643 872 1195 1405 1407
Size: 30 177 79 357 229 323 210 2
Fragments arranged by size:
357 323 229 210 177 79 30 2
BmgBI CAC'GTC
Cuts at: 0 447 711 879 1407
Size: 447 264 168 528
Fragments arranged by size:
528 447 264 168
BmrI ACTGGGnnnn_n'
Cuts at: 0 225 1407
Size: 225 1182
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 214 958 1114 1407
Size: 214 744 156 293
Fragments arranged by size:
744 293 214 156
Bpu10I CC'TnA_GC
Cuts at: 0 742 1407
Size: 742 665
BsaI GGTCTCn'nnnn_
Cuts at: 0 177 1407
Size: 177 1230
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 85 115 218 248 1407
Size: 85 30 103 30 1159
Fragments arranged by size:
1159 103 85 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 93 277 352 353 730 1407
Size: 93 184 75 1 377 677
Fragments arranged by size:
677 377 184 93 75 1
BseYI C'CCAG_C
Cuts at: 0 206 356 636 644 920 1222 1398 1407
Size: 206 150 280 8 276 302 176 9
Fragments arranged by size:
302 280 276 206 176 150 9 8
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 717 1407
Size: 717 690
BsiHKAI G_wGCw'C
Cuts at: 0 344 1407
Size: 344 1063
BsmBI CGTCTCn'nnnn_
Cuts at: 0 491 717 1407
Size: 491 226 690
Fragments arranged by size:
690 491 226
Bsp1286I G_dGCh'C
Cuts at: 0 30 207 286 344 643 872 1195 1405
Size: 30 177 79 58 299 229 323 210
Cuts at: 1405 1407
Size: 2
Fragments arranged by size:
323 299 229 210 177 79 58 30
2
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 356 1331 1407
Size: 356 975 76
Fragments arranged by size:
975 356 76
BsrFI r'CCGG_y
Cuts at: 0 140 1407
Size: 140 1267
BstAPI GCAn_nnn'nTGC
Cuts at: 0 348 1407
Size: 348 1059
BstEII G'GTnAC_C
Cuts at: 0 981 1407
Size: 981 426
BstYI r'GATC_y
Cuts at: 0 842 1088 1407
Size: 842 246 319
Fragments arranged by size:
842 319 246
Bsu36I CC'TnA_GG
Cuts at: 0 260 1253 1407
Size: 260 993 154
Fragments arranged by size:
993 260 154
BtgI C'CryG_G
Cuts at: 0 872 1407
Size: 872 535
BtsI GCAGTG_nn'
Cuts at: 0 228 700 956 1407
Size: 228 472 256 451
Fragments arranged by size:
472 451 256 228
DrdI GACnn_nn'nnGTC
Cuts at: 0 397 1407
Size: 397 1010
EaeI y'GGCC_r
Cuts at: 0 953 1407
Size: 953 454
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 214 958 1114 1275 1407
Size: 214 744 156 161 132
Fragments arranged by size:
744 214 161 156 132
EcoNI CCTnn'n_nnAGG
Cuts at: 0 325 1407
Size: 325 1082
EcoO109I rG'GnC_Cy
Cuts at: 0 26 1249 1402 1407
Size: 26 1223 153 5
Fragments arranged by size:
1223 153 26 5
FspI TGC'GCA
Cuts at: 0 1327 1407
Size: 1327 80
HaeII r_GCGC'y
Cuts at: 0 704 863 1407
Size: 704 159 544
Fragments arranged by size:
704 544 159
MscI TGG'CCA
Cuts at: 0 955 1407
Size: 955 452
MslI CAynn'nnrTG
Cuts at: 0 158 178 386 445 1407
Size: 158 20 208 59 962
Fragments arranged by size:
962 208 158 59 20
MspA1I CmG'CkG
Cuts at: 0 306 648 855 951 978 1407
Size: 306 342 207 96 27 429
Fragments arranged by size:
429 342 306 207 96 27
NaeI GCC'GGC
Cuts at: 0 142 1407
Size: 142 1265
NgoMIV G'CCGG_C
Cuts at: 0 140 1407
Size: 140 1267
NspI r_CATG'y
Cuts at: 0 945 1201 1407
Size: 945 256 206
Fragments arranged by size:
945 256 206
PflMI CCAn_nnn'nTGG
Cuts at: 0 651 1407
Size: 651 756
PfoI T'CCnGG_A
Cuts at: 0 191 1091 1407
Size: 191 900 316
Fragments arranged by size:
900 316 191
PpuMI rG'GwC_Cy
Cuts at: 0 1249 1407
Size: 1249 158
PspOMI G'GGCC_C
Cuts at: 0 26 203 1401 1407
Size: 26 177 1198 6
Fragments arranged by size:
1198 177 26 6
PstI C_TGCA'G
Cuts at: 0 257 311 419 854 1407
Size: 257 54 108 435 553
Fragments arranged by size:
553 435 257 108 54
PvuII CAG'CTG
Cuts at: 0 648 855 978 1407
Size: 648 207 123 429
Fragments arranged by size:
648 429 207 123
SfcI C'TryA_G
Cuts at: 0 253 307 415 850 1407
Size: 253 54 108 435 557
Fragments arranged by size:
557 435 253 108 54
SmaI CCC'GGG
Cuts at: 0 76 718 1407
Size: 76 642 689
Fragments arranged by size:
689 642 76
StuI AGG'CCT
Cuts at: 0 1391 1407
Size: 1391 16
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 398 601 658 769 1407
Size: 398 203 57 111 638
Fragments arranged by size:
638 398 203 111 57
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 98 130 218 250 1028 1060 1407
Size: 98 32 88 32 778 32 347
Fragments arranged by size:
778 347 98 88 32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 975 1407
Size: 975 432
XmaI C'CCGG_G
Cuts at: 0 74 716 1407
Size: 74 642 691
Fragments arranged by size:
691 642 74
Enzymes that do cut:
AccI AcuI AflIII AlfI AlwNI ApaI AvaI BanI
BanII BbvCI BglI BlpI Bme1580I BmgBI BmrI BpmI
Bpu10I BsaI BsaXI BseRI BseYI BsgI BsiHKAI BsmBI
Bsp1286I BspMI BsrFI BstAPI BstEII BstYI Bsu36I BtgI
BtsI DrdI EaeI Eco57MI EcoNI EcoO109I FspI HaeII
MscI MslI MspA1I NaeI NgoMIV NspI PflMI PfoI
PpuMI PspOMI PstI PvuII SfcI SmaI StuI TaqII
TstI XcmI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AleI AloI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BbsI BcgI BciVI BclI BfrBI
BglII BmtI BplI BpuEI BsaAI BsaBI BsaHI BsaWI
BsiEI BsiWI BsmI BspEI BspHI BsrBI BsrDI BsrGI
BssHII BssSI BstBI BstXI BstZ17I BtgZI ClaI CspCI
DraI DraIII EagI EarI EciI EcoICRI EcoRI EcoRV
FalI FseI FspAI Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI NarI NcoI NdeI NheI
NotI NruI NsiI PacI PasI PciI PmeI PmlI
PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI
SspI StyI SwaI TatI Tth111I XbaI XhoI XmnI
ZraI