Restriction map for
pF1KB5278
(Linear) (Six-base) MAPSORT of: KIBB5278.seq Check: 3479 from: 1 to: 1125
KIBB5278.orf
With 182 enzymes: SgfI *
October 7, 2009 14:15 ..
AccI GT'mk_AC
Cuts at: 0 728 1125
Size: 728 397
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 257 1125
Size: 257 868
AleI CACnn'nnGTG
Cuts at: 0 781 938 1125
Size: 781 157 187
Fragments arranged by size:
781 187 157
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 46 1125
Size: 46 1079
ApoI r'AATT_y
Cuts at: 0 561 1125
Size: 561 564
AvaI C'yCGr_G
Cuts at: 0 628 779 1125
Size: 628 151 346
Fragments arranged by size:
628 346 151
BanI G'GyrC_C
Cuts at: 0 634 1125
Size: 634 491
BanII G_rGCy'C
Cuts at: 0 797 1125
Size: 797 328
BbeI G_GCGC'C
Cuts at: 0 638 1125
Size: 638 487
BclI T'GATC_A
Cuts at: 0 404 1125
Size: 404 721
BglI GCCn_nnn'nGGC
Cuts at: 0 881 1125
Size: 881 244
Bme1580I G_kGCm'C
Cuts at: 0 788 1125
Size: 788 337
BmgBI CAC'GTC
Cuts at: 0 594 1125
Size: 594 531
BmrI ACTGGGnnnn_n'
Cuts at: 0 67 1125
Size: 67 1058
BmtI G_CTAG'C
Cuts at: 0 37 1125
Size: 37 1088
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 129 1125
Size: 129 996
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 82 1125
Size: 82 1043
Bpu10I CC'TnA_GC
Cuts at: 0 650 1125
Size: 650 475
BsaI GGTCTCn'nnnn_
Cuts at: 0 1003 1125
Size: 1003 122
BsaBI GATnn'nnATC
Cuts at: 0 808 1125
Size: 808 317
BsaHI Gr'CG_yC
Cuts at: 0 635 1125
Size: 635 490
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 80 110 1125
Size: 80 30 1015
Fragments arranged by size:
1015 80 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 121 1125
Size: 121 1004
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 982 1125
Size: 982 143
BsiHKAI G_wGCw'C
Cuts at: 0 982 1125
Size: 982 143
Bsp1286I G_dGCh'C
Cuts at: 0 788 797 982 1125
Size: 788 9 185 143
Fragments arranged by size:
788 185 143 9
BsrDI GCAATG_nn'
Cuts at: 0 498 1125
Size: 498 627
BsrFI r'CCGG_y
Cuts at: 0 685 1125
Size: 685 440
BstBI TT'CG_AA
Cuts at: 0 807 1125
Size: 807 318
BstXI CCAn_nnnn'nTGG
Cuts at: 0 123 327 1125
Size: 123 204 798
Fragments arranged by size:
798 204 123
BtgI C'CryG_G
Cuts at: 0 923 1125
Size: 923 202
BtsI GCAGTG_nn'
Cuts at: 0 610 915 923 1122 1125
Size: 610 305 8 199 3
Fragments arranged by size:
610 305 199 8 3
EaeI y'GGCC_r
Cuts at: 0 287 773 1125
Size: 287 486 352
Fragments arranged by size:
486 352 287
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 82 257 1125
Size: 82 175 868
Fragments arranged by size:
868 175 82
EcoRI G'AATT_C
Cuts at: 0 561 1125
Size: 561 564
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 108 1125
Size: 108 1017
FspI TGC'GCA
Cuts at: 0 22 616 1125
Size: 22 594 509
Fragments arranged by size:
594 509 22
HaeII r_GCGC'y
Cuts at: 0 638 1125
Size: 638 487
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 375 407 1125
Size: 375 32 718
Fragments arranged by size:
718 375 32
KasI G'GCGC_C
Cuts at: 0 634 1125
Size: 634 491
MscI TGG'CCA
Cuts at: 0 775 1125
Size: 775 350
MslI CAynn'nnrTG
Cuts at: 0 484 550 781 938 1004 1125
Size: 484 66 231 157 66 121
Fragments arranged by size:
484 231 157 121 66 66
MspA1I CmG'CkG
Cuts at: 0 145 467 476 645 1125
Size: 145 322 9 169 480
Fragments arranged by size:
480 322 169 145 9
NaeI GCC'GGC
Cuts at: 0 687 1125
Size: 687 438
NarI GG'CG_CC
Cuts at: 0 635 1125
Size: 635 490
NcoI C'CATG_G
Cuts at: 0 923 1125
Size: 923 202
NgoMIV G'CCGG_C
Cuts at: 0 685 1125
Size: 685 440
NheI G'CTAG_C
Cuts at: 0 33 1125
Size: 33 1092
PfoI T'CCnGG_A
Cuts at: 0 326 359 830 1039 1125
Size: 326 33 471 209 86
Fragments arranged by size:
471 326 209 86 33
PspXI vC'TCGA_Gb
Cuts at: 0 779 1125
Size: 779 346
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 881 1125
Size: 881 244
SfoI GGC'GCC
Cuts at: 0 636 1125
Size: 636 489
SmlI C'TyrA_G
Cuts at: 0 779 1125
Size: 779 346
StyI C'CwwG_G
Cuts at: 0 452 788 903 923 1125
Size: 452 336 115 20 202
Fragments arranged by size:
452 336 202 115 20
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 333 999 1125
Size: 333 666 126
Fragments arranged by size:
666 333 126
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 308 340 694 726 1125
Size: 308 32 354 32 399
Fragments arranged by size:
399 354 308 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 516 1125
Size: 516 609
XhoI C'TCGA_G
Cuts at: 0 779 1125
Size: 779 346
Enzymes that do cut:
AccI AcuI AleI AlfI ApoI AvaI BanI BanII
BbeI BclI BglI Bme1580I BmgBI BmrI BmtI BplI
BpmI Bpu10I BsaI BsaBI BsaHI BsaXI BseRI BsgI
BsiHKAI Bsp1286I BsrDI BsrFI BstBI BstXI BtgI BtsI
EaeI Eco57MI EcoRI FalI FspI HaeII Hin4I KasI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NheI
PfoI PspXI SfiI SfoI SmlI StyI TaqII TstI
XcmI XhoI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AloI AlwNI ApaI ApaLI AscI AseI AsiSI
AvrII BaeI BamHI BbsI BbvCI BcgI BciVI BfrBI
BglII BlpI BpuEI BsaAI BsaWI BseYI BsiEI BsiWI
BsmI BsmBI BspEI BspHI BspMI BsrBI BsrGI BssHII
BssSI BstAPI BstEII BstYI BstZ17I Bsu36I BtgZI ClaI
CspCI DraI DraIII DrdI EagI EarI EciI EcoICRI
EcoNI EcoO109I EcoRV FseI FspAI HincII HindIII HpaI
KpnI MfeI MluI MmeI NdeI NotI NruI NsiI
NspI PacI PasI PciI PflMI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI StuI SwaI TatI Tth111I XbaI XmaI
XmnI ZraI