(Linear) (Six-base) MAPSORT of: KIBB5284.seq  Check: 852  from: 1  to: 567



KIBB5284.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:15  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     223     567 
   Size:        223     344
 
AvaI C'yCGr_G

Cuts at:      0     177     229     567 
   Size:        177      52     338

  Fragments arranged by size: 

                 338     177      52
 
BanII G_rGCy'C

Cuts at:      0      38     335     567 
   Size:         38     297     232

  Fragments arranged by size: 

                 297     232      38
 
BbvCI CC'TCA_GC

Cuts at:      0     302     567 
   Size:        302     265
 
BglI GCCn_nnn'nGGC

Cuts at:      0     255     567 
   Size:        255     312
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     546     567 
   Size:        546      21
 
Bpu10I CC'TnA_GC

Cuts at:      0     302     422     567 
   Size:        302     120     145

  Fragments arranged by size: 

                 302     145     120
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     254     567 
   Size:        254     313
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     319     538     567 
   Size:        319     219      29

  Fragments arranged by size: 

                 319     219      29
 
BsiHKAI G_wGCw'C

Cuts at:      0      38     335     567 
   Size:         38     297     232

  Fragments arranged by size: 

                 297     232      38
 
Bsp1286I G_dGCh'C

Cuts at:      0      38     335     567 
   Size:         38     297     232

  Fragments arranged by size: 

                 297     232      38
 
BstYI r'GATC_y

Cuts at:      0     278     567 
   Size:        278     289
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     151     567 
   Size:        151     416
 
EaeI y'GGCC_r

Cuts at:      0      71     567 
   Size:         71     496
 
EarI CTCTTCn'nnn_

Cuts at:      0     269     548     567 
   Size:        269     279      19

  Fragments arranged by size: 

                 279     269      19
 
EcoICRI GAG'CTC

Cuts at:      0      36     333     567 
   Size:         36     297     234

  Fragments arranged by size: 

                 297     234      36
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     223     546     567 
   Size:        223     323      21

  Fragments arranged by size: 

                 323     223      21
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     362     394     567 
   Size:        362      32     173

  Fragments arranged by size: 

                 362     173      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     167     470     476     567 
   Size:        167     303       6      91

  Fragments arranged by size: 

                 303     167      91       6
 
MslI CAynn'nnrTG

Cuts at:      0     415     567 
   Size:        415     152
 
MspA1I CmG'CkG

Cuts at:      0      42     567 
   Size:         42     525
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     444     567 
   Size:        444     123
 
SacI G_AGCT'C

Cuts at:      0      38     335     567 
   Size:         38     297     232

  Fragments arranged by size: 

                 297     232      38
 
StuI AGG'CCT

Cuts at:      0     559     567 
   Size:        559       8
 
StyI C'CwwG_G

Cuts at:      0     250     567 
   Size:        250     317
 

 Enzymes that do cut:

     AcuI     AvaI    BanII    BbvCI     BglI     BpmI   Bpu10I     BsaI
    BseRI  BsiHKAI Bsp1286I    BstYI     DrdI     EaeI     EarI  EcoICRI
  Eco57MI    Hin4I     MmeI     MslI   MspA1I    PflMI     SacI     StuI
     StyI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BanI
     BbeI     BbsI     BcgI    BciVI     BclI    BfrBI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BmtI     BplI    BpuEI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseYI     BsgI    BsiEI    BsiWI     BsmI
    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I
     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     EagI
     EciI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI    HaeII   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MscI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI     PfoI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    PvuII    RsrII    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI