Restriction map for
pF1KB5292
(Linear) (Six-base) MAPSORT of: KIBB5292.seq Check: 6180 from: 1 to: 1236
KIBB5292.orf
With 182 enzymes: SgfI *
October 7, 2009 14:16 ..
AatII G_ACGT'C
Cuts at: 0 1163 1236
Size: 1163 73
Acc65I G'GTAC_C
Cuts at: 0 403 446 1236
Size: 403 43 790
Fragments arranged by size:
790 403 43
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1039 1236
Size: 1039 197
AhdI GACnn_n'nnGTC
Cuts at: 0 465 1236
Size: 465 771
AleI CACnn'nnGTG
Cuts at: 0 391 1236
Size: 391 845
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 670 702 1236
Size: 670 32 534
Fragments arranged by size:
670 534 32
AlwNI CAG_nnn'CTG
Cuts at: 0 499 666 1236
Size: 499 167 570
Fragments arranged by size:
570 499 167
ApaI G_GGCC'C
Cuts at: 0 197 910 1236
Size: 197 713 326
Fragments arranged by size:
713 326 197
AvaI C'yCGr_G
Cuts at: 0 202 429 1236
Size: 202 227 807
Fragments arranged by size:
807 227 202
BamHI G'GATC_C
Cuts at: 0 68 353 1148 1236
Size: 68 285 795 88
Fragments arranged by size:
795 285 88 68
BanI G'GyrC_C
Cuts at: 0 174 403 446 471 1236
Size: 174 229 43 25 765
Fragments arranged by size:
765 229 174 43 25
BanII G_rGCy'C
Cuts at: 0 124 197 298 910 1236
Size: 124 73 101 612 326
Fragments arranged by size:
612 326 124 101 73
BbsI GAAGACnn'nnnn_
Cuts at: 0 276 633 1093 1155 1236
Size: 276 357 460 62 81
Fragments arranged by size:
460 357 276 81 62
BglI GCCn_nnn'nGGC
Cuts at: 0 946 1103 1236
Size: 946 157 133
Fragments arranged by size:
946 157 133
Bme1580I G_kGCm'C
Cuts at: 0 197 476 910 1236
Size: 197 279 434 326
Fragments arranged by size:
434 326 279 197
BmgBI CAC'GTC
Cuts at: 0 89 1236
Size: 89 1147
BmrI ACTGGGnnnn_n'
Cuts at: 0 225 261 1236
Size: 225 36 975
Fragments arranged by size:
975 225 36
Bpu10I CC'TnA_GC
Cuts at: 0 451 697 1099 1236
Size: 451 246 402 137
Fragments arranged by size:
451 402 246 137
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 196 1051 1236
Size: 196 855 185
Fragments arranged by size:
855 196 185
BsaAI yAC'GTr
Cuts at: 0 393 1236
Size: 393 843
BsaBI GATnn'nnATC
Cuts at: 0 250 1236
Size: 250 986
BsaHI Gr'CG_yC
Cuts at: 0 227 259 1160 1236
Size: 227 32 901 76
Fragments arranged by size:
901 227 76 32
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 280 310 670 700 1236
Size: 280 30 360 30 536
Fragments arranged by size:
536 360 280 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 288 1236
Size: 288 948
BseYI C'CCAG_C
Cuts at: 0 738 1023 1132 1236
Size: 738 285 109 104
Fragments arranged by size:
738 285 109 104
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 29 61 1236
Size: 29 32 1175
Fragments arranged by size:
1175 32 29
BsiEI CG_ry'CG
Cuts at: 0 1173 1236
Size: 1173 63
BsiHKAI G_wGCw'C
Cuts at: 0 212 1236
Size: 212 1024
BsmBI CGTCTCn'nnnn_
Cuts at: 0 159 1236
Size: 159 1077
Bsp1286I G_dGCh'C
Cuts at: 0 124 197 212 298 476 910 1236
Size: 124 73 15 86 178 434 326
Fragments arranged by size:
434 326 178 124 86 73 15
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 519 801 1236
Size: 519 282 435
Fragments arranged by size:
519 435 282
BsrBI CCG'CTC
Cuts at: 0 24 1103 1236
Size: 24 1079 133
Fragments arranged by size:
1079 133 24
BstYI r'GATC_y
Cuts at: 0 68 353 1148 1236
Size: 68 285 795 88
Fragments arranged by size:
795 285 88 68
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 327 555 1236
Size: 327 228 681
Fragments arranged by size:
681 327 228
BtsI GCAGTG_nn'
Cuts at: 0 324 1236
Size: 324 912
EaeI y'GGCC_r
Cuts at: 0 836 1170 1236
Size: 836 334 66
Fragments arranged by size:
836 334 66
EagI C'GGCC_G
Cuts at: 0 1170 1236
Size: 1170 66
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 85 1236
Size: 85 1151
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1039 1236
Size: 1039 197
EcoNI CCTnn'n_nnAGG
Cuts at: 0 454 823 856 1236
Size: 454 369 33 380
Fragments arranged by size:
454 380 369 33
EcoO109I rG'GnC_Cy
Cuts at: 0 132 148 193 312 705 1236
Size: 132 16 45 119 393 531
Fragments arranged by size:
531 393 132 119 45 16
FspI TGC'GCA
Cuts at: 0 716 1236
Size: 716 520
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 280 312 451 483 979 1011 1236
Size: 280 32 139 32 496 32 225
Fragments arranged by size:
496 280 225 139 32 32 32
HincII GTy'rAC
Cuts at: 0 624 1236
Size: 624 612
KpnI G_GTAC'C
Cuts at: 0 407 450 1236
Size: 407 43 786
Fragments arranged by size:
786 407 43
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 89 326 1236
Size: 89 237 910
Fragments arranged by size:
910 237 89
MscI TGG'CCA
Cuts at: 0 838 1236
Size: 838 398
MslI CAynn'nnrTG
Cuts at: 0 391 1114 1236
Size: 391 723 122
Fragments arranged by size:
723 391 122
MspA1I CmG'CkG
Cuts at: 0 75 842 957 1136 1236
Size: 75 767 115 179 100
Fragments arranged by size:
767 179 115 100 75
PasI CC'CwG_GG
Cuts at: 0 504 1236
Size: 504 732
PflMI CCAn_nnn'nTGG
Cuts at: 0 308 1236
Size: 308 928
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 670 702 1236
Size: 670 32 534
Fragments arranged by size:
670 534 32
PpuMI rG'GwC_Cy
Cuts at: 0 132 312 705 1236
Size: 132 180 393 531
Fragments arranged by size:
531 393 180 132
PspOMI G'GGCC_C
Cuts at: 0 193 906 1236
Size: 193 713 330
Fragments arranged by size:
713 330 193
PstI C_TGCA'G
Cuts at: 0 935 1236
Size: 935 301
SanDI GG'GwC_CC
Cuts at: 0 312 705 1236
Size: 312 393 531
Fragments arranged by size:
531 393 312
ScaI AGT'ACT
Cuts at: 0 163 235 1236
Size: 163 72 1001
Fragments arranged by size:
1001 163 72
SexAI A'CCwGG_T
Cuts at: 0 824 1236
Size: 824 412
SfcI C'TryA_G
Cuts at: 0 931 1236
Size: 931 305
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 946 1236
Size: 946 290
SmlI C'TyrA_G
Cuts at: 0 211 1066 1236
Size: 211 855 170
Fragments arranged by size:
855 211 170
StuI AGG'CCT
Cuts at: 0 802 1236
Size: 802 434
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 202 730 1146 1236
Size: 202 528 416 90
Fragments arranged by size:
528 416 202 90
TatI w'GTAC_w
Cuts at: 0 161 233 365 971 1236
Size: 161 72 132 606 265
Fragments arranged by size:
606 265 161 132 72
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1133 1165 1236
Size: 1133 32 71
Fragments arranged by size:
1133 71 32
Tth111I GACn'n_nGTC
Cuts at: 0 105 1236
Size: 105 1131
XmnI GAAnn'nnTTC
Cuts at: 0 682 1236
Size: 682 554
ZraI GAC'GTC
Cuts at: 0 1161 1236
Size: 1161 75
Enzymes that do cut:
AatII Acc65I AcuI AhdI AleI AloI AlwNI ApaI
AvaI BamHI BanI BanII BbsI BglI Bme1580I BmgBI
BmrI Bpu10I BpuEI BsaAI BsaBI BsaHI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrBI
BstYI BtgZI BtsI EaeI EagI EciI Eco57MI EcoNI
EcoO109I FspI Hin4I HincII KpnI MmeI MscI MslI
MspA1I PasI PflMI PpiI PpuMI PspOMI PstI SanDI
ScaI SexAI SfcI SfiI SmlI StuI TaqII TatI
TstI Tth111I XmnI ZraI
Enzymes that do not cut:
AarI AccI AclI AfeI AflII AflIII AgeI AlfI
ApaLI ApoI AscI AseI AsiSI AvrII BaeI BbeI
BbvCI BcgI BciVI BclI BfrBI BglII BlpI BmtI
BplI BpmI BsaI BsaWI BsiWI BsmI BspEI BspHI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstZ17I Bsu36I BtgI ClaI CspCI DraI DraIII
DrdI EarI EcoICRI EcoRI EcoRV FalI FseI FspAI
HaeII HindIII HpaI KasI MfeI MluI NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI
PacI PciI PfoI PmeI PmlI PshAI PsiI PspXI
PsrI PvuI PvuII RsrII SacI SacII SalI SapI
SbfI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI StyI SwaI XbaI XcmI XhoI XmaI