Restriction map for
pF1KB5298
(Linear) (Six-base) MAPSORT of: KIBB5298.seq Check: 3561 from: 1 to: 1902
KIBB5298.orf
With 182 enzymes: SgfI *
February 28, 2007 11:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 666 1360 1902
Size: 666 694 542
Fragments arranged by size:
694 666 542
AccI GT'mk_AC
Cuts at: 0 464 587 1052 1902
Size: 464 123 465 850
Fragments arranged by size:
850 465 464 123
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 207 373 1902
Size: 207 166 1529
Fragments arranged by size:
1529 207 166
AfeI AGC'GCT
Cuts at: 0 1252 1902
Size: 1252 650
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1010 1051 1636 1902
Size: 1010 41 585 266
Fragments arranged by size:
1010 585 266 41
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1 33 1902
Size: 1 32 1869
Fragments arranged by size:
1869 32 1
AlwNI CAG_nnn'CTG
Cuts at: 0 105 768 1152 1902
Size: 105 663 384 750
Fragments arranged by size:
750 663 384 105
ApaI G_GGCC'C
Cuts at: 0 651 1730 1902
Size: 651 1079 172
Fragments arranged by size:
1079 651 172
ApaLI G'TGCA_C
Cuts at: 0 1531 1687 1902
Size: 1531 156 215
Fragments arranged by size:
1531 215 156
AscI GG'CGCG_CC
Cuts at: 0 1446 1902
Size: 1446 456
BanII G_rGCy'C
Cuts at: 0 344 651 710 865 1730 1902
Size: 344 307 59 155 865 172
Fragments arranged by size:
865 344 307 172 155 59
BbvCI CC'TCA_GC
Cuts at: 0 598 752 940 1902
Size: 598 154 188 962
Fragments arranged by size:
962 598 188 154
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 162 196 1902
Size: 162 34 1706
Fragments arranged by size:
1706 162 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1510 1902
Size: 1510 392
BglI GCCn_nnn'nGGC
Cuts at: 0 1003 1443 1679 1902
Size: 1003 440 236 223
Fragments arranged by size:
1003 440 236 223
BglII A'GATC_T
Cuts at: 0 692 1902
Size: 692 1210
BlpI GC'TnA_GC
Cuts at: 0 343 1902
Size: 343 1559
Bme1580I G_kGCm'C
Cuts at: 0 651 1535 1601 1691 1730 1902
Size: 651 884 66 90 39 172
Fragments arranged by size:
884 651 172 90 66 39
BmgBI CAC'GTC
Cuts at: 0 1530 1559 1902
Size: 1530 29 343
Fragments arranged by size:
1530 343 29
BmrI ACTGGGnnnn_n'
Cuts at: 0 982 1881 1902
Size: 982 899 21
Fragments arranged by size:
982 899 21
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 358 634 691 1128 1351 1875 1902
Size: 358 276 57 437 223 524 27
Fragments arranged by size:
524 437 358 276 223 57 27
Bpu10I CC'TnA_GC
Cuts at: 0 349 598 752 940 1902
Size: 349 249 154 188 962
Fragments arranged by size:
962 349 249 188 154
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 510 742 1279 1816 1902
Size: 510 232 537 537 86
Fragments arranged by size:
537 537 510 232 86
BsaI GGTCTCn'nnnn_
Cuts at: 0 622 624 1902
Size: 622 2 1278
Fragments arranged by size:
1278 622 2
BsaAI yAC'GTr
Cuts at: 0 1284 1686 1902
Size: 1284 402 216
Fragments arranged by size:
1284 402 216
BsaBI GATnn'nnATC
Cuts at: 0 1345 1902
Size: 1345 557
BsaWI w'CCGG_w
Cuts at: 0 830 1902
Size: 830 1072
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1 31 865 895 1902
Size: 1 30 834 30 1007
Fragments arranged by size:
1007 834 30 30 1
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 22 586 1462 1891 1902
Size: 22 564 876 429 11
Fragments arranged by size:
876 564 429 22 11
BseYI C'CCAG_C
Cuts at: 0 23 238 787 850 1594 1715 1730 1902
Size: 23 215 549 63 744 121 15 172
Fragments arranged by size:
744 549 215 172 121 63 23 15
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 26 685 700 1539 1902
Size: 26 659 15 839 363
Fragments arranged by size:
839 659 363 26 15
BsiEI CG_ry'CG
Cuts at: 0 883 1359 1902
Size: 883 476 543
Fragments arranged by size:
883 543 476
BsiHKAI G_wGCw'C
Cuts at: 0 344 1040 1166 1535 1691 1772 1902
Size: 344 696 126 369 156 81 130
Fragments arranged by size:
696 369 344 156 130 126 81
BsmI GAATG_Cn'
Cuts at: 0 914 1245 1902
Size: 914 331 657
Fragments arranged by size:
914 657 331
Bsp1286I G_dGCh'C
Cuts at: 0 344 651 710 865 1040 1166 1535 1601
Size: 344 307 59 155 175 126 369 66
Cuts at: 1601 1691 1730 1772 1902
Size: 90 39 42 130
Fragments arranged by size:
369 344 307 175 155 130 126 90
66 59 42 39
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 666 1184 1360 1902
Size: 666 518 176 542
Fragments arranged by size:
666 542 518 176
BsrBI CCG'CTC
Cuts at: 0 1655 1784 1902
Size: 1655 129 118
Fragments arranged by size:
1655 129 118
BsrDI GCAATG_nn'
Cuts at: 0 360 1613 1902
Size: 360 1253 289
Fragments arranged by size:
1253 360 289
BsrFI r'CCGG_y
Cuts at: 0 467 1902
Size: 467 1435
BsrGI T'GTAC_A
Cuts at: 0 357 1902
Size: 357 1545
BssHII G'CGCG_C
Cuts at: 0 1446 1902
Size: 1446 456
BssSI C'ACGA_G
Cuts at: 0 703 1902
Size: 703 1199
BstAPI GCAn_nnn'nTGC
Cuts at: 0 768 1902
Size: 768 1134
BstYI r'GATC_y
Cuts at: 0 392 692 1142 1634 1902
Size: 392 300 450 492 268
Fragments arranged by size:
492 450 392 300 268
BstZ17I GTA'TAC
Cuts at: 0 1053 1902
Size: 1053 849
BtgI C'CryG_G
Cuts at: 0 279 1120 1665 1806 1902
Size: 279 841 545 141 96
Fragments arranged by size:
841 545 279 141 96
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 175 1014 1902
Size: 175 839 888
Fragments arranged by size:
888 839 175
BtsI GCAGTG_nn'
Cuts at: 0 60 992 1389 1902
Size: 60 932 397 513
Fragments arranged by size:
932 513 397 60
DraIII CAC_nnn'GTG
Cuts at: 0 66 285 1539 1902
Size: 66 219 1254 363
Fragments arranged by size:
1254 363 219 66
DrdI GACnn_nn'nnGTC
Cuts at: 0 1075 1902
Size: 1075 827
EaeI y'GGCC_r
Cuts at: 0 809 1356 1650 1902
Size: 809 547 294 252
Fragments arranged by size:
809 547 294 252
EagI C'GGCC_G
Cuts at: 0 1356 1902
Size: 1356 546
EarI CTCTTCn'nnn_
Cuts at: 0 151 260 440 1902
Size: 151 109 180 1462
Fragments arranged by size:
1462 180 151 109
EcoICRI GAG'CTC
Cuts at: 0 342 1902
Size: 342 1560
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 207 358 373 634 691 1128 1351 1875
Size: 207 151 15 261 57 437 223 524
Cuts at: 1875 1902
Size: 27
Fragments arranged by size:
524 437 261 223 207 151 57 27
15
EcoNI CCTnn'n_nnAGG
Cuts at: 0 673 1156 1902
Size: 673 483 746
Fragments arranged by size:
746 673 483
EcoO109I rG'GnC_Cy
Cuts at: 0 270 647 648 756 1726 1727 1811 1902
Size: 270 377 1 108 970 1 84 91
Fragments arranged by size:
970 377 270 108 91 84 1 1
HaeII r_GCGC'y
Cuts at: 0 818 1254 1902
Size: 818 436 648
Fragments arranged by size:
818 648 436
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 679 711 865 897 1831 1863 1902
Size: 679 32 154 32 934 32 39
Fragments arranged by size:
934 679 154 39 32 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 863 1043 1902
Size: 863 180 859
Fragments arranged by size:
863 859 180
MscI TGG'CCA
Cuts at: 0 811 1902
Size: 811 1091
MslI CAynn'nnrTG
Cuts at: 0 1033 1618 1902
Size: 1033 585 284
Fragments arranged by size:
1033 585 284
MspA1I CmG'CkG
Cuts at: 0 137 741 765 1133 1553 1667 1734 1808
Size: 137 604 24 368 420 114 67 74
Cuts at: 1808 1902
Size: 94
Fragments arranged by size:
604 420 368 137 114 94 74 67
24
NotI GC'GGCC_GC
Cuts at: 0 1356 1902
Size: 1356 546
NspI r_CATG'y
Cuts at: 0 1017 1902
Size: 1017 885
PflMI CCAn_nnn'nTGG
Cuts at: 0 1274 1902
Size: 1274 628
PfoI T'CCnGG_A
Cuts at: 0 128 1902
Size: 128 1774
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1 33 1902
Size: 1 32 1869
Fragments arranged by size:
1869 32 1
PpuMI rG'GwC_Cy
Cuts at: 0 270 756 1811 1902
Size: 270 486 1055 91
Fragments arranged by size:
1055 486 270 91
PshAI GACnn'nnGTC
Cuts at: 0 1569 1902
Size: 1569 333
PspOMI G'GGCC_C
Cuts at: 0 647 1726 1902
Size: 647 1079 176
Fragments arranged by size:
1079 647 176
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 61 93 1902
Size: 61 32 1809
Fragments arranged by size:
1809 61 32
PstI C_TGCA'G
Cuts at: 0 764 860 1157 1902
Size: 764 96 297 745
Fragments arranged by size:
764 745 297 96
PvuII CAG'CTG
Cuts at: 0 1553 1734 1902
Size: 1553 181 168
Fragments arranged by size:
1553 181 168
SacI G_AGCT'C
Cuts at: 0 344 1902
Size: 344 1558
SacII CC_GC'GG
Cuts at: 0 1668 1809 1902
Size: 1668 141 93
Fragments arranged by size:
1668 141 93
SanDI GG'GwC_CC
Cuts at: 0 1811 1902
Size: 1811 91
SexAI A'CCwGG_T
Cuts at: 0 1535 1902
Size: 1535 367
SfcI C'TryA_G
Cuts at: 0 760 844 856 1153 1548 1902
Size: 760 84 12 297 395 354
Fragments arranged by size:
760 395 354 297 84 12
SmlI C'TyrA_G
Cuts at: 0 489 721 1294 1795 1902
Size: 489 232 573 501 107
Fragments arranged by size:
573 501 489 232 107
StuI AGG'CCT
Cuts at: 0 1754 1902
Size: 1754 148
StyI C'CwwG_G
Cuts at: 0 1080 1902
Size: 1080 822
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 37 71 426 646 1902
Size: 37 34 355 220 1256
Fragments arranged by size:
1256 355 220 37 34
TatI w'GTAC_w
Cuts at: 0 357 1902
Size: 357 1545
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 271 303 877 909 1902
Size: 271 32 574 32 993
Fragments arranged by size:
993 574 271 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1752 1902
Size: 1752 150
XmnI GAAnn'nnTTC
Cuts at: 0 988 1902
Size: 988 914
Enzymes that do cut:
AarI AccI AcuI AfeI AlfI AloI AlwNI ApaI
ApaLI AscI BanII BbvCI BcgI BciVI BglI BglII
BlpI Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstYI BstZ17I BtgI BtgZI BtsI
DraIII DrdI EaeI EagI EarI EcoICRI Eco57MI EcoNI
EcoO109I HaeII Hin4I MmeI MscI MslI MspA1I NotI
NspI PflMI PfoI PpiI PpuMI PshAI PspOMI PsrI
PstI PvuII SacI SacII SanDI SexAI SfcI SmlI
StuI StyI TaqII TatI TstI XcmI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AflII AflIII AgeI AhdI AleI
ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI
BbeI BbsI BclI BfrBI BmtI BplI BsaHI BsiWI
BsmBI BspEI BspHI BstBI BstEII BstXI Bsu36I ClaI
CspCI DraI EciI EcoRI EcoRV FalI FseI FspI
FspAI HincII HindIII HpaI KasI KpnI MfeI MluI
NaeI NarI NcoI NdeI NgoMIV NheI NruI NsiI
PacI PasI PciI PmeI PmlI PsiI PspXI PvuI
RsrII SalI SapI SbfI ScaI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI
Tth111I XbaI XhoI XmaI ZraI