Restriction map for
pF1KB5299
(Linear) (Six-base) MAPSORT of: KIBB5299.seq Check: 953 from: 1 to: 1356
KIBB5299.orf
With 182 enzymes: SgfI *
February 28, 2007 11:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 167 1356
Size: 167 1189
AccI GT'mk_AC
Cuts at: 0 1014 1356
Size: 1014 342
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 334 382 995 1356
Size: 334 48 613 361
Fragments arranged by size:
613 361 334 48
AfeI AGC'GCT
Cuts at: 0 119 1356
Size: 119 1237
AflIII A'CryG_T
Cuts at: 0 789 1356
Size: 789 567
AleI CACnn'nnGTG
Cuts at: 0 790 1356
Size: 790 566
AlwNI CAG_nnn'CTG
Cuts at: 0 1323 1356
Size: 1323 33
ApaLI G'TGCA_C
Cuts at: 0 505 1356
Size: 505 851
ApoI r'AATT_y
Cuts at: 0 41 1356
Size: 41 1315
AvaI C'yCGr_G
Cuts at: 0 113 586 1356
Size: 113 473 770
Fragments arranged by size:
770 473 113
BamHI G'GATC_C
Cuts at: 0 1113 1356
Size: 1113 243
BanII G_rGCy'C
Cuts at: 0 67 654 1191 1356
Size: 67 587 537 165
Fragments arranged by size:
587 537 165 67
BbsI GAAGACnn'nnnn_
Cuts at: 0 140 935 1356
Size: 140 795 421
Fragments arranged by size:
795 421 140
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 129 163 1356
Size: 129 34 1193
Fragments arranged by size:
1193 129 34
Bme1580I G_kGCm'C
Cuts at: 0 509 1356
Size: 509 847
BmrI ACTGGGnnnn_n'
Cuts at: 0 61 195 1356
Size: 61 134 1161
Fragments arranged by size:
1161 134 61
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 153 392 1356
Size: 153 239 964
Fragments arranged by size:
964 239 153
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 41 201 528 1321 1356
Size: 41 160 327 793 35
Fragments arranged by size:
793 327 160 41 35
Bpu10I CC'TnA_GC
Cuts at: 0 121 653 1356
Size: 121 532 703
Fragments arranged by size:
703 532 121
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 193 1356
Size: 193 1163
BsaI GGTCTCn'nnnn_
Cuts at: 0 963 1356
Size: 963 393
BsaHI Gr'CG_yC
Cuts at: 0 91 1356
Size: 91 1265
BsaWI w'CCGG_w
Cuts at: 0 602 1103 1356
Size: 602 501 253
Fragments arranged by size:
602 501 253
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 308 1356
Size: 308 1048
BseYI C'CCAG_C
Cuts at: 0 735 808 1356
Size: 735 73 548
Fragments arranged by size:
735 548 73
BsiHKAI G_wGCw'C
Cuts at: 0 338 509 654 787 983 1307 1356
Size: 338 171 145 133 196 324 49
Fragments arranged by size:
338 324 196 171 145 133 49
Bsp1286I G_dGCh'C
Cuts at: 0 67 338 509 654 787 983 1191 1307
Size: 67 271 171 145 133 196 208 116
Cuts at: 1307 1356
Size: 49
Fragments arranged by size:
271 208 196 171 145 133 116 67
49
BspEI T'CCGG_A
Cuts at: 0 1103 1356
Size: 1103 253
BspHI T'CATG_A
Cuts at: 0 1277 1356
Size: 1277 79
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 167 1356
Size: 167 1189
BsrBI CCG'CTC
Cuts at: 0 542 1356
Size: 542 814
BsrFI r'CCGG_y
Cuts at: 0 569 1055 1356
Size: 569 486 301
Fragments arranged by size:
569 486 301
BstAPI GCAn_nnn'nTGC
Cuts at: 0 987 1356
Size: 987 369
BstEII G'GTnAC_C
Cuts at: 0 548 1356
Size: 548 808
BstXI CCAn_nnnn'nTGG
Cuts at: 0 179 1125 1356
Size: 179 946 231
Fragments arranged by size:
946 231 179
BstYI r'GATC_y
Cuts at: 0 1113 1356
Size: 1113 243
BtgI C'CryG_G
Cuts at: 0 494 716 851 1356
Size: 494 222 135 505
Fragments arranged by size:
505 494 222 135
BtsI GCAGTG_nn'
Cuts at: 0 729 1356
Size: 729 627
ClaI AT'CG_AT
Cuts at: 0 312 1356
Size: 312 1044
DraIII CAC_nnn'GTG
Cuts at: 0 852 1356
Size: 852 504
EaeI y'GGCC_r
Cuts at: 0 1284 1356
Size: 1284 72
EarI CTCTTCn'nnn_
Cuts at: 0 971 1356
Size: 971 385
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 133 616 1356
Size: 133 483 740
Fragments arranged by size:
740 483 133
EcoICRI GAG'CTC
Cuts at: 0 652 1356
Size: 652 704
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 41 201 334 382 528 995 1321 1356
Size: 41 160 133 48 146 467 326 35
Fragments arranged by size:
467 326 160 146 133 48 41 35
EcoNI CCTnn'n_nnAGG
Cuts at: 0 323 1356
Size: 323 1033
EcoO109I rG'GnC_Cy
Cuts at: 0 479 658 1356
Size: 479 179 698
Fragments arranged by size:
698 479 179
FspI TGC'GCA
Cuts at: 0 155 1356
Size: 155 1201
HaeII r_GCGC'y
Cuts at: 0 121 1356
Size: 121 1235
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 32 1075 1098 1107 1130 1165 1197 1356
Size: 32 1043 23 9 23 35 32 159
Fragments arranged by size:
1043 159 35 32 32 23 23 9
MfeI C'AATT_G
Cuts at: 0 242 1356
Size: 242 1114
MluI A'CGCG_T
Cuts at: 0 789 1356
Size: 789 567
MslI CAynn'nnrTG
Cuts at: 0 326 790 1356
Size: 326 464 566
Fragments arranged by size:
566 464 326
MspA1I CmG'CkG
Cuts at: 0 594 801 1356
Size: 594 207 555
Fragments arranged by size:
594 555 207
NcoI C'CATG_G
Cuts at: 0 716 1356
Size: 716 640
PflMI CCAn_nnn'nTGG
Cuts at: 0 954 1323 1356
Size: 954 369 33
Fragments arranged by size:
954 369 33
PpuMI rG'GwC_Cy
Cuts at: 0 479 658 1356
Size: 479 179 698
Fragments arranged by size:
698 479 179
PspXI vC'TCGA_Gb
Cuts at: 0 113 1356
Size: 113 1243
PvuII CAG'CTG
Cuts at: 0 801 1356
Size: 801 555
SacI G_AGCT'C
Cuts at: 0 654 1356
Size: 654 702
SanDI GG'GwC_CC
Cuts at: 0 658 1356
Size: 658 698
SapI GCTCTTCn'nnn_
Cuts at: 0 971 1356
Size: 971 385
SfcI C'TryA_G
Cuts at: 0 1011 1356
Size: 1011 345
SmlI C'TyrA_G
Cuts at: 0 113 208 1356
Size: 113 95 1148
Fragments arranged by size:
1148 113 95
SspI AAT'ATT
Cuts at: 0 1100 1356
Size: 1100 256
StyI C'CwwG_G
Cuts at: 0 716 1356
Size: 716 640
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 383 415 676 708 779 811 1297 1329
Size: 383 32 261 32 71 32 486 32
Cuts at: 1329 1356
Size: 27
Fragments arranged by size:
486 383 261 71 32 32 32 32
27
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 492 1356
Size: 492 864
XhoI C'TCGA_G
Cuts at: 0 113 1356
Size: 113 1243
XmnI GAAnn'nnTTC
Cuts at: 0 347 1356
Size: 347 1009
Enzymes that do cut:
AarI AccI AcuI AfeI AflIII AleI AlwNI ApaLI
ApoI AvaI BamHI BanII BbsI BcgI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaHI BsaWI BseRI
BseYI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrFI
BstAPI BstEII BstXI BstYI BtgI BtsI ClaI DraIII
EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FspI
HaeII Hin4I MfeI MluI MslI MspA1I NcoI PflMI
PpuMI PspXI PvuII SacI SanDI SapI SfcI SmlI
SspI StyI TstI XcmI XhoI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AflII AgeI AhdI AlfI AloI
ApaI AscI AseI AsiSI AvrII BaeI BanI BbeI
BbvCI BciVI BclI BfrBI BglI BglII BlpI BmgBI
BmtI BsaAI BsaBI BsaXI BsgI BsiEI BsiWI BsmI
BsmBI BsrDI BsrGI BssHII BssSI BstBI BstZ17I Bsu36I
BtgZI CspCI DraI DrdI EagI EcoRI EcoRV FalI
FseI FspAI HincII HindIII HpaI KasI KpnI MmeI
MscI NaeI NarI NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PasI PciI PfoI PmeI PmlI
PpiI PshAI PsiI PspOMI PsrI PstI PvuI RsrII
SacII SalI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI StuI SwaI
TaqII TatI Tth111I XbaI XmaI ZraI