Restriction map for
pF1KB5307
(Linear) (Six-base) MAPSORT of: KIBB5307.seq Check: 1055 from: 1 to: 1236
KIBB5307.orf
With 182 enzymes: SgfI *
October 7, 2009 14:16 ..
AatII G_ACGT'C
Cuts at: 0 97 1187 1236
Size: 97 1090 49
Fragments arranged by size:
1090 97 49
AccI GT'mk_AC
Cuts at: 0 1187 1236
Size: 1187 49
Acc65I G'GTAC_C
Cuts at: 0 275 1236
Size: 275 961
AfeI AGC'GCT
Cuts at: 0 136 1236
Size: 136 1100
AlwNI CAG_nnn'CTG
Cuts at: 0 405 474 1164 1236
Size: 405 69 690 72
Fragments arranged by size:
690 405 72 69
ApaI G_GGCC'C
Cuts at: 0 87 1236
Size: 87 1149
AvaI C'yCGr_G
Cuts at: 0 31 128 1236
Size: 31 97 1108
Fragments arranged by size:
1108 97 31
BanI G'GyrC_C
Cuts at: 0 275 1069 1236
Size: 275 794 167
Fragments arranged by size:
794 275 167
BanII G_rGCy'C
Cuts at: 0 87 1236
Size: 87 1149
BbeI G_GCGC'C
Cuts at: 0 1073 1236
Size: 1073 163
BbvCI CC'TCA_GC
Cuts at: 0 19 1007 1236
Size: 19 988 229
Fragments arranged by size:
988 229 19
BclI T'GATC_A
Cuts at: 0 926 1236
Size: 926 310
BfrBI ATG'CAT
Cuts at: 0 1062 1236
Size: 1062 174
BglII A'GATC_T
Cuts at: 0 969 1236
Size: 969 267
Bme1580I G_kGCm'C
Cuts at: 0 87 1236
Size: 87 1149
BmgBI CAC'GTC
Cuts at: 0 1115 1236
Size: 1115 121
BmrI ACTGGGnnnn_n'
Cuts at: 0 441 1068 1236
Size: 441 627 168
Fragments arranged by size:
627 441 168
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 213 323 1236
Size: 213 110 913
Fragments arranged by size:
913 213 110
Bpu10I CC'TnA_GC
Cuts at: 0 19 1007 1236
Size: 19 988 229
Fragments arranged by size:
988 229 19
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 814 1236
Size: 814 422
BsaI GGTCTCn'nnnn_
Cuts at: 0 1151 1236
Size: 1151 85
BsaAI yAC'GTr
Cuts at: 0 117 372 520 1236
Size: 117 255 148 716
Fragments arranged by size:
716 255 148 117
BsaBI GATnn'nnATC
Cuts at: 0 920 1236
Size: 920 316
BsaHI Gr'CG_yC
Cuts at: 0 10 94 717 1070 1184 1236
Size: 10 84 623 353 114 52
Fragments arranged by size:
623 353 114 84 52 10
BsaWI w'CCGG_w
Cuts at: 0 1229 1236
Size: 1229 7
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 62 92 1236
Size: 62 30 1144
Fragments arranged by size:
1144 62 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 36 1236
Size: 36 1200
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 352 1236
Size: 352 884
BsiHKAI G_wGCw'C
Cuts at: 0 273 731 1236
Size: 273 458 505
Fragments arranged by size:
505 458 273
BsmI GAATG_Cn'
Cuts at: 0 840 1236
Size: 840 396
BsmBI CGTCTCn'nnnn_
Cuts at: 0 676 1236
Size: 676 560
Bsp1286I G_dGCh'C
Cuts at: 0 87 273 731 1236
Size: 87 186 458 505
Fragments arranged by size:
505 458 186 87
BspEI T'CCGG_A
Cuts at: 0 1229 1236
Size: 1229 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 287 1199 1236
Size: 287 912 37
Fragments arranged by size:
912 287 37
BsrBI CCG'CTC
Cuts at: 0 26 48 89 239 1236
Size: 26 22 41 150 997
Fragments arranged by size:
997 150 41 26 22
BsrDI GCAATG_nn'
Cuts at: 0 747 1236
Size: 747 489
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1164 1236
Size: 1164 72
BstEII G'GTnAC_C
Cuts at: 0 495 1236
Size: 495 741
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1210 1236
Size: 1210 26
BstYI r'GATC_y
Cuts at: 0 935 969 1236
Size: 935 34 267
Fragments arranged by size:
935 267 34
BtgI C'CryG_G
Cuts at: 0 945 1236
Size: 945 291
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 888 1236
Size: 888 348
ClaI AT'CG_AT
Cuts at: 0 1208 1236
Size: 1208 28
DraIII CAC_nnn'GTG
Cuts at: 0 117 1236
Size: 117 1119
EaeI y'GGCC_r
Cuts at: 0 98 170 234 608 765 1047 1212 1236
Size: 98 72 64 374 157 282 165 24
Fragments arranged by size:
374 282 165 157 98 72 64 24
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 193 451 1189 1236
Size: 193 258 738 47
Fragments arranged by size:
738 258 193 47
EcoO109I rG'GnC_Cy
Cuts at: 0 64 513 1023 1081 1236
Size: 64 449 510 58 155
Fragments arranged by size:
510 449 155 64 58
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 663 1236
Size: 663 573
HaeII r_GCGC'y
Cuts at: 0 138 628 1073 1236
Size: 138 490 445 163
Fragments arranged by size:
490 445 163 138
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 12 44 56 88 1236
Size: 12 32 12 32 1148
Fragments arranged by size:
1148 32 32 12 12
HindIII A'AGCT_T
Cuts at: 0 1224 1236
Size: 1224 12
KasI G'GCGC_C
Cuts at: 0 1069 1236
Size: 1069 167
KpnI G_GTAC'C
Cuts at: 0 279 1236
Size: 279 957
MscI TGG'CCA
Cuts at: 0 610 767 1049 1236
Size: 610 157 282 187
Fragments arranged by size:
610 282 187 157
MslI CAynn'nnrTG
Cuts at: 0 220 374 488 734 796 1208 1236
Size: 220 154 114 246 62 412 28
Fragments arranged by size:
412 246 220 154 114 62 28
MspA1I CmG'CkG
Cuts at: 0 187 329 459 471 1155 1236
Size: 187 142 130 12 684 81
Fragments arranged by size:
684 187 142 130 81 12
NarI GG'CG_CC
Cuts at: 0 1070 1236
Size: 1070 166
NsiI A_TGCA'T
Cuts at: 0 1064 1236
Size: 1064 172
NspI r_CATG'y
Cuts at: 0 606 1062 1236
Size: 606 456 174
Fragments arranged by size:
606 456 174
PmlI CAC'GTG
Cuts at: 0 117 372 1236
Size: 117 255 864
Fragments arranged by size:
864 255 117
PpuMI rG'GwC_Cy
Cuts at: 0 64 1023 1081 1236
Size: 64 959 58 155
Fragments arranged by size:
959 155 64 58
PspOMI G'GGCC_C
Cuts at: 0 83 1236
Size: 83 1153
PstI C_TGCA'G
Cuts at: 0 284 1154 1160 1169 1236
Size: 284 870 6 9 67
Fragments arranged by size:
870 284 67 9 6
PvuII CAG'CTG
Cuts at: 0 459 471 1155 1236
Size: 459 12 684 81
Fragments arranged by size:
684 459 81 12
SanDI GG'GwC_CC
Cuts at: 0 64 1236
Size: 64 1172
SbfI CC_TGCA'GG
Cuts at: 0 1169 1236
Size: 1169 67
ScaI AGT'ACT
Cuts at: 0 451 1236
Size: 451 785
SfcI C'TryA_G
Cuts at: 0 280 1150 1156 1165 1236
Size: 280 870 6 9 71
Fragments arranged by size:
870 280 71 9 6
SfoI GGC'GCC
Cuts at: 0 1071 1236
Size: 1071 165
SmaI CCC'GGG
Cuts at: 0 33 1236
Size: 33 1203
SmlI C'TyrA_G
Cuts at: 0 829 1236
Size: 829 407
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 690 1236
Size: 690 546
TatI w'GTAC_w
Cuts at: 0 449 664 1236
Size: 449 215 572
Fragments arranged by size:
572 449 215
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 62 94 1236
Size: 62 32 1142
Fragments arranged by size:
1142 62 32
Tth111I GACn'n_nGTC
Cuts at: 0 649 979 1236
Size: 649 330 257
Fragments arranged by size:
649 330 257
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 618 1236
Size: 618 618
XmaI C'CCGG_G
Cuts at: 0 31 1236
Size: 31 1205
ZraI GAC'GTC
Cuts at: 0 95 1185 1236
Size: 95 1090 51
Fragments arranged by size:
1090 95 51
Enzymes that do cut:
AatII AccI Acc65I AfeI AlwNI ApaI AvaI BanI
BanII BbeI BbvCI BclI BfrBI BglII Bme1580I BmgBI
BmrI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BsgI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspMI BsrBI BsrDI BstAPI BstEII BstXI BstYI
BtgI BtgZI ClaI DraIII EaeI EciI EcoO109I FalI
HaeII Hin4I HindIII KasI KpnI MscI MslI MspA1I
NarI NsiI NspI PmlI PpuMI PspOMI PstI PvuII
SanDI SbfI ScaI SfcI SfoI SmaI SmlI TaqII
TatI TstI Tth111I XcmI XmaI ZraI
Enzymes that do not cut:
AarI AclI AcuI AflII AflIII AgeI AhdI AleI
AlfI AloI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbsI BcgI BciVI BglI BlpI BmtI
BpmI BseYI BsiEI BsiWI BspHI BsrFI BsrGI BssHII
BssSI BstBI BstZ17I Bsu36I BtsI CspCI DraI DrdI
EagI EarI EcoICRI Eco57MI EcoNI EcoRI EcoRV FseI
FspI FspAI HincII HpaI MfeI MluI MmeI NaeI
NcoI NdeI NgoMIV NheI NotI NruI PacI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PspXI
PsrI PvuI RsrII SacI SacII SalI SapI SexAI
SfiI SgfI SgrAI SnaBI SpeI SphI SrfI SspI
StuI StyI SwaI XbaI XhoI XmnI