(Linear) (Six-base) MAPSORT of: KIBB5316.seq  Check: 9886  from: 1  to: 1350



KIBB5316.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:45  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      83    1350 
   Size:         83    1267
 
ApaLI G'TGCA_C

Cuts at:      0    1274    1350 
   Size:       1274      76
 
ApoI r'AATT_y

Cuts at:      0     372     409     530     623     769     941     957     994 
   Size:        372      37     121      93     146     172      16      37

Cuts at:    994    1314    1350 
   Size:        320      36

  Fragments arranged by size: 

                 372     320     172     146     121      93      37      37
                  36      16
 
AseI AT'TA_AT

Cuts at:      0     335    1105    1350 
   Size:        335     770     245

  Fragments arranged by size: 

                 770     335     245
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     827     861    1350 
   Size:        827      34     489

  Fragments arranged by size: 

                 827     489      34
 
BclI T'GATC_A

Cuts at:      0    1227    1350 
   Size:       1227     123
 
Bme1580I G_kGCm'C

Cuts at:      0    1278    1350 
   Size:       1278      72
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     777    1350 
   Size:        777     573
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      52    1350 
   Size:         52    1298
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     323    1210    1271    1350 
   Size:        323     887      61      79

  Fragments arranged by size: 

                 887     323      79      61
 
BsiHKAI G_wGCw'C

Cuts at:      0    1278    1350 
   Size:       1278      72
 
Bsp1286I G_dGCh'C

Cuts at:      0    1278    1350 
   Size:       1278      72
 
BsrDI GCAATG_nn'

Cuts at:      0     956    1350 
   Size:        956     394
 
BsrGI T'GTAC_A

Cuts at:      0     632    1350 
   Size:        632     718
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0      44    1350 
   Size:         44    1306
 
BtsI GCAGTG_nn'

Cuts at:      0    1198    1350 
   Size:       1198     152
 
DraI TTT'AAA

Cuts at:      0     207    1350 
   Size:        207    1143
 
EaeI y'GGCC_r

Cuts at:      0    1218    1350 
   Size:       1218     132
 
EarI CTCTTCn'nnn_

Cuts at:      0    1058    1258    1350 
   Size:       1058     200      92

  Fragments arranged by size: 

                1058     200      92
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      83    1350 
   Size:         83    1267
 
EcoRI G'AATT_C

Cuts at:      0     372     409    1350 
   Size:        372      37     941

  Fragments arranged by size: 

                 941     372      37
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     252    1350 
   Size:        252    1098
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      58      90     340     372    1350 
   Size:         58      32     250      32     978

  Fragments arranged by size: 

                 978     250      58      32      32
 
HincII GTy'rAC

Cuts at:      0     245    1236    1350 
   Size:        245     991     114

  Fragments arranged by size: 

                 991     245     114
 
HindIII A'AGCT_T

Cuts at:      0     431     481     520    1350 
   Size:        431      50      39     830

  Fragments arranged by size: 

                 830     431      50      39
 
PacI TTA_AT'TAA

Cuts at:      0    1109    1350 
   Size:       1109     241
 
PsiI TTA'TAA

Cuts at:      0    1099    1350 
   Size:       1099     251
 
PstI C_TGCA'G

Cuts at:      0     407    1350 
   Size:        407     943
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1058    1350 
   Size:       1058     292
 
ScaI AGT'ACT

Cuts at:      0     106    1350 
   Size:        106    1244
 
SfcI C'TryA_G

Cuts at:      0     403    1331    1350 
   Size:        403     928      19

  Fragments arranged by size: 

                 928     403      19
 
SmlI C'TyrA_G

Cuts at:      0     756    1350 
   Size:        756     594
 
SspI AAT'ATT

Cuts at:      0     856    1350 
   Size:        856     494
 
TatI w'GTAC_w

Cuts at:      0     104     632    1350 
   Size:        104     528     718

  Fragments arranged by size: 

                 718     528     104
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     565    1350 
   Size:        565     785
 
XmnI GAAnn'nnTTC

Cuts at:      0     318    1004    1350 
   Size:        318     686     346

  Fragments arranged by size: 

                 686     346     318
 

 Enzymes that do cut:

     AcuI    ApaLI     ApoI     AseI     BcgI     BclI Bme1580I    BpuEI
    BseRI     BsgI  BsiHKAI Bsp1286I    BsrDI    BsrGI    BtgZI     BtsI
     DraI     EaeI     EarI  Eco57MI    EcoRI     FalI    Hin4I   HincII
  HindIII     PacI     PsiI     PstI     SapI     ScaI     SfcI     SmlI
     SspI     TatI     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI     AscI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI
     BbsI    BbvCI    BciVI    BfrBI     BglI    BglII     BlpI    BmgBI
     BmrI     BmtI     BplI     BpmI   Bpu10I     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseYI    BsiEI    BsiWI     BsmI    BsmBI
    BspEI    BspHI    BspMI    BsrBI    BsrFI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI     ClaI
    CspCI   DraIII     DrdI     EagI     EciI  EcoICRI    EcoNI EcoO109I
    EcoRV     FseI     FspI    FspAI    HaeII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI
     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI
    PshAI   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SbfI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     StuI     StyI
     SwaI    TaqII     TstI  Tth111I     XbaI     XhoI     XmaI     ZraI