(Linear) (Six-base) MAPSORT of: KIBB5318.seq  Check: 1780  from: 1  to: 1275



KIBB5318.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:57  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     713    1275 
   Size:        713     562
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     100     170     185    1275 
   Size:        100      70      15    1090

  Fragments arranged by size: 

                1090     100      70      15
 
AhdI GACnn_n'nnGTC

Cuts at:      0     768    1275 
   Size:        768     507
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1177    1275 
   Size:       1177      98
 
ApoI r'AATT_y

Cuts at:      0     307     552     558     853     953    1275 
   Size:        307     245       6     295     100     322

  Fragments arranged by size: 

                 322     307     295     245     100       6
 
AvaI C'yCGr_G

Cuts at:      0     242    1275 
   Size:        242    1033
 
BanI G'GyrC_C

Cuts at:      0     701    1275 
   Size:        701     574
 
BclI T'GATC_A

Cuts at:      0     741    1275 
   Size:        741     534
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     119    1275 
   Size:        119    1156
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1070    1275 
   Size:       1070     205
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     547     977    1275 
   Size:        547     430     298

  Fragments arranged by size: 

                 547     430     298
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      49    1275 
   Size:         49    1226
 
BseYI C'CCAG_C

Cuts at:      0     198    1275 
   Size:        198    1077
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     694    1275 
   Size:        694     581
 
BsiHKAI G_wGCw'C

Cuts at:      0     387    1275 
   Size:        387     888
 
Bsp1286I G_dGCh'C

Cuts at:      0     387    1275 
   Size:        387     888
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     713    1275 
   Size:        713     562
 
BsrDI GCAATG_nn'

Cuts at:      0     413    1275 
   Size:        413     862
 
BsrFI r'CCGG_y

Cuts at:      0     868    1275 
   Size:        868     407
 
BssSI C'ACGA_G

Cuts at:      0     712    1275 
   Size:        712     563
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     708    1069    1275 
   Size:        708     361     206

  Fragments arranged by size: 

                 708     361     206
 
BstBI TT'CG_AA

Cuts at:      0     935    1275 
   Size:        935     340
 
BstEII G'GTnAC_C

Cuts at:      0     520    1275 
   Size:        520     755
 
BstYI r'GATC_y

Cuts at:      0     727     900    1270    1275 
   Size:        727     173     370       5

  Fragments arranged by size: 

                 727     370     173       5
 
BtsI GCAGTG_nn'

Cuts at:      0     812    1115    1275 
   Size:        812     303     160

  Fragments arranged by size: 

                 812     303     160
 
DraIII CAC_nnn'GTG

Cuts at:      0     801    1275 
   Size:        801     474
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     210    1275 
   Size:        210    1065
 
EaeI y'GGCC_r

Cuts at:      0     669    1275 
   Size:        669     606
 
EarI CTCTTCn'nnn_

Cuts at:      0     119    1226    1275 
   Size:        119    1107      49

  Fragments arranged by size: 

                1107     119      49
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     100     170     185    1070    1275 
   Size:        100      70      15     885     205

  Fragments arranged by size: 

                 885     205     100      70      15
 
EcoRI G'AATT_C

Cuts at:      0     552    1275 
   Size:        552     723
 
FspI TGC'GCA

Cuts at:      0     809    1275 
   Size:        809     466
 
FspAI rTGC'GCAy

Cuts at:      0     809    1275 
   Size:        809     466
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      96     128     555     587     754     786    1219    1251 
   Size:         96      32     427      32     167      32     433      32

Cuts at:   1251    1275 
   Size:         24

  Fragments arranged by size: 

                 433     427     167      96      32      32      32      32
                  24
 
HincII GTy'rAC

Cuts at:      0     825    1275 
   Size:        825     450
 
MscI TGG'CCA

Cuts at:      0     671    1275 
   Size:        671     604
 
MslI CAynn'nnrTG

Cuts at:      0     947    1275 
   Size:        947     328
 
MspA1I CmG'CkG

Cuts at:      0     679    1275 
   Size:        679     596
 
NspI r_CATG'y

Cuts at:      0     396    1275 
   Size:        396     879
 
PfoI T'CCnGG_A

Cuts at:      0     547    1275 
   Size:        547     728
 
PspXI vC'TCGA_Gb

Cuts at:      0     242    1275 
   Size:        242    1033
 
PvuII CAG'CTG

Cuts at:      0     679    1275 
   Size:        679     596
 
SapI GCTCTTCn'nnn_

Cuts at:      0     119    1275 
   Size:        119    1156
 
SexAI A'CCwGG_T

Cuts at:      0      95     766    1275 
   Size:         95     671     509

  Fragments arranged by size: 

                 671     509      95
 
SfcI C'TryA_G

Cuts at:      0     846    1275 
   Size:        846     429
 
SmlI C'TyrA_G

Cuts at:      0     242    1275 
   Size:        242    1033
 
SspI AAT'ATT

Cuts at:      0    1218    1275 
   Size:       1218      57
 
StyI C'CwwG_G

Cuts at:      0    1156    1275 
   Size:       1156     119
 
TatI w'GTAC_w

Cuts at:      0     246    1275 
   Size:        246    1029
 
XhoI C'TCGA_G

Cuts at:      0     242    1275 
   Size:        242    1033
 
XmnI GAAnn'nnTTC

Cuts at:      0     857     989    1275 
   Size:        857     132     286

  Fragments arranged by size: 

                 857     286     132
 

 Enzymes that do cut:

     AarI     AcuI     AhdI    AlwNI     ApoI     AvaI     BanI     BclI
     BmrI     BpmI     BsaI    BseRI    BseYI     BsgI  BsiHKAI Bsp1286I
    BspMI    BsrDI    BsrFI    BssSI   BstAPI    BstBI   BstEII    BstYI
     BtsI   DraIII     DrdI     EaeI     EarI  Eco57MI    EcoRI     FspI
    FspAI    Hin4I   HincII     MscI     MslI   MspA1I     NspI     PfoI
    PspXI    PvuII     SapI    SexAI     SfcI     SmlI     SspI     StyI
     TatI     XhoI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AleI     AlfI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI
    AvrII     BaeI    BamHI    BanII     BbeI     BbsI    BbvCI     BcgI
    BciVI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI     BmtI
     BplI   Bpu10I    BpuEI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrBI    BsrGI
   BssHII    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI    CspCI
     DraI     EagI     EciI  EcoICRI    EcoNI EcoO109I    EcoRV     FalI
     FseI    HaeII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     PacI     PasI     PciI    PflMI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI     PsrI     PstI     PvuI
    RsrII     SacI    SacII     SalI    SanDI     SbfI     ScaI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI
     StuI     SwaI    TaqII     TstI  Tth111I     XbaI     XcmI     XmaI
     ZraI