Restriction map for
pF1KB5324
(Linear) (Six-base) MAPSORT of: KIBB5324.seq Check: 9773 from: 1 to: 1380
KIBB5324.orf
With 182 enzymes: SgfI *
February 28, 2007 11:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 27 1380
Size: 27 1353
Acc65I G'GTAC_C
Cuts at: 0 223 1380
Size: 223 1157
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 658 1380
Size: 658 722
AlwNI CAG_nnn'CTG
Cuts at: 0 498 742 1380
Size: 498 244 638
Fragments arranged by size:
638 498 244
ApaI G_GGCC'C
Cuts at: 0 474 795 1380
Size: 474 321 585
Fragments arranged by size:
585 474 321
ApaLI G'TGCA_C
Cuts at: 0 612 1354 1380
Size: 612 742 26
Fragments arranged by size:
742 612 26
AvaI C'yCGr_G
Cuts at: 0 925 1186 1380
Size: 925 261 194
Fragments arranged by size:
925 261 194
BanI G'GyrC_C
Cuts at: 0 48 223 1380
Size: 48 175 1157
Fragments arranged by size:
1157 175 48
BanII G_rGCy'C
Cuts at: 0 167 276 474 682 751 795 964 1380
Size: 167 109 198 208 69 44 169 416
Fragments arranged by size:
416 208 198 169 167 109 69 44
BbsI GAAGACnn'nnnn_
Cuts at: 0 1063 1380
Size: 1063 317
BbvCI CC'TCA_GC
Cuts at: 0 743 1380
Size: 743 637
BclI T'GATC_A
Cuts at: 0 902 1380
Size: 902 478
BlpI GC'TnA_GC
Cuts at: 0 193 1380
Size: 193 1187
Bme1580I G_kGCm'C
Cuts at: 0 283 474 616 795 1358 1380
Size: 283 191 142 179 563 22
Fragments arranged by size:
563 283 191 179 142 22
BmrI ACTGGGnnnn_n'
Cuts at: 0 279 1380
Size: 279 1101
BmtI G_CTAG'C
Cuts at: 0 112 523 1380
Size: 112 411 857
Fragments arranged by size:
857 411 112
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 906 1380
Size: 906 474
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 749 1380
Size: 749 631
Bpu10I CC'TnA_GC
Cuts at: 0 391 743 1380
Size: 391 352 637
Fragments arranged by size:
637 391 352
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 151 1380
Size: 151 1229
BsaI GGTCTCn'nnnn_
Cuts at: 0 734 1183 1380
Size: 734 449 197
Fragments arranged by size:
734 449 197
BsaAI yAC'GTr
Cuts at: 0 251 1380
Size: 251 1129
BsaHI Gr'CG_yC
Cuts at: 0 688 1380
Size: 688 692
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 832 862 1380
Size: 832 30 518
Fragments arranged by size:
832 518 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 752 895 1380
Size: 752 143 485
Fragments arranged by size:
752 485 143
BseYI C'CCAG_C
Cuts at: 0 474 523 551 1203 1307 1380
Size: 474 49 28 652 104 73
Fragments arranged by size:
652 474 104 73 49 28
BsiHKAI G_wGCw'C
Cuts at: 0 167 467 616 657 964 1358 1380
Size: 167 300 149 41 307 394 22
Fragments arranged by size:
394 307 300 167 149 41 22
BsmBI CGTCTCn'nnnn_
Cuts at: 0 679 1311 1380
Size: 679 632 69
Fragments arranged by size:
679 632 69
Bsp1286I G_dGCh'C
Cuts at: 0 167 276 283 467 474 616 657 682
Size: 167 109 7 184 7 142 41 25
Cuts at: 682 751 795 964 1358 1380
Size: 69 44 169 394 22
Fragments arranged by size:
394 184 169 167 142 109 69 44
41 25 22 7 7
BspHI T'CATG_A
Cuts at: 0 338 1380
Size: 338 1042
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 27 137 263 1380
Size: 27 110 126 1117
Fragments arranged by size:
1117 126 110 27
BsrBI CCG'CTC
Cuts at: 0 510 1380
Size: 510 870
BsrDI GCAATG_nn'
Cuts at: 0 333 1380
Size: 333 1047
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1113 1380
Size: 1113 267
BstYI r'GATC_y
Cuts at: 0 1331 1380
Size: 1331 49
BtgI C'CryG_G
Cuts at: 0 92 578 851 955 1029 1380
Size: 92 486 273 104 74 351
Fragments arranged by size:
486 351 273 104 92 74
BtsI GCAGTG_nn'
Cuts at: 0 535 1380
Size: 535 845
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 684 719 1380
Size: 684 35 661
Fragments arranged by size:
684 661 35
EaeI y'GGCC_r
Cuts at: 0 320 1046 1380
Size: 320 726 334
Fragments arranged by size:
726 334 320
EarI CTCTTCn'nnn_
Cuts at: 0 490 824 830 1380
Size: 490 334 6 550
Fragments arranged by size:
550 490 334 6
EcoICRI GAG'CTC
Cuts at: 0 165 962 1380
Size: 165 797 418
Fragments arranged by size:
797 418 165
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 658 749 1380
Size: 658 91 631
Fragments arranged by size:
658 631 91
EcoO109I rG'GnC_Cy
Cuts at: 0 61 471 792 936 1380
Size: 61 410 321 144 444
Fragments arranged by size:
444 410 321 144 61
HaeII r_GCGC'y
Cuts at: 0 1344 1380
Size: 1344 36
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 832 864 1380
Size: 832 32 516
Fragments arranged by size:
832 516 32
HincII GTy'rAC
Cuts at: 0 156 1227 1380
Size: 156 1071 153
Fragments arranged by size:
1071 156 153
KpnI G_GTAC'C
Cuts at: 0 227 1380
Size: 227 1153
MscI TGG'CCA
Cuts at: 0 322 1048 1380
Size: 322 726 332
Fragments arranged by size:
726 332 322
MslI CAynn'nnrTG
Cuts at: 0 577 607 1150 1380
Size: 577 30 543 230
Fragments arranged by size:
577 543 230 30
MspA1I CmG'CkG
Cuts at: 0 773 818 1044 1307 1380
Size: 773 45 226 263 73
Fragments arranged by size:
773 263 226 73 45
NcoI C'CATG_G
Cuts at: 0 578 851 1380
Size: 578 273 529
Fragments arranged by size:
578 529 273
NheI G'CTAG_C
Cuts at: 0 108 519 1380
Size: 108 411 861
Fragments arranged by size:
861 411 108
PfoI T'CCnGG_A
Cuts at: 0 1274 1380
Size: 1274 106
PpuMI rG'GwC_Cy
Cuts at: 0 61 1380
Size: 61 1319
PspOMI G'GGCC_C
Cuts at: 0 470 791 1380
Size: 470 321 589
Fragments arranged by size:
589 470 321
PstI C_TGCA'G
Cuts at: 0 809 1380
Size: 809 571
PvuII CAG'CTG
Cuts at: 0 818 1044 1380
Size: 818 226 336
Fragments arranged by size:
818 336 226
SacI G_AGCT'C
Cuts at: 0 167 964 1380
Size: 167 797 416
Fragments arranged by size:
797 416 167
SanDI GG'GwC_CC
Cuts at: 0 61 1380
Size: 61 1319
SexAI A'CCwGG_T
Cuts at: 0 497 1380
Size: 497 883
SfcI C'TryA_G
Cuts at: 0 805 813 1380
Size: 805 8 567
Fragments arranged by size:
805 567 8
SmlI C'TyrA_G
Cuts at: 0 166 1380
Size: 166 1214
StuI AGG'CCT
Cuts at: 0 811 1380
Size: 811 569
StyI C'CwwG_G
Cuts at: 0 578 851 1380
Size: 578 273 529
Fragments arranged by size:
578 529 273
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1086 1118 1380
Size: 1086 32 262
Fragments arranged by size:
1086 262 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1027 1380
Size: 1027 353
XmnI GAAnn'nnTTC
Cuts at: 0 1060 1380
Size: 1060 320
Enzymes that do cut:
AarI Acc65I AcuI AlwNI ApaI ApaLI AvaI BanI
BanII BbsI BbvCI BclI BlpI Bme1580I BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaXI
BseRI BseYI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrBI
BsrDI BstXI BstYI BtgI BtsI CspCI EaeI EarI
EcoICRI Eco57MI EcoO109I HaeII Hin4I HincII KpnI MscI
MslI MspA1I NcoI NheI PfoI PpuMI PspOMI PstI
PvuII SacI SanDI SexAI SfcI SmlI StuI StyI
TstI XcmI XmnI
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AflIII AgeI AhdI
AleI AlfI AloI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BcgI BciVI BfrBI BglI BglII
BmgBI BsaBI BsaWI BsgI BsiEI BsiWI BsmI BspEI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I
Bsu36I BtgZI ClaI DraI DraIII DrdI EagI EciI
EcoNI EcoRI EcoRV FalI FseI FspI FspAI HindIII
HpaI KasI MfeI MluI MmeI NaeI NarI NdeI
NgoMIV NotI NruI NsiI NspI PacI PasI PciI
PflMI PmeI PmlI PpiI PshAI PsiI PspXI PsrI
PvuI RsrII SacII SalI SapI SbfI ScaI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI SwaI TaqII TatI Tth111I XbaI XhoI XmaI
ZraI