Restriction map for
pF1KB5334
(Linear) (Six-base) MAPSORT of: KIBB5334.seq Check: 8070 from: 1 to: 1566
KIBB5334.orf
With 182 enzymes: SgfI *
February 28, 2007 11:27 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 43 886 1566
Size: 43 843 680
Fragments arranged by size:
843 680 43
AleI CACnn'nnGTG
Cuts at: 0 754 1110 1566
Size: 754 356 456
Fragments arranged by size:
754 456 356
AlwNI CAG_nnn'CTG
Cuts at: 0 99 1566
Size: 99 1467
ApaLI G'TGCA_C
Cuts at: 0 105 1566
Size: 105 1461
ApoI r'AATT_y
Cuts at: 0 432 1566
Size: 432 1134
AvaI C'yCGr_G
Cuts at: 0 49 1566
Size: 49 1517
AvrII C'CTAG_G
Cuts at: 0 1291 1566
Size: 1291 275
BamHI G'GATC_C
Cuts at: 0 159 1566
Size: 159 1407
BanI G'GyrC_C
Cuts at: 0 45 646 1566
Size: 45 601 920
Fragments arranged by size:
920 601 45
BciVI GTATCCnnnnn_n'
Cuts at: 0 950 1566
Size: 950 616
BfrBI ATG'CAT
Cuts at: 0 717 1566
Size: 717 849
BglII A'GATC_T
Cuts at: 0 897 1566
Size: 897 669
Bme1580I G_kGCm'C
Cuts at: 0 50 109 481 649 1566
Size: 50 59 372 168 917
Fragments arranged by size:
917 372 168 59 50
BmrI ACTGGGnnnn_n'
Cuts at: 0 381 1566
Size: 381 1185
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 327 753 1566
Size: 327 426 813
Fragments arranged by size:
813 426 327
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 61 191 277 1304 1566
Size: 61 130 86 1027 262
Fragments arranged by size:
1027 262 130 86 61
BsaI GGTCTCn'nnnn_
Cuts at: 0 175 544 1367 1428 1566
Size: 175 369 823 61 138
Fragments arranged by size:
823 369 175 138 61
BsaHI Gr'CG_yC
Cuts at: 0 1411 1566
Size: 1411 155
BseYI C'CCAG_C
Cuts at: 0 66 1414 1525 1566
Size: 66 1348 111 41
Fragments arranged by size:
1348 111 66 41
BsiHKAI G_wGCw'C
Cuts at: 0 109 1566
Size: 109 1457
BsmI GAATG_Cn'
Cuts at: 0 282 540 1566
Size: 282 258 1026
Fragments arranged by size:
1026 282 258
BsmBI CGTCTCn'nnnn_
Cuts at: 0 46 1402 1566
Size: 46 1356 164
Fragments arranged by size:
1356 164 46
Bsp1286I G_dGCh'C
Cuts at: 0 50 109 481 649 1566
Size: 50 59 372 168 917
Fragments arranged by size:
917 372 168 59 50
BsrBI CCG'CTC
Cuts at: 0 692 1566
Size: 692 874
BsrFI r'CCGG_y
Cuts at: 0 1550 1566
Size: 1550 16
BsrGI T'GTAC_A
Cuts at: 0 1184 1566
Size: 1184 382
BstAPI GCAn_nnn'nTGC
Cuts at: 0 99 714 1566
Size: 99 615 852
Fragments arranged by size:
852 615 99
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1112 1566
Size: 1112 454
BstYI r'GATC_y
Cuts at: 0 159 897 1025 1566
Size: 159 738 128 541
Fragments arranged by size:
738 541 159 128
Bsu36I CC'TnA_GG
Cuts at: 0 88 641 1566
Size: 88 553 925
Fragments arranged by size:
925 553 88
DraI TTT'AAA
Cuts at: 0 924 1566
Size: 924 642
DrdI GACnn_nn'nnGTC
Cuts at: 0 1150 1566
Size: 1150 416
EaeI y'GGCC_r
Cuts at: 0 34 317 1537 1548 1566
Size: 34 283 1220 11 18
Fragments arranged by size:
1220 283 34 18 11
EarI CTCTTCn'nnn_
Cuts at: 0 173 1186 1566
Size: 173 1013 380
Fragments arranged by size:
1013 380 173
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 43 61 191 277 886 1304 1566
Size: 43 18 130 86 609 418 262
Fragments arranged by size:
609 418 262 130 86 43 18
EcoO109I rG'GnC_Cy
Cuts at: 0 421 1468 1566
Size: 421 1047 98
Fragments arranged by size:
1047 421 98
FspI TGC'GCA
Cuts at: 0 464 1566
Size: 464 1102
FspAI rTGC'GCAy
Cuts at: 0 464 1566
Size: 464 1102
HaeII r_GCGC'y
Cuts at: 0 253 1566
Size: 253 1313
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 543 575 1364 1396 1566
Size: 543 32 789 32 170
Fragments arranged by size:
789 543 170 32 32
HincII GTy'rAC
Cuts at: 0 1253 1566
Size: 1253 313
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 602 1056 1350 1566
Size: 602 454 294 216
Fragments arranged by size:
602 454 294 216
MscI TGG'CCA
Cuts at: 0 319 1566
Size: 319 1247
MslI CAynn'nnrTG
Cuts at: 0 754 1110 1566
Size: 754 356 456
Fragments arranged by size:
754 456 356
MspA1I CmG'CkG
Cuts at: 0 39 568 834 933 1566
Size: 39 529 266 99 633
Fragments arranged by size:
633 529 266 99 39
NaeI GCC'GGC
Cuts at: 0 1552 1566
Size: 1552 14
NdeI CA'TA_TG
Cuts at: 0 195 467 1566
Size: 195 272 1099
Fragments arranged by size:
1099 272 195
NgoMIV G'CCGG_C
Cuts at: 0 1550 1566
Size: 1550 16
NsiI A_TGCA'T
Cuts at: 0 719 1566
Size: 719 847
NspI r_CATG'y
Cuts at: 0 717 1566
Size: 717 849
PflMI CCAn_nnn'nTGG
Cuts at: 0 1546 1566
Size: 1546 20
PfoI T'CCnGG_A
Cuts at: 0 1320 1464 1566
Size: 1320 144 102
Fragments arranged by size:
1320 144 102
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 491 523 1566
Size: 491 32 1043
Fragments arranged by size:
1043 491 32
PpuMI rG'GwC_Cy
Cuts at: 0 1468 1566
Size: 1468 98
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1004 1036 1566
Size: 1004 32 530
Fragments arranged by size:
1004 530 32
PstI C_TGCA'G
Cuts at: 0 710 1566
Size: 710 856
PvuII CAG'CTG
Cuts at: 0 568 834 933 1566
Size: 568 266 99 633
Fragments arranged by size:
633 568 266 99
SfcI C'TryA_G
Cuts at: 0 510 706 1566
Size: 510 196 860
Fragments arranged by size:
860 510 196
StyI C'CwwG_G
Cuts at: 0 185 372 1291 1566
Size: 185 187 919 275
Fragments arranged by size:
919 275 187 185
TatI w'GTAC_w
Cuts at: 0 710 1184 1566
Size: 710 474 382
Fragments arranged by size:
710 474 382
Enzymes that do cut:
AcuI AleI AlwNI ApaLI ApoI AvaI AvrII BamHI
BanI BciVI BfrBI BglII Bme1580I BmrI BplI BpmI
BsaI BsaHI BseYI BsiHKAI BsmI BsmBI Bsp1286I BsrBI
BsrFI BsrGI BstAPI BstXI BstYI Bsu36I DraI DrdI
EaeI EarI Eco57MI EcoO109I FspI FspAI HaeII Hin4I
HincII MmeI MscI MslI MspA1I NaeI NdeI NgoMIV
NsiI NspI PflMI PfoI PpiI PpuMI PsrI PstI
PvuII SfcI StyI TatI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AlfI AloI ApaI AscI AseI AsiSI
BaeI BanII BbeI BbsI BbvCI BcgI BclI BglI
BlpI BmgBI BmtI Bpu10I BpuEI BsaAI BsaBI BsaWI
BsaXI BseRI BsgI BsiEI BsiWI BspEI BspHI BspMI
BsrDI BssHII BssSI BstBI BstEII BstZ17I BtgI BtgZI
BtsI ClaI CspCI DraIII EagI EciI EcoICRI EcoNI
EcoRI EcoRV FalI FseI HindIII HpaI KasI KpnI
MfeI MluI NarI NcoI NheI NotI NruI PacI
PasI PciI PmeI PmlI PshAI PsiI PspOMI PspXI
PvuI RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI