Restriction map for
pF1KB5335
(Linear) (Six-base) MAPSORT of: KIBB5335.seq Check: 5319 from: 1 to: 1959
KIBB5335.orf
With 182 enzymes: SgfI *
February 28, 2007 11:27 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 648 732 900 984 1068 1152 1236 1404
Size: 648 84 168 84 84 84 84 168
Cuts at: 1404 1488 1824 1959
Size: 84 336 135
Fragments arranged by size:
648 336 168 168 135 84 84 84
84 84 84
AflIII A'CryG_T
Cuts at: 0 722 890 974 1646 1825 1959
Size: 722 168 84 672 179 134
Fragments arranged by size:
722 672 179 168 134 84
AleI CACnn'nnGTG
Cuts at: 0 875 1959
Size: 875 1084
AlwNI CAG_nnn'CTG
Cuts at: 0 1070 1959
Size: 1070 889
ApaI G_GGCC'C
Cuts at: 0 367 1959
Size: 367 1592
ApoI r'AATT_y
Cuts at: 0 122 274 1260 1959
Size: 122 152 986 699
Fragments arranged by size:
986 699 152 122
AvaI C'yCGr_G
Cuts at: 0 789 1629 1959
Size: 789 840 330
Fragments arranged by size:
840 789 330
BamHI G'GATC_C
Cuts at: 0 357 1959
Size: 357 1602
BanI G'GyrC_C
Cuts at: 0 445 1959
Size: 445 1514
BanII G_rGCy'C
Cuts at: 0 58 233 367 1710 1959
Size: 58 175 134 1343 249
Fragments arranged by size:
1343 249 175 134 58
BbsI GAAGACnn'nnnn_
Cuts at: 0 1057 1879 1959
Size: 1057 822 80
Fragments arranged by size:
1057 822 80
BbvCI CC'TCA_GC
Cuts at: 0 1858 1959
Size: 1858 101
BciVI GTATCCnnnnn_n'
Cuts at: 0 692 1959
Size: 692 1267
BclI T'GATC_A
Cuts at: 0 417 1959
Size: 417 1542
BlpI GC'TnA_GC
Cuts at: 0 52 1959
Size: 52 1907
Bme1580I G_kGCm'C
Cuts at: 0 367 1247 1959
Size: 367 880 712
Fragments arranged by size:
880 712 367
BmgBI CAC'GTC
Cuts at: 0 1826 1880 1959
Size: 1826 54 79
Fragments arranged by size:
1826 79 54
BmrI ACTGGGnnnn_n'
Cuts at: 0 127 1304 1675 1959
Size: 127 1177 371 284
Fragments arranged by size:
1177 371 284 127
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 77 1959
Size: 77 1882
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 112 178 198 249 432 480 1959
Size: 112 66 20 51 183 48 1479
Fragments arranged by size:
1479 183 112 66 51 48 20
Bpu10I CC'TnA_GC
Cuts at: 0 1858 1916 1959
Size: 1858 58 43
Fragments arranged by size:
1858 58 43
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1327 1831 1959
Size: 1327 504 128
Fragments arranged by size:
1327 504 128
BsaI GGTCTCn'nnnn_
Cuts at: 0 117 166 399 1609 1959
Size: 117 49 233 1210 350
Fragments arranged by size:
1210 350 233 117 49
BsaAI yAC'GTr
Cuts at: 0 723 975 1959
Size: 723 252 984
Fragments arranged by size:
984 723 252
BsaWI w'CCGG_w
Cuts at: 0 836 874 1959
Size: 836 38 1085
Fragments arranged by size:
1085 836 38
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1090 1120 1291 1321 1543 1573 1959
Size: 1090 30 171 30 222 30 386
Fragments arranged by size:
1090 386 222 171 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1060 1959
Size: 1060 899
BseYI C'CCAG_C
Cuts at: 0 152 564 1959
Size: 152 412 1395
Fragments arranged by size:
1395 412 152
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 353 831 915 1083 1671 1755 1924 1946
Size: 353 478 84 168 588 84 169 22
Cuts at: 1946 1959
Size: 13
Fragments arranged by size:
588 478 353 169 168 84 84 22
13
BsiHKAI G_wGCw'C
Cuts at: 0 233 1959
Size: 233 1726
BsmI GAATG_Cn'
Cuts at: 0 4 1959
Size: 4 1955
Bsp1286I G_dGCh'C
Cuts at: 0 58 233 367 1247 1710 1959
Size: 58 175 134 880 463 249
Fragments arranged by size:
880 463 249 175 134 58
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 689 1959
Size: 689 1270
BsrFI r'CCGG_y
Cuts at: 0 201 1600 1959
Size: 201 1399 359
Fragments arranged by size:
1399 359 201
BsrGI T'GTAC_A
Cuts at: 0 74 1959
Size: 74 1885
BstAPI GCAn_nnn'nTGC
Cuts at: 0 291 1959
Size: 291 1668
BstYI r'GATC_y
Cuts at: 0 216 314 357 1959
Size: 216 98 43 1602
Fragments arranged by size:
1602 216 98 43
Bsu36I CC'TnA_GG
Cuts at: 0 434 584 1959
Size: 434 150 1375
Fragments arranged by size:
1375 434 150
BtgI C'CryG_G
Cuts at: 0 1475 1959
Size: 1475 484
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1176 1959
Size: 1176 783
BtsI GCAGTG_nn'
Cuts at: 0 903 1071 1663 1959
Size: 903 168 592 296
Fragments arranged by size:
903 592 296 168
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1137 1172 1389 1424 1809 1844 1959
Size: 1137 35 217 35 385 35 115
Fragments arranged by size:
1137 385 217 115 35 35 35
EaeI y'GGCC_r
Cuts at: 0 1410 1746 1959
Size: 1410 336 213
Fragments arranged by size:
1410 336 213
EarI CTCTTCn'nnn_
Cuts at: 0 601 754 1712 1959
Size: 601 153 958 247
Fragments arranged by size:
958 601 247 153
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1299 1959
Size: 1299 660
EcoICRI GAG'CTC
Cuts at: 0 231 1959
Size: 231 1728
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 112 178 198 249 432 480 648 732
Size: 112 66 20 51 183 48 168 84
Cuts at: 732 900 984 1068 1152 1236 1404 1488 1824
Size: 168 84 84 84 84 168 84 336
Cuts at: 1824 1959
Size: 135
Fragments arranged by size:
336 183 168 168 168 135 112 84
84 84 84 84 84 66 51 48
20
EcoO109I rG'GnC_Cy
Cuts at: 0 430 1959
Size: 430 1529
EcoRI G'AATT_C
Cuts at: 0 1260 1959
Size: 1260 699
HincII GTy'rAC
Cuts at: 0 639 845 1191 1959
Size: 639 206 346 768
Fragments arranged by size:
768 639 346 206
MfeI C'AATT_G
Cuts at: 0 803 1959
Size: 803 1156
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1457 1959
Size: 1457 502
MscI TGG'CCA
Cuts at: 0 1748 1959
Size: 1748 211
MslI CAynn'nnrTG
Cuts at: 0 327 875 1815 1959
Size: 327 548 940 144
Fragments arranged by size:
940 548 327 144
MspA1I CmG'CkG
Cuts at: 0 156 696 1015 1959
Size: 156 540 319 944
Fragments arranged by size:
944 540 319 156
NcoI C'CATG_G
Cuts at: 0 1475 1959
Size: 1475 484
NdeI CA'TA_TG
Cuts at: 0 1812 1959
Size: 1812 147
NspI r_CATG'y
Cuts at: 0 292 326 894 1650 1959
Size: 292 34 568 756 309
Fragments arranged by size:
756 568 309 292 34
PasI CC'CwG_GG
Cuts at: 0 367 1959
Size: 367 1592
PciI A'CATG_T
Cuts at: 0 890 1646 1959
Size: 890 756 313
Fragments arranged by size:
890 756 313
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1360 1392 1959
Size: 1360 32 567
Fragments arranged by size:
1360 567 32
PpuMI rG'GwC_Cy
Cuts at: 0 430 1959
Size: 430 1529
PspOMI G'GGCC_C
Cuts at: 0 363 1959
Size: 363 1596
PspXI vC'TCGA_Gb
Cuts at: 0 1629 1959
Size: 1629 330
PvuII CAG'CTG
Cuts at: 0 156 1015 1959
Size: 156 859 944
Fragments arranged by size:
944 859 156
SacI G_AGCT'C
Cuts at: 0 233 1959
Size: 233 1726
SapI GCTCTTCn'nnn_
Cuts at: 0 754 1959
Size: 754 1205
SexAI A'CCwGG_T
Cuts at: 0 107 1959
Size: 107 1852
SfcI C'TryA_G
Cuts at: 0 99 1959
Size: 99 1860
SmlI C'TyrA_G
Cuts at: 0 1342 1629 1846 1959
Size: 1342 287 217 113
Fragments arranged by size:
1342 287 217 113
StyI C'CwwG_G
Cuts at: 0 1475 1749 1959
Size: 1475 274 210
Fragments arranged by size:
1475 274 210
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 46 166 1959
Size: 46 120 1793
Fragments arranged by size:
1793 120 46
TatI w'GTAC_w
Cuts at: 0 74 1959
Size: 74 1885
Tth111I GACn'n_nGTC
Cuts at: 0 1878 1959
Size: 1878 81
XhoI C'TCGA_G
Cuts at: 0 1629 1959
Size: 1629 330
XmnI GAAnn'nnTTC
Cuts at: 0 745 1959
Size: 745 1214
Enzymes that do cut:
AcuI AflIII AleI AlwNI ApaI ApoI AvaI BamHI
BanI BanII BbsI BbvCI BciVI BclI BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I
BspMI BsrFI BsrGI BstAPI BstYI Bsu36I BtgI BtgZI
BtsI CspCI EaeI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI HincII MfeI MmeI MscI MslI MspA1I NcoI
NdeI NspI PasI PciI PpiI PpuMI PspOMI PspXI
PvuII SacI SapI SexAI SfcI SmlI StyI TaqII
TatI Tth111I XhoI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AlfI AloI ApaLI AscI AseI AsiSI AvrII
BaeI BbeI BcgI BfrBI BglI BglII BmtI BsaBI
BsaHI BsiEI BsiWI BsmBI BspEI BspHI BsrBI BsrDI
BssHII BssSI BstBI BstEII BstXI BstZ17I ClaI DraI
DraIII DrdI EagI EcoNI EcoRV FalI FseI FspI
FspAI HaeII Hin4I HindIII HpaI KasI KpnI MluI
NaeI NarI NgoMIV NheI NotI NruI NsiI PacI
PflMI PfoI PmeI PmlI PshAI PsiI PsrI PstI
PvuI RsrII SacII SalI SanDI SbfI ScaI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI StuI SwaI TstI XbaI XcmI XmaI ZraI