Restriction map for
pF1KB5337
(Linear) (Six-base) MAPSORT of: KIBB5337.seq Check: 2392 from: 1 to: 774
KIBB5337.orf
With 182 enzymes: SgfI *
February 20, 2009 16:16 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 398 774
Size: 398 376
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 366 774
Size: 366 408
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 430 774
Size: 430 344
AlwNI CAG_nnn'CTG
Cuts at: 0 389 774
Size: 389 385
ApaI G_GGCC'C
Cuts at: 0 47 774
Size: 47 727
ApoI r'AATT_y
Cuts at: 0 434 774
Size: 434 340
AvaI C'yCGr_G
Cuts at: 0 46 225 248 485 774
Size: 46 179 23 237 289
Fragments arranged by size:
289 237 179 46 23
BanI G'GyrC_C
Cuts at: 0 410 480 774
Size: 410 70 294
Fragments arranged by size:
410 294 70
BanII G_rGCy'C
Cuts at: 0 47 517 774
Size: 47 470 257
Fragments arranged by size:
470 257 47
BbeI G_GCGC'C
Cuts at: 0 484 774
Size: 484 290
BbsI GAAGACnn'nnnn_
Cuts at: 0 700 774
Size: 700 74
Bme1580I G_kGCm'C
Cuts at: 0 47 399 509 774
Size: 47 352 110 265
Fragments arranged by size:
352 265 110 47
BmtI G_CTAG'C
Cuts at: 0 106 774
Size: 106 668
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 565 742 774
Size: 565 177 32
Fragments arranged by size:
565 177 32
BsaI GGTCTCn'nnnn_
Cuts at: 0 434 774
Size: 434 340
BsaHI Gr'CG_yC
Cuts at: 0 481 774
Size: 481 293
BsaWI w'CCGG_w
Cuts at: 0 443 689 774
Size: 443 246 85
Fragments arranged by size:
443 246 85
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 438 468 774
Size: 438 30 306
Fragments arranged by size:
438 306 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 109 148 774
Size: 109 39 626
Fragments arranged by size:
626 109 39
BseYI C'CCAG_C
Cuts at: 0 648 774
Size: 648 126
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 253 517 774
Size: 253 264 257
Fragments arranged by size:
264 257 253
BsiEI CG_ry'CG
Cuts at: 0 615 774
Size: 615 159
BsiHKAI G_wGCw'C
Cuts at: 0 451 774
Size: 451 323
Bsp1286I G_dGCh'C
Cuts at: 0 47 399 451 509 517 774
Size: 47 352 52 58 8 257
Fragments arranged by size:
352 257 58 52 47 8
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 398 774
Size: 398 376
BsrBI CCG'CTC
Cuts at: 0 549 774
Size: 549 225
BsrGI T'GTAC_A
Cuts at: 0 206 774
Size: 206 568
BssHII G'CGCG_C
Cuts at: 0 106 774
Size: 106 668
BssSI C'ACGA_G
Cuts at: 0 16 577 774
Size: 16 561 197
Fragments arranged by size:
561 197 16
BstEII G'GTnAC_C
Cuts at: 0 361 774
Size: 361 413
BtgI C'CryG_G
Cuts at: 0 122 774
Size: 122 652
BtsI GCAGTG_nn'
Cuts at: 0 75 646 774
Size: 75 571 128
Fragments arranged by size:
571 128 75
EaeI y'GGCC_r
Cuts at: 0 20 35 554 774
Size: 20 15 519 220
Fragments arranged by size:
519 220 20 15
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 325 774
Size: 325 449
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 366 565 742 774
Size: 366 199 177 32
Fragments arranged by size:
366 199 177 32
EcoO109I rG'GnC_Cy
Cuts at: 0 582 717 774
Size: 582 135 57
Fragments arranged by size:
582 135 57
HaeII r_GCGC'y
Cuts at: 0 484 544 774
Size: 484 60 230
Fragments arranged by size:
484 230 60
HindIII A'AGCT_T
Cuts at: 0 706 774
Size: 706 68
KasI G'GCGC_C
Cuts at: 0 480 774
Size: 480 294
MslI CAynn'nnrTG
Cuts at: 0 277 774
Size: 277 497
MspA1I CmG'CkG
Cuts at: 0 753 774
Size: 753 21
NarI GG'CG_CC
Cuts at: 0 481 774
Size: 481 293
NheI G'CTAG_C
Cuts at: 0 102 774
Size: 102 672
PasI CC'CwG_GG
Cuts at: 0 180 510 774
Size: 180 330 264
Fragments arranged by size:
330 264 180
PflMI CCAn_nnn'nTGG
Cuts at: 0 753 774
Size: 753 21
PfoI T'CCnGG_A
Cuts at: 0 326 774
Size: 326 448
PpuMI rG'GwC_Cy
Cuts at: 0 582 774
Size: 582 192
PspOMI G'GGCC_C
Cuts at: 0 43 774
Size: 43 731
PstI C_TGCA'G
Cuts at: 0 341 419 774
Size: 341 78 355
Fragments arranged by size:
355 341 78
PvuII CAG'CTG
Cuts at: 0 753 774
Size: 753 21
SfcI C'TryA_G
Cuts at: 0 337 390 415 774
Size: 337 53 25 359
Fragments arranged by size:
359 337 53 25
SfoI GGC'GCC
Cuts at: 0 482 774
Size: 482 292
SmaI CCC'GGG
Cuts at: 0 48 487 774
Size: 48 439 287
Fragments arranged by size:
439 287 48
SrfI GCCC'GGGC
Cuts at: 0 48 774
Size: 48 726
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 589 662 774
Size: 589 73 112
Fragments arranged by size:
589 112 73
TatI w'GTAC_w
Cuts at: 0 206 774
Size: 206 568
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 438 470 774
Size: 438 32 304
Fragments arranged by size:
438 304 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 177 774
Size: 177 597
XmaI C'CCGG_G
Cuts at: 0 46 485 774
Size: 46 439 289
Fragments arranged by size:
439 289 46
XmnI GAAnn'nnTTC
Cuts at: 0 424 774
Size: 424 350
Enzymes that do cut:
AarI AcuI AlfI AlwNI ApaI ApoI AvaI BanI
BanII BbeI BbsI Bme1580I BmtI BpmI BsaI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspMI BsrBI BsrGI BssHII BssSI BstEII BtgI BtsI
EaeI EciI Eco57MI EcoO109I HaeII HindIII KasI MslI
MspA1I NarI NheI PasI PflMI PfoI PpuMI PspOMI
PstI PvuII SfcI SfoI SmaI SrfI TaqII TatI
TstI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AloI ApaLI AscI AseI AsiSI AvrII
BaeI BamHI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI BmgBI BmrI BplI Bpu10I BpuEI BsaAI
BsaBI BsiWI BsmI BsmBI BspEI BspHI BsrDI BsrFI
BstAPI BstBI BstXI BstYI BstZ17I Bsu36I BtgZI ClaI
CspCI DraI DraIII DrdI EagI EarI EcoICRI EcoNI
EcoRI EcoRV FalI FseI FspI FspAI Hin4I HincII
HpaI KpnI MfeI MluI MmeI MscI NaeI NcoI
NdeI NgoMIV NotI NruI NsiI NspI PacI PciI
PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfiI SgfI SgrAI SmlI SnaBI SpeI SphI
SspI StuI StyI SwaI Tth111I XbaI XhoI ZraI