Restriction map for
pF1KB5339
(Linear) (Six-base) MAPSORT of: KIBB5339.seq Check: 2787 from: 1 to: 1602
KIBB5339.orf
With 182 enzymes: SgfI *
February 28, 2007 11:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 454 1602
Size: 454 1148
Acc65I G'GTAC_C
Cuts at: 0 231 1602
Size: 231 1371
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 545 752 1246 1565 1602
Size: 545 207 494 319 37
Fragments arranged by size:
545 494 319 207 37
AflIII A'CryG_T
Cuts at: 0 366 1602
Size: 366 1236
AleI CACnn'nnGTG
Cuts at: 0 760 1602
Size: 760 842
AlwNI CAG_nnn'CTG
Cuts at: 0 1140 1602
Size: 1140 462
ApoI r'AATT_y
Cuts at: 0 408 427 508 1066 1285 1450 1602
Size: 408 19 81 558 219 165 152
Fragments arranged by size:
558 408 219 165 152 81 19
BanI G'GyrC_C
Cuts at: 0 6 231 1602
Size: 6 225 1371
Fragments arranged by size:
1371 225 6
BanII G_rGCy'C
Cuts at: 0 636 1602
Size: 636 966
BbeI G_GCGC'C
Cuts at: 0 10 1602
Size: 10 1592
BfrBI ATG'CAT
Cuts at: 0 774 1602
Size: 774 828
BlpI GC'TnA_GC
Cuts at: 0 324 1602
Size: 324 1278
BmrI ACTGGGnnnn_n'
Cuts at: 0 307 1602
Size: 307 1295
BmtI G_CTAG'C
Cuts at: 0 616 1602
Size: 616 986
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 208 1602
Size: 208 1394
BsaHI Gr'CG_yC
Cuts at: 0 7 22 1156 1602
Size: 7 15 1134 446
Fragments arranged by size:
1134 446 15 7
BsaWI w'CCGG_w
Cuts at: 0 356 1602
Size: 356 1246
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1190 1220 1602
Size: 1190 30 382
Fragments arranged by size:
1190 382 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 118 1326 1602
Size: 118 1208 276
Fragments arranged by size:
1208 276 118
BseYI C'CCAG_C
Cuts at: 0 669 1479 1602
Size: 669 810 123
Fragments arranged by size:
810 669 123
BsiHKAI G_wGCw'C
Cuts at: 0 325 356 1602
Size: 325 31 1246
Fragments arranged by size:
1246 325 31
BsmI GAATG_Cn'
Cuts at: 0 543 771 1602
Size: 543 228 831
Fragments arranged by size:
831 543 228
BsmBI CGTCTCn'nnnn_
Cuts at: 0 13 1602
Size: 13 1589
Bsp1286I G_dGCh'C
Cuts at: 0 325 356 636 1602
Size: 325 31 280 966
Fragments arranged by size:
966 325 280 31
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 454 1602
Size: 454 1148
BsrDI GCAATG_nn'
Cuts at: 0 1484 1602
Size: 1484 118
BssHII G'CGCG_C
Cuts at: 0 75 1602
Size: 75 1527
BstAPI GCAn_nnn'nTGC
Cuts at: 0 583 1602
Size: 583 1019
BstBI TT'CG_AA
Cuts at: 0 506 1602
Size: 506 1096
Bsu36I CC'TnA_GG
Cuts at: 0 1418 1602
Size: 1418 184
BtgI C'CryG_G
Cuts at: 0 14 1602
Size: 14 1588
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 470 784 1602
Size: 470 314 818
Fragments arranged by size:
818 470 314
BtsI GCAGTG_nn'
Cuts at: 0 1125 1281 1347 1602
Size: 1125 156 66 255
Fragments arranged by size:
1125 255 156 66
DraI TTT'AAA
Cuts at: 0 513 1064 1575 1602
Size: 513 551 511 27
Fragments arranged by size:
551 513 511 27
EaeI y'GGCC_r
Cuts at: 0 1122 1602
Size: 1122 480
EarI CTCTTCn'nnn_
Cuts at: 0 728 1361 1602
Size: 728 633 241
Fragments arranged by size:
728 633 241
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 545 752 1246 1565 1602
Size: 545 207 494 319 37
Fragments arranged by size:
545 494 319 207 37
EcoRI G'AATT_C
Cuts at: 0 427 1602
Size: 427 1175
HaeII r_GCGC'y
Cuts at: 0 10 1602
Size: 10 1592
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 199 231 583 615 709 741 1190 1222
Size: 199 32 352 32 94 32 449 32
Cuts at: 1222 1602
Size: 380
Fragments arranged by size:
449 380 352 199 94 32 32 32
32
HincII GTy'rAC
Cuts at: 0 477 1602
Size: 477 1125
KasI G'GCGC_C
Cuts at: 0 6 1602
Size: 6 1596
KpnI G_GTAC'C
Cuts at: 0 235 1602
Size: 235 1367
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 957 1514 1602
Size: 957 557 88
Fragments arranged by size:
957 557 88
MscI TGG'CCA
Cuts at: 0 1124 1602
Size: 1124 478
MslI CAynn'nnrTG
Cuts at: 0 278 760 809 893 1602
Size: 278 482 49 84 709
Fragments arranged by size:
709 482 278 84 49
MspA1I CmG'CkG
Cuts at: 0 1322 1602
Size: 1322 280
NarI GG'CG_CC
Cuts at: 0 7 1602
Size: 7 1595
NheI G'CTAG_C
Cuts at: 0 612 1602
Size: 612 990
NsiI A_TGCA'T
Cuts at: 0 776 1602
Size: 776 826
NspI r_CATG'y
Cuts at: 0 370 1602
Size: 370 1232
PciI A'CATG_T
Cuts at: 0 366 1602
Size: 366 1236
PflMI CCAn_nnn'nTGG
Cuts at: 0 835 1602
Size: 835 767
PsiI TTA'TAA
Cuts at: 0 221 1602
Size: 221 1381
PstI C_TGCA'G
Cuts at: 0 465 1164 1602
Size: 465 699 438
Fragments arranged by size:
699 465 438
PvuII CAG'CTG
Cuts at: 0 1322 1602
Size: 1322 280
SfcI C'TryA_G
Cuts at: 0 338 461 1160 1602
Size: 338 123 699 442
Fragments arranged by size:
699 442 338 123
SfoI GGC'GCC
Cuts at: 0 8 1602
Size: 8 1594
SpeI A'CTAG_T
Cuts at: 0 1264 1602
Size: 1264 338
StyI C'CwwG_G
Cuts at: 0 602 1602
Size: 602 1000
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 661 1602
Size: 661 941
Enzymes that do cut:
AarI Acc65I AcuI AflIII AleI AlwNI ApoI BanI
BanII BbeI BfrBI BlpI BmrI BmtI BplI BsaHI
BsaWI BsaXI BseRI BseYI BsiHKAI BsmI BsmBI Bsp1286I
BspMI BsrDI BssHII BstAPI BstBI Bsu36I BtgI BtgZI
BtsI DraI EaeI EarI Eco57MI EcoRI HaeII Hin4I
HincII KasI KpnI MmeI MscI MslI MspA1I NarI
NheI NsiI NspI PciI PflMI PsiI PstI PvuII
SfcI SfoI SpeI StyI TaqII
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AgeI AhdI AlfI
AloI ApaI ApaLI AscI AseI AsiSI AvaI AvrII
BaeI BamHI BbsI BbvCI BcgI BciVI BclI BglI
BglII Bme1580I BmgBI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsgI BsiEI BsiWI BspEI BspHI BsrBI BsrFI
BsrGI BssSI BstEII BstXI BstYI BstZ17I ClaI CspCI
DraIII DrdI EagI EciI EcoICRI EcoNI EcoO109I EcoRV
FalI FseI FspI FspAI HindIII HpaI MfeI MluI
NaeI NcoI NdeI NgoMIV NotI NruI PacI PasI
PfoI PmeI PmlI PpiI PpuMI PshAI PspOMI PspXI
PsrI PvuI RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfiI SgfI SgrAI SmaI SmlI
SnaBI SphI SrfI SspI StuI SwaI TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI