Restriction map for
pF1KB5343
(Linear) (Six-base) MAPSORT of: KIBB5343.seq Check: 3539 from: 1 to: 1764
KIBB5343.orf
With 182 enzymes: SgfI *
July 18, 2007 13:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1157 1764
Size: 1157 607
AatII G_ACGT'C
Cuts at: 0 1103 1764
Size: 1103 661
AccI GT'mk_AC
Cuts at: 0 1133 1764
Size: 1133 631
AclI AA'CG_TT
Cuts at: 0 187 1764
Size: 187 1577
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 139 1009 1339 1764
Size: 139 870 330 425
Fragments arranged by size:
870 425 330 139
AflIII A'CryG_T
Cuts at: 0 794 1764
Size: 794 970
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1286 1318 1764
Size: 1286 32 446
Fragments arranged by size:
1286 446 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1456 1569 1764
Size: 1456 113 195
Fragments arranged by size:
1456 195 113
AvaI C'yCGr_G
Cuts at: 0 748 1764
Size: 748 1016
BanI G'GyrC_C
Cuts at: 0 787 861 1011 1540 1764
Size: 787 74 150 529 224
Fragments arranged by size:
787 529 224 150 74
BbsI GAAGACnn'nnnn_
Cuts at: 0 534 938 1764
Size: 534 404 826
Fragments arranged by size:
826 534 404
BciVI GTATCCnnnnn_n'
Cuts at: 0 380 1764
Size: 380 1384
BglI GCCn_nnn'nGGC
Cuts at: 0 847 1486 1750 1764
Size: 847 639 264 14
Fragments arranged by size:
847 639 264 14
BlpI GC'TnA_GC
Cuts at: 0 85 1764
Size: 85 1679
Bme1580I G_kGCm'C
Cuts at: 0 531 790 1016 1764
Size: 531 259 226 748
Fragments arranged by size:
748 531 259 226
BmgBI CAC'GTC
Cuts at: 0 642 1764
Size: 642 1122
BmrI ACTGGGnnnn_n'
Cuts at: 0 592 1179 1764
Size: 592 587 585
Fragments arranged by size:
592 587 585
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1257 1764
Size: 1257 507
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 64 970 1573 1764
Size: 64 906 603 191
Fragments arranged by size:
906 603 191 64
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 31 1764
Size: 31 1733
BsaAI yAC'GTr
Cuts at: 0 462 1176 1764
Size: 462 714 588
Fragments arranged by size:
714 588 462
BsaHI Gr'CG_yC
Cuts at: 0 1100 1764
Size: 1100 664
BsaWI w'CCGG_w
Cuts at: 0 266 275 1764
Size: 266 9 1489
Fragments arranged by size:
1489 266 9
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1035 1065 1286 1316 1764
Size: 1035 30 221 30 448
Fragments arranged by size:
1035 448 221 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 208 889 892 1390 1393 1447 1450 1453
Size: 208 681 3 498 3 54 3 3
Cuts at: 1453 1594 1597 1764
Size: 141 3 167
Fragments arranged by size:
681 498 208 167 141 54 3 3
3 3 3
BseYI C'CCAG_C
Cuts at: 0 690 1764
Size: 690 1074
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 484 1198 1270 1764
Size: 484 714 72 494
Fragments arranged by size:
714 494 484 72
BsiEI CG_ry'CG
Cuts at: 0 1137 1764
Size: 1137 627
BsmI GAATG_Cn'
Cuts at: 0 113 1764
Size: 113 1651
Bsp1286I G_dGCh'C
Cuts at: 0 531 790 1016 1764
Size: 531 259 226 748
Fragments arranged by size:
748 531 259 226
BspEI T'CCGG_A
Cuts at: 0 266 275 1764
Size: 266 9 1489
Fragments arranged by size:
1489 266 9
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1157 1764
Size: 1157 607
BsrFI r'CCGG_y
Cuts at: 0 485 778 1136 1764
Size: 485 293 358 628
Fragments arranged by size:
628 485 358 293
BstXI CCAn_nnnn'nTGG
Cuts at: 0 690 1533 1602 1692 1764
Size: 690 843 69 90 72
Fragments arranged by size:
843 690 90 72 69
BtgI C'CryG_G
Cuts at: 0 442 1226 1764
Size: 442 784 538
Fragments arranged by size:
784 538 442
ClaI AT'CG_AT
Cuts at: 0 383 1764
Size: 383 1381
DraI TTT'AAA
Cuts at: 0 320 1764
Size: 320 1444
DrdI GACnn_nn'nnGTC
Cuts at: 0 652 1764
Size: 652 1112
EaeI y'GGCC_r
Cuts at: 0 38 659 1604 1764
Size: 38 621 945 160
Fragments arranged by size:
945 621 160 38
EarI CTCTTCn'nnn_
Cuts at: 0 286 1367 1379 1412 1469 1493 1764
Size: 286 1081 12 33 57 24 271
Fragments arranged by size:
1081 286 271 57 33 24 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 697 1711 1764
Size: 697 1014 53
Fragments arranged by size:
1014 697 53
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 64 139 970 1009 1339 1573 1764
Size: 64 75 831 39 330 234 191
Fragments arranged by size:
831 330 234 191 75 64 39
EcoNI CCTnn'n_nnAGG
Cuts at: 0 535 1764
Size: 535 1229
EcoO109I rG'GnC_Cy
Cuts at: 0 396 984 1023 1347 1764
Size: 396 588 39 324 417
Fragments arranged by size:
588 417 396 324 39
FspI TGC'GCA
Cuts at: 0 535 1764
Size: 535 1229
HaeII r_GCGC'y
Cuts at: 0 1602 1764
Size: 1602 162
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 397 429 1764
Size: 397 32 1335
Fragments arranged by size:
1335 397 32
HincII GTy'rAC
Cuts at: 0 162 237 702 1134 1764
Size: 162 75 465 432 630
Fragments arranged by size:
630 465 432 162 75
HindIII A'AGCT_T
Cuts at: 0 973 1764
Size: 973 791
HpaI GTT'AAC
Cuts at: 0 162 1764
Size: 162 1602
MluI A'CGCG_T
Cuts at: 0 794 1764
Size: 794 970
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 779 1764
Size: 779 985
MscI TGG'CCA
Cuts at: 0 661 1606 1764
Size: 661 945 158
Fragments arranged by size:
945 661 158
MslI CAynn'nnrTG
Cuts at: 0 1612 1764
Size: 1612 152
MspA1I CmG'CkG
Cuts at: 0 84 444 477 1254 1338 1566 1764
Size: 84 360 33 777 84 228 198
Fragments arranged by size:
777 360 228 198 84 84 33
NaeI GCC'GGC
Cuts at: 0 487 1764
Size: 487 1277
NgoMIV G'CCGG_C
Cuts at: 0 485 1764
Size: 485 1279
PasI CC'CwG_GG
Cuts at: 0 1526 1764
Size: 1526 238
PflMI CCAn_nnn'nTGG
Cuts at: 0 599 1764
Size: 599 1165
PmlI CAC'GTG
Cuts at: 0 462 1764
Size: 462 1302
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1286 1318 1764
Size: 1286 32 446
Fragments arranged by size:
1286 446 32
PpuMI rG'GwC_Cy
Cuts at: 0 396 1347 1764
Size: 396 951 417
Fragments arranged by size:
951 417 396
PshAI GACnn'nnGTC
Cuts at: 0 1156 1764
Size: 1156 608
PspXI vC'TCGA_Gb
Cuts at: 0 748 1764
Size: 748 1016
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 361 393 1764
Size: 361 32 1371
Fragments arranged by size:
1371 361 32
PstI C_TGCA'G
Cuts at: 0 1337 1764
Size: 1337 427
PvuII CAG'CTG
Cuts at: 0 84 477 1254 1764
Size: 84 393 777 510
Fragments arranged by size:
777 510 393 84
SacII CC_GC'GG
Cuts at: 0 445 1764
Size: 445 1319
SalI G'TCGA_C
Cuts at: 0 1132 1764
Size: 1132 632
SapI GCTCTTCn'nnn_
Cuts at: 0 286 1493 1764
Size: 286 1207 271
Fragments arranged by size:
1207 286 271
ScaI AGT'ACT
Cuts at: 0 1237 1764
Size: 1237 527
SfcI C'TryA_G
Cuts at: 0 173 308 648 1333 1764
Size: 173 135 340 685 431
Fragments arranged by size:
685 431 340 173 135
SmlI C'TyrA_G
Cuts at: 0 10 748 1764
Size: 10 738 1016
Fragments arranged by size:
1016 738 10
StuI AGG'CCT
Cuts at: 0 8 1764
Size: 8 1756
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1135 1179 1297 1764
Size: 1135 44 118 467
Fragments arranged by size:
1135 467 118 44
TatI w'GTAC_w
Cuts at: 0 1235 1764
Size: 1235 529
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1035 1067 1764
Size: 1035 32 697
Fragments arranged by size:
1035 697 32
XhoI C'TCGA_G
Cuts at: 0 748 1764
Size: 748 1016
XmnI GAAnn'nnTTC
Cuts at: 0 994 1764
Size: 994 770
ZraI GAC'GTC
Cuts at: 0 1101 1764
Size: 1101 663
Enzymes that do cut:
AarI AatII AccI AclI AcuI AflIII AloI AlwNI
AvaI BanI BbsI BciVI BglI BlpI Bme1580I BmgBI
BmrI BplI BpmI BpuEI BsaAI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsmI Bsp1286I BspEI BspMI
BsrFI BstXI BtgI ClaI DraI DrdI EaeI EarI
EciI Eco57MI EcoNI EcoO109I FspI HaeII Hin4I HincII
HindIII HpaI MluI MmeI MscI MslI MspA1I NaeI
NgoMIV PasI PflMI PmlI PpiI PpuMI PshAI PspXI
PsrI PstI PvuII SacII SalI SapI ScaI SfcI
SmlI StuI TaqII TatI TstI XhoI XmnI ZraI
Enzymes that do not cut:
Acc65I AfeI AflII AgeI AhdI AleI AlfI ApaI
ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI
BanII BbeI BbvCI BcgI BclI BfrBI BglII BmtI
Bpu10I BsaI BsaBI BsiHKAI BsiWI BsmBI BspHI BsrBI
BsrDI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BstZ17I Bsu36I BtgZI BtsI CspCI DraIII EagI EcoICRI
EcoRI EcoRV FalI FseI FspAI KasI KpnI MfeI
NarI NcoI NdeI NheI NotI NruI NsiI NspI
PacI PciI PfoI PmeI PsiI PspOMI PvuI RsrII
SacI SanDI SbfI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI StyI SwaI
Tth111I XbaI XcmI XmaI