Restriction map for
pF1KB5344
(Linear) (Six-base) MAPSORT of: KIBB5344.seq Check: 8585 from: 1 to: 696
KIBB5344.orf
With 182 enzymes: SgfI *
March 3, 2008 10:57 ..
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 473 494 696
Size: 473 21 202
Fragments arranged by size:
473 202 21
ApaI G_GGCC'C
Cuts at: 0 18 696
Size: 18 678
BanI G'GyrC_C
Cuts at: 0 432 696
Size: 432 264
BanII G_rGCy'C
Cuts at: 0 18 595 696
Size: 18 577 101
Fragments arranged by size:
577 101 18
BbeI G_GCGC'C
Cuts at: 0 436 696
Size: 436 260
BbsI GAAGACnn'nnnn_
Cuts at: 0 82 696
Size: 82 614
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 238 272 696
Size: 238 34 424
Fragments arranged by size:
424 238 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 192 696
Size: 192 504
BlpI GC'TnA_GC
Cuts at: 0 501 696
Size: 501 195
Bme1580I G_kGCm'C
Cuts at: 0 18 131 696
Size: 18 113 565
Fragments arranged by size:
565 113 18
BmgBI CAC'GTC
Cuts at: 0 63 696
Size: 63 633
BmrI ACTGGGnnnn_n'
Cuts at: 0 626 696
Size: 626 70
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 295 696
Size: 295 401
BsaI GGTCTCn'nnnn_
Cuts at: 0 112 696
Size: 112 584
BsaBI GATnn'nnATC
Cuts at: 0 110 696
Size: 110 586
BsaHI Gr'CG_yC
Cuts at: 0 433 696
Size: 433 263
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 605 635 696
Size: 605 30 61
Fragments arranged by size:
605 61 30
BseYI C'CCAG_C
Cuts at: 0 20 346 474 696
Size: 20 326 128 222
Fragments arranged by size:
326 222 128 20
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 85 500 696
Size: 85 415 196
Fragments arranged by size:
415 196 85
BsmBI CGTCTCn'nnnn_
Cuts at: 0 3 696
Size: 3 693
Bsp1286I G_dGCh'C
Cuts at: 0 18 131 595 696
Size: 18 113 464 101
Fragments arranged by size:
464 113 101 18
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 392 696
Size: 392 304
BstBI TT'CG_AA
Cuts at: 0 140 696
Size: 140 556
BstYI r'GATC_y
Cuts at: 0 208 696
Size: 208 488
Bsu36I CC'TnA_GG
Cuts at: 0 204 696
Size: 204 492
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 97 696
Size: 97 599
BtsI GCAGTG_nn'
Cuts at: 0 73 577 696
Size: 73 504 119
Fragments arranged by size:
504 119 73
EaeI y'GGCC_r
Cuts at: 0 32 696
Size: 32 664
EarI CTCTTCn'nnn_
Cuts at: 0 583 657 696
Size: 583 74 39
Fragments arranged by size:
583 74 39
EcoO109I rG'GnC_Cy
Cuts at: 0 15 696
Size: 15 681
HaeII r_GCGC'y
Cuts at: 0 436 696
Size: 436 260
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 29 61 696
Size: 29 32 635
Fragments arranged by size:
635 32 29
KasI G'GCGC_C
Cuts at: 0 432 696
Size: 432 264
MscI TGG'CCA
Cuts at: 0 34 696
Size: 34 662
MspA1I CmG'CkG
Cuts at: 0 474 696
Size: 474 222
NarI GG'CG_CC
Cuts at: 0 433 696
Size: 433 263
PspOMI G'GGCC_C
Cuts at: 0 14 696
Size: 14 682
PvuII CAG'CTG
Cuts at: 0 474 696
Size: 474 222
SapI GCTCTTCn'nnn_
Cuts at: 0 583 696
Size: 583 113
ScaI AGT'ACT
Cuts at: 0 402 696
Size: 402 294
SfcI C'TryA_G
Cuts at: 0 243 696
Size: 243 453
SfoI GGC'GCC
Cuts at: 0 434 696
Size: 434 262
SmlI C'TyrA_G
Cuts at: 0 310 696
Size: 310 386
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 466 696
Size: 466 230
TatI w'GTAC_w
Cuts at: 0 400 696
Size: 400 296
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 52 84 696
Size: 52 32 612
Fragments arranged by size:
612 52 32
Enzymes that do cut:
AlfI ApaI BanI BanII BbeI BbsI BcgI BciVI
BlpI Bme1580I BmgBI BmrI BpuEI BsaI BsaBI BsaHI
BsaXI BseYI BsgI BsmBI Bsp1286I BspMI BstBI BstYI
Bsu36I BtgZI BtsI EaeI EarI EcoO109I HaeII Hin4I
KasI MscI MspA1I NarI PspOMI PvuII SapI ScaI
SfcI SfoI SmlI TaqII TatI TstI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AloI AlwNI ApaLI ApoI
AscI AseI AsiSI AvaI AvrII BaeI BamHI BbvCI
BclI BfrBI BglI BglII BmtI BplI BpmI Bpu10I
BsaAI BsaWI BseRI BsiEI BsiHKAI BsiWI BsmI BspEI
BspHI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstEII BstXI BstZ17I BtgI ClaI CspCI DraI DraIII
DrdI EagI EciI EcoICRI Eco57MI EcoNI EcoRI EcoRV
FalI FseI FspI FspAI HincII HindIII HpaI KpnI
MfeI MluI MmeI MslI NaeI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI PacI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspXI PsrI PstI PvuI RsrII SacI SacII SalI
SanDI SbfI SexAI SfiI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI StuI StyI SwaI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI