Restriction map for
pF1KB5348
(Linear) (Six-base) MAPSORT of: KIBB5348.seq Check: 9080 from: 1 to: 1170
KIBB5348.orf
With 182 enzymes: SgfI *
March 3, 2008 10:57 ..
AccI GT'mk_AC
Cuts at: 0 276 1170
Size: 276 894
Acc65I G'GTAC_C
Cuts at: 0 1064 1170
Size: 1064 106
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 485 1170
Size: 485 685
ApaI G_GGCC'C
Cuts at: 0 899 1170
Size: 899 271
ApoI r'AATT_y
Cuts at: 0 408 1170
Size: 408 762
BanI G'GyrC_C
Cuts at: 0 1064 1170
Size: 1064 106
BanII G_rGCy'C
Cuts at: 0 543 899 1170
Size: 543 356 271
Fragments arranged by size:
543 356 271
BglII A'GATC_T
Cuts at: 0 490 1170
Size: 490 680
Bme1580I G_kGCm'C
Cuts at: 0 899 1170
Size: 899 271
BmrI ACTGGGnnnn_n'
Cuts at: 0 436 1170
Size: 436 734
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1148 1170
Size: 1148 22
Bpu10I CC'TnA_GC
Cuts at: 0 185 1170
Size: 185 985
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 966 990 1170
Size: 966 24 180
Fragments arranged by size:
966 180 24
BsaAI yAC'GTr
Cuts at: 0 553 754 874 949 1078 1170
Size: 553 201 120 75 129 92
Fragments arranged by size:
553 201 129 120 92 75
BsaBI GATnn'nnATC
Cuts at: 0 761 1170
Size: 761 409
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 353 389 533 1170
Size: 353 36 144 637
Fragments arranged by size:
637 353 144 36
BseYI C'CCAG_C
Cuts at: 0 174 1170
Size: 174 996
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 479 1170
Size: 479 691
BsiHKAI G_wGCw'C
Cuts at: 0 543 882 1110 1170
Size: 543 339 228 60
Fragments arranged by size:
543 339 228 60
Bsp1286I G_dGCh'C
Cuts at: 0 543 882 899 1110 1170
Size: 543 339 17 211 60
Fragments arranged by size:
543 339 211 60 17
BsrBI CCG'CTC
Cuts at: 0 594 1170
Size: 594 576
BssSI C'ACGA_G
Cuts at: 0 371 683 713 890 1170
Size: 371 312 30 177 280
Fragments arranged by size:
371 312 280 177 30
BstXI CCAn_nnnn'nTGG
Cuts at: 0 240 1170
Size: 240 930
BstYI r'GATC_y
Cuts at: 0 490 1170
Size: 490 680
BtgI C'CryG_G
Cuts at: 0 1101 1170
Size: 1101 69
BtsI GCAGTG_nn'
Cuts at: 0 528 1170
Size: 528 642
DraIII CAC_nnn'GTG
Cuts at: 0 877 1170
Size: 877 293
EarI CTCTTCn'nnn_
Cuts at: 0 34 330 531 552 1170
Size: 34 296 201 21 618
Fragments arranged by size:
618 296 201 34 21
EcoICRI GAG'CTC
Cuts at: 0 541 1170
Size: 541 629
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 485 1148 1170
Size: 485 663 22
Fragments arranged by size:
663 485 22
EcoNI CCTnn'n_nnAGG
Cuts at: 0 317 371 1170
Size: 317 54 799
Fragments arranged by size:
799 317 54
EcoO109I rG'GnC_Cy
Cuts at: 0 310 361 475 895 896 1170
Size: 310 51 114 420 1 274
Fragments arranged by size:
420 310 274 114 51 1
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 751 783 964 988 996 1020 1170
Size: 751 32 181 24 8 24 150
Fragments arranged by size:
751 181 150 32 24 24 8
KpnI G_GTAC'C
Cuts at: 0 1068 1170
Size: 1068 102
MslI CAynn'nnrTG
Cuts at: 0 238 386 827 1170
Size: 238 148 441 343
Fragments arranged by size:
441 343 238 148
MspA1I CmG'CkG
Cuts at: 0 1103 1170
Size: 1103 67
NdeI CA'TA_TG
Cuts at: 0 852 1170
Size: 852 318
PflMI CCAn_nnn'nTGG
Cuts at: 0 469 910 1170
Size: 469 441 260
Fragments arranged by size:
469 441 260
PmlI CAC'GTG
Cuts at: 0 553 754 949 1078 1170
Size: 553 201 195 129 92
Fragments arranged by size:
553 201 195 129 92
PpuMI rG'GwC_Cy
Cuts at: 0 310 361 475 1170
Size: 310 51 114 695
Fragments arranged by size:
695 310 114 51
PspOMI G'GGCC_C
Cuts at: 0 895 1170
Size: 895 275
SacI G_AGCT'C
Cuts at: 0 543 1170
Size: 543 627
SacII CC_GC'GG
Cuts at: 0 1104 1170
Size: 1104 66
SapI GCTCTTCn'nnn_
Cuts at: 0 34 531 1170
Size: 34 497 639
Fragments arranged by size:
639 497 34
ScaI AGT'ACT
Cuts at: 0 110 1170
Size: 110 1060
SfcI C'TryA_G
Cuts at: 0 840 1052 1086 1170
Size: 840 212 34 84
Fragments arranged by size:
840 212 84 34
SmlI C'TyrA_G
Cuts at: 0 981 1005 1170
Size: 981 24 165
Fragments arranged by size:
981 165 24
SpeI A'CTAG_T
Cuts at: 0 507 1170
Size: 507 663
TatI w'GTAC_w
Cuts at: 0 108 1170
Size: 108 1062
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 86 118 1170
Size: 86 32 1052
Fragments arranged by size:
1052 86 32
Enzymes that do cut:
AccI Acc65I AcuI ApaI ApoI BanI BanII BglII
Bme1580I BmrI BpmI Bpu10I BpuEI BsaAI BsaBI BseRI
BseYI BsgI BsiHKAI Bsp1286I BsrBI BssSI BstXI BstYI
BtgI BtsI DraIII EarI EcoICRI Eco57MI EcoNI EcoO109I
Hin4I KpnI MslI MspA1I NdeI PflMI PmlI PpuMI
PspOMI SacI SacII SapI ScaI SfcI SmlI SpeI
TatI TstI
Enzymes that do not cut:
AarI AatII AclI AfeI AflII AflIII AgeI AhdI
AleI AlfI AloI AlwNI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BbeI BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BlpI BmgBI BmtI BplI
BsaI BsaHI BsaWI BsaXI BsiEI BsiWI BsmI BsmBI
BspEI BspHI BspMI BsrDI BsrFI BsrGI BssHII BstAPI
BstBI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraI
DrdI EaeI EagI EciI EcoRI EcoRV FalI FseI
FspI FspAI HaeII HincII HindIII HpaI KasI MfeI
MluI MmeI MscI NaeI NarI NcoI NgoMIV NheI
NotI NruI NsiI NspI PacI PasI PciI PfoI
PmeI PpiI PshAI PsiI PspXI PsrI PstI PvuI
PvuII RsrII SalI SanDI SbfI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SphI SrfI SspI StuI
StyI SwaI TaqII Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI