Restriction map for
pF1KB5356
(Linear) (Six-base) MAPSORT of: KIBB5356.seq Check: 3692 from: 1 to: 1344
KIBB5356.orf
With 182 enzymes: SgfI *
April 24, 2009 13:04 ..
AatII G_ACGT'C
Cuts at: 0 55 1344
Size: 55 1289
ApaI G_GGCC'C
Cuts at: 0 112 670 1344
Size: 112 558 674
Fragments arranged by size:
674 558 112
AvaI C'yCGr_G
Cuts at: 0 205 1090 1344
Size: 205 885 254
Fragments arranged by size:
885 254 205
BanI G'GyrC_C
Cuts at: 0 769 937 1344
Size: 769 168 407
Fragments arranged by size:
769 407 168
BanII G_rGCy'C
Cuts at: 0 76 112 670 860 1271 1344
Size: 76 36 558 190 411 73
Fragments arranged by size:
558 411 190 76 73 36
BbeI G_GCGC'C
Cuts at: 0 773 1344
Size: 773 571
BbsI GAAGACnn'nnnn_
Cuts at: 0 515 980 1344
Size: 515 465 364
Fragments arranged by size:
515 465 364
BclI T'GATC_A
Cuts at: 0 263 434 515 701 1344
Size: 263 171 81 186 643
Fragments arranged by size:
643 263 186 171 81
BglI GCCn_nnn'nGGC
Cuts at: 0 98 1344
Size: 98 1246
Bme1580I G_kGCm'C
Cuts at: 0 112 670 942 1344
Size: 112 558 272 402
Fragments arranged by size:
558 402 272 112
BmrI ACTGGGnnnn_n'
Cuts at: 0 129 1344
Size: 129 1215
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 244 928 1075 1344
Size: 244 684 147 269
Fragments arranged by size:
684 269 244 147
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 559 1344
Size: 559 785
BsaHI Gr'CG_yC
Cuts at: 0 52 770 1344
Size: 52 718 574
Fragments arranged by size:
718 574 52
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 172 184 394 918 1063 1078 1344
Size: 172 12 210 524 145 15 266
Fragments arranged by size:
524 266 210 172 145 15 12
BsiEI CG_ry'CG
Cuts at: 0 68 214 1306 1344
Size: 68 146 1092 38
Fragments arranged by size:
1092 146 68 38
BsiHKAI G_wGCw'C
Cuts at: 0 476 575 1344
Size: 476 99 769
Fragments arranged by size:
769 476 99
BsmI GAATG_Cn'
Cuts at: 0 830 1344
Size: 830 514
Bsp1286I G_dGCh'C
Cuts at: 0 76 112 476 575 670 860 942 1271
Size: 76 36 364 99 95 190 82 329
Cuts at: 1271 1344
Size: 73
Fragments arranged by size:
364 329 190 99 95 82 76 73
36
BspHI T'CATG_A
Cuts at: 0 431 698 1344
Size: 431 267 646
Fragments arranged by size:
646 431 267
BsrBI CCG'CTC
Cuts at: 0 48 1344
Size: 48 1296
BssSI C'ACGA_G
Cuts at: 0 790 1344
Size: 790 554
BstAPI GCAn_nnn'nTGC
Cuts at: 0 650 1344
Size: 650 694
BstXI CCAn_nnnn'nTGG
Cuts at: 0 465 1344
Size: 465 879
BtgI C'CryG_G
Cuts at: 0 504 593 890 1136 1286 1344
Size: 504 89 297 246 150 58
Fragments arranged by size:
504 297 246 150 89 58
BtsI GCAGTG_nn'
Cuts at: 0 644 1344
Size: 644 700
DraIII CAC_nnn'GTG
Cuts at: 0 290 1344
Size: 290 1054
DrdI GACnn_nn'nnGTC
Cuts at: 0 187 1344
Size: 187 1157
EaeI y'GGCC_r
Cuts at: 0 65 211 590 1178 1303 1344
Size: 65 146 379 588 125 41
Fragments arranged by size:
588 379 146 125 65 41
EagI C'GGCC_G
Cuts at: 0 65 211 1303 1344
Size: 65 146 1092 41
Fragments arranged by size:
1092 146 65 41
EarI CTCTTCn'nnn_
Cuts at: 0 224 550 1055 1344
Size: 224 326 505 289
Fragments arranged by size:
505 326 289 224
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 244 928 1075 1344
Size: 244 684 147 269
Fragments arranged by size:
684 269 244 147
EcoO109I rG'GnC_Cy
Cuts at: 0 108 174 666 667 814 1344
Size: 108 66 492 1 147 530
Fragments arranged by size:
530 492 147 108 66 1
HaeII r_GCGC'y
Cuts at: 0 402 773 1237 1344
Size: 402 371 464 107
Fragments arranged by size:
464 402 371 107
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 37 69 341 370 373 402 913 945
Size: 37 32 272 29 3 29 511 32
Cuts at: 945 1344
Size: 399
Fragments arranged by size:
511 399 272 37 32 32 29 29
3
HindIII A'AGCT_T
Cuts at: 0 1081 1344
Size: 1081 263
KasI G'GCGC_C
Cuts at: 0 769 1344
Size: 769 575
MscI TGG'CCA
Cuts at: 0 592 1180 1344
Size: 592 588 164
Fragments arranged by size:
592 588 164
MslI CAynn'nnrTG
Cuts at: 0 10 298 778 1344
Size: 10 288 480 566
Fragments arranged by size:
566 480 288 10
MspA1I CmG'CkG
Cuts at: 0 488 1145 1344
Size: 488 657 199
Fragments arranged by size:
657 488 199
NarI GG'CG_CC
Cuts at: 0 770 1344
Size: 770 574
NcoI C'CATG_G
Cuts at: 0 504 593 890 1344
Size: 504 89 297 454
Fragments arranged by size:
504 454 297 89
NotI GC'GGCC_GC
Cuts at: 0 211 1344
Size: 211 1133
NspI r_CATG'y
Cuts at: 0 262 1344
Size: 262 1082
PasI CC'CwG_GG
Cuts at: 0 1042 1344
Size: 1042 302
PflMI CCAn_nnn'nTGG
Cuts at: 0 118 896 1344
Size: 118 778 448
Fragments arranged by size:
778 448 118
PpuMI rG'GwC_Cy
Cuts at: 0 174 1344
Size: 174 1170
PspOMI G'GGCC_C
Cuts at: 0 108 666 1344
Size: 108 558 678
Fragments arranged by size:
678 558 108
PstI C_TGCA'G
Cuts at: 0 683 1344
Size: 683 661
SapI GCTCTTCn'nnn_
Cuts at: 0 224 1344
Size: 224 1120
SexAI A'CCwGG_T
Cuts at: 0 1295 1344
Size: 1295 49
SfcI C'TryA_G
Cuts at: 0 192 679 1107 1344
Size: 192 487 428 237
Fragments arranged by size:
487 428 237 192
SfoI GGC'GCC
Cuts at: 0 771 1344
Size: 771 573
SmaI CCC'GGG
Cuts at: 0 207 1344
Size: 207 1137
SmlI C'TyrA_G
Cuts at: 0 574 1344
Size: 574 770
StuI AGG'CCT
Cuts at: 0 221 688 1344
Size: 221 467 656
Fragments arranged by size:
656 467 221
StyI C'CwwG_G
Cuts at: 0 504 561 593 890 1344
Size: 504 57 32 297 454
Fragments arranged by size:
504 454 297 57 32
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 22 54 1344
Size: 22 32 1290
Fragments arranged by size:
1290 32 22
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 465 1293 1344
Size: 465 828 51
Fragments arranged by size:
828 465 51
XmaI C'CCGG_G
Cuts at: 0 205 1344
Size: 205 1139
ZraI GAC'GTC
Cuts at: 0 53 1344
Size: 53 1291
Enzymes that do cut:
AatII ApaI AvaI BanI BanII BbeI BbsI BclI
BglI Bme1580I BmrI BpmI BpuEI BsaHI BseRI BsiEI
BsiHKAI BsmI Bsp1286I BspHI BsrBI BssSI BstAPI BstXI
BtgI BtsI DraIII DrdI EaeI EagI EarI Eco57MI
EcoO109I HaeII Hin4I HindIII KasI MscI MslI MspA1I
NarI NcoI NotI NspI PasI PflMI PpuMI PspOMI
PstI SapI SexAI SfcI SfoI SmaI SmlI StuI
StyI TstI XcmI XmaI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AscI AseI AsiSI AvrII BaeI BamHI BbvCI BcgI
BciVI BfrBI BglII BlpI BmgBI BmtI BplI Bpu10I
BsaI BsaAI BsaBI BsaWI BsaXI BseYI BsgI BsiWI
BsmBI BspEI BspMI BsrDI BsrFI BsrGI BssHII BstBI
BstEII BstYI BstZ17I Bsu36I BtgZI ClaI CspCI DraI
EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI
FspAI HincII HpaI KpnI MfeI MluI MmeI NaeI
NdeI NgoMIV NheI NruI NsiI PacI PciI PfoI
PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI
PvuII RsrII SacI SacII SalI SanDI SbfI ScaI
SfiI SgfI SgrAI SnaBI SpeI SphI SrfI SspI
SwaI TaqII TatI Tth111I XbaI XhoI XmnI