(Linear) (Six-base) MAPSORT of: KIBB5359.seq  Check: 8427  from: 1  to: 549



KIBB5359.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:57  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     101     166     209     549 
   Size:        101      65      43     340

  Fragments arranged by size: 

                 340     101      65      43
 
AflIII A'CryG_T

Cuts at:      0     114     549 
   Size:        114     435
 
AlwNI CAG_nnn'CTG

Cuts at:      0      79     169     549 
   Size:         79      90     380

  Fragments arranged by size: 

                 380      90      79
 
ApoI r'AATT_y

Cuts at:      0      85     195     549 
   Size:         85     110     354

  Fragments arranged by size: 

                 354     110      85
 
BanI G'GyrC_C

Cuts at:      0     382     549 
   Size:        382     167
 
BmgBI CAC'GTC

Cuts at:      0     115     549 
   Size:        115     434
 
BsmI GAATG_Cn'

Cuts at:      0     522     549 
   Size:        522      27
 
BspHI T'CATG_A

Cuts at:      0     416     549 
   Size:        416     133
 
BsrDI GCAATG_nn'

Cuts at:      0      19     549 
   Size:         19     530
 
BtsI GCAGTG_nn'

Cuts at:      0     402     549 
   Size:        402     147
 
EarI CTCTTCn'nnn_

Cuts at:      0      62     500     549 
   Size:         62     438      49

  Fragments arranged by size: 

                 438      62      49
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     101     166     209     549 
   Size:        101      65      43     340

  Fragments arranged by size: 

                 340     101      65      43
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     244     293     549 
   Size:        244      49     256

  Fragments arranged by size: 

                 256     244      49
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     277     292     309     324     549 
   Size:        277      15      17      15     225

  Fragments arranged by size: 

                 277     225      17      15      15
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      32     549 
   Size:         32     517
 
ScaI AGT'ACT

Cuts at:      0     104     549 
   Size:        104     445
 
SspI AAT'ATT

Cuts at:      0     139     549 
   Size:        139     410
 
TatI w'GTAC_w

Cuts at:      0     102     549 
   Size:        102     447
 
XmnI GAAnn'nnTTC

Cuts at:      0     224     549 
   Size:        224     325
 

 Enzymes that do cut:

     AcuI   AflIII    AlwNI     ApoI     BanI    BmgBI     BsmI    BspHI
    BsrDI     BtsI     EarI  Eco57MI     FalI    Hin4I     MmeI     ScaI
     SspI     TatI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI    BanII     BbeI     BbsI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
 Bme1580I     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI
    BsiEI  BsiHKAI    BsiWI    BsmBI Bsp1286I    BspEI    BspMI    BsrBI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI
    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI    CspCI     DraI
   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI    EcoNI EcoO109I
    EcoRI    EcoRV     FseI     FspI    FspAI    HaeII   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MscI     MslI   MspA1I
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     StuI     StyI
     SwaI    TaqII     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI
     ZraI