Restriction map for
pF1KB5360
(Linear) (Six-base) MAPSORT of: KIBB5360.seq Check: 9371 from: 1 to: 1104
KIBB5360.orf
With 182 enzymes: SgfI *
October 7, 2009 14:16 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 785 1104
Size: 785 319
AlwNI CAG_nnn'CTG
Cuts at: 0 896 1104
Size: 896 208
ApoI r'AATT_y
Cuts at: 0 607 1027 1104
Size: 607 420 77
Fragments arranged by size:
607 420 77
BanI G'GyrC_C
Cuts at: 0 856 1104
Size: 856 248
BbvCI CC'TCA_GC
Cuts at: 0 343 1104
Size: 343 761
BciVI GTATCCnnnnn_n'
Cuts at: 0 462 1104
Size: 462 642
BmgBI CAC'GTC
Cuts at: 0 531 1104
Size: 531 573
BmrI ACTGGGnnnn_n'
Cuts at: 0 266 446 1104
Size: 266 180 658
Fragments arranged by size:
658 266 180
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 300 1104
Size: 300 804
Bpu10I CC'TnA_GC
Cuts at: 0 343 1104
Size: 343 761
BsaBI GATnn'nnATC
Cuts at: 0 364 1104
Size: 364 740
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 93 123 789 819 1104
Size: 93 30 666 30 285
Fragments arranged by size:
666 285 93 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 797 1104
Size: 797 307
BseYI C'CCAG_C
Cuts at: 0 203 242 443 1104
Size: 203 39 201 661
Fragments arranged by size:
661 203 201 39
BsiEI CG_ry'CG
Cuts at: 0 478 1104
Size: 478 626
BstXI CCAn_nnnn'nTGG
Cuts at: 0 549 1104
Size: 549 555
BstYI r'GATC_y
Cuts at: 0 874 1104
Size: 874 230
Bsu36I CC'TnA_GG
Cuts at: 0 21 809 1104
Size: 21 788 295
Fragments arranged by size:
788 295 21
BtgI C'CryG_G
Cuts at: 0 542 1104
Size: 542 562
BtsI GCAGTG_nn'
Cuts at: 0 63 1104
Size: 63 1041
ClaI AT'CG_AT
Cuts at: 0 780 1104
Size: 780 324
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 134 169 1104
Size: 134 35 935
Fragments arranged by size:
935 134 35
DraI TTT'AAA
Cuts at: 0 458 663 771 1104
Size: 458 205 108 333
Fragments arranged by size:
458 333 205 108
EarI CTCTTCn'nnn_
Cuts at: 0 1073 1104
Size: 1073 31
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 300 785 1104
Size: 300 485 319
Fragments arranged by size:
485 319 300
EcoO109I rG'GnC_Cy
Cuts at: 0 339 570 1104
Size: 339 231 534
Fragments arranged by size:
534 339 231
EcoRI G'AATT_C
Cuts at: 0 607 1104
Size: 607 497
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 396 428 460 492 567 599 609 641
Size: 396 32 32 32 75 32 10 32
Cuts at: 641 1104
Size: 463
Fragments arranged by size:
463 396 75 32 32 32 32 32
10
MslI CAynn'nnrTG
Cuts at: 0 547 647 1104
Size: 547 100 457
Fragments arranged by size:
547 457 100
MspA1I CmG'CkG
Cuts at: 0 425 1104
Size: 425 679
NcoI C'CATG_G
Cuts at: 0 542 1104
Size: 542 562
NspI r_CATG'y
Cuts at: 0 51 1104
Size: 51 1053
PasI CC'CwG_GG
Cuts at: 0 696 1104
Size: 696 408
PflMI CCAn_nnn'nTGG
Cuts at: 0 335 401 1104
Size: 335 66 703
Fragments arranged by size:
703 335 66
PpuMI rG'GwC_Cy
Cuts at: 0 339 1104
Size: 339 765
PsiI TTA'TAA
Cuts at: 0 1034 1104
Size: 1034 70
PstI C_TGCA'G
Cuts at: 0 564 1104
Size: 564 540
PvuI CG_AT'CG
Cuts at: 0 478 1104
Size: 478 626
PvuII CAG'CTG
Cuts at: 0 425 1104
Size: 425 679
SapI GCTCTTCn'nnn_
Cuts at: 0 1073 1104
Size: 1073 31
SfcI C'TryA_G
Cuts at: 0 65 420 560 1104
Size: 65 355 140 544
Fragments arranged by size:
544 355 140 65
StyI C'CwwG_G
Cuts at: 0 542 1104
Size: 542 562
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 789 821 1104
Size: 789 32 283
Fragments arranged by size:
789 283 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 549 850 1104
Size: 549 301 254
Fragments arranged by size:
549 301 254
Enzymes that do cut:
AcuI AlwNI ApoI BanI BbvCI BciVI BmgBI BmrI
BpmI Bpu10I BsaBI BsaXI BseRI BseYI BsiEI BstXI
BstYI Bsu36I BtgI BtsI ClaI CspCI DraI EarI
Eco57MI EcoO109I EcoRI Hin4I MslI MspA1I NcoI NspI
PasI PflMI PpuMI PsiI PstI PvuI PvuII SapI
SfcI StyI TstI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaI ApaLI AscI
AseI AsiSI AvaI AvrII BaeI BamHI BanII BbeI
BbsI BcgI BclI BfrBI BglI BglII BlpI Bme1580I
BmtI BplI BpuEI BsaI BsaAI BsaHI BsaWI BsgI
BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstZ17I BtgZI DraIII DrdI EaeI EagI EciI
EcoICRI EcoNI EcoRV FalI FseI FspI FspAI HaeII
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI NaeI NarI NdeI NgoMIV NheI NotI NruI
NsiI PacI PciI PfoI PmeI PmlI PpiI PshAI
PspOMI PspXI PsrI RsrII SacI SacII SalI SanDI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI
TaqII TatI Tth111I XbaI XhoI XmaI XmnI ZraI