(Linear) (Six-base) MAPSORT of: KIBB5363.seq  Check: 6505  from: 1  to: 462



KIBB5363.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:27  ..

AccI GT'mk_AC

Cuts at:      0     143     462 
   Size:        143     319
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     209     462 
   Size:        209     253
 
Bme1580I G_kGCm'C

Cuts at:      0     409     462 
   Size:        409      53
 
BmtI G_CTAG'C

Cuts at:      0      74     175     462 
   Size:         74     101     287

  Fragments arranged by size: 

                 287     101      74
 
BsaAI yAC'GTr

Cuts at:      0     404     462 
   Size:        404      58
 
BsiEI CG_ry'CG

Cuts at:      0      66     462 
   Size:         66     396
 
Bsp1286I G_dGCh'C

Cuts at:      0     409     462 
   Size:        409      53
 
BssSI C'ACGA_G

Cuts at:      0      49     462 
   Size:         49     413
 
BstYI r'GATC_y

Cuts at:      0     284     394     462 
   Size:        284     110      68

  Fragments arranged by size: 

                 284     110      68
 
BtsI GCAGTG_nn'

Cuts at:      0     436     462 
   Size:        436      26
 
EarI CTCTTCn'nnn_

Cuts at:      0     185     258     462 
   Size:        185      73     204

  Fragments arranged by size: 

                 204     185      73
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     209     462 
   Size:        209     253
 
EcoO109I rG'GnC_Cy

Cuts at:      0     327     462 
   Size:        327     135
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      22      43      54      75     462 
   Size:         22      21      11      21     387

  Fragments arranged by size: 

                 387      22      21      21      11
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      86     462 
   Size:         86     376
 
MspA1I CmG'CkG

Cuts at:      0     183     462 
   Size:        183     279
 
NheI G'CTAG_C

Cuts at:      0      70     171     462 
   Size:         70     101     291

  Fragments arranged by size: 

                 291     101      70
 
NspI r_CATG'y

Cuts at:      0     354     462 
   Size:        354     108
 
PfoI T'CCnGG_A

Cuts at:      0     287     462 
   Size:        287     175
 
PmlI CAC'GTG

Cuts at:      0     404     462 
   Size:        404      58
 
PpuMI rG'GwC_Cy

Cuts at:      0     327     462 
   Size:        327     135
 
PvuII CAG'CTG

Cuts at:      0     183     462 
   Size:        183     279
 
SanDI GG'GwC_CC

Cuts at:      0     327     462 
   Size:        327     135
 
SphI G_CATG'C

Cuts at:      0     354     462 
   Size:        354     108
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     164     462 
   Size:        164     298
 
XmnI GAAnn'nnTTC

Cuts at:      0     128     462 
   Size:        128     334
 

 Enzymes that do cut:

     AccI     AcuI Bme1580I     BmtI    BsaAI    BsiEI Bsp1286I    BssSI
    BstYI     BtsI     EarI  Eco57MI EcoO109I    Hin4I     MmeI   MspA1I
     NheI     NspI     PfoI     PmlI    PpuMI    PvuII    SanDI     SphI
    TaqII     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI
     BglI    BglII     BlpI    BmgBI     BmrI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
     BsgI  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI
    BsrBI    BsrDI    BsrFI    BsrGI   BssHII   BstAPI    BstBI   BstEII
    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI    CspCI     DraI
   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI    EcoNI    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI    HaeII   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MscI     MslI     NaeI
     NarI     NcoI     NdeI   NgoMIV     NotI     NruI     NsiI     PacI
     PasI     PciI    PflMI     PmeI     PpiI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PstI     PvuI    RsrII     SacI    SacII     SalI
     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SrfI     SspI     StuI
     StyI     SwaI     TatI     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI     ZraI