Restriction map for
pF1KB5366
(Linear) (Six-base) MAPSORT of: KIBB5366.seq Check: 5171 from: 1 to: 633
KIBB5366.orf
With 182 enzymes: SgfI *
October 7, 2009 14:16 ..
Acc65I G'GTAC_C
Cuts at: 0 194 633
Size: 194 439
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 210 370 443 633
Size: 210 160 73 190
Fragments arranged by size:
210 190 160 73
AgeI A'CCGG_T
Cuts at: 0 456 633
Size: 456 177
AleI CACnn'nnGTG
Cuts at: 0 205 633
Size: 205 428
AlwNI CAG_nnn'CTG
Cuts at: 0 348 633
Size: 348 285
ApoI r'AATT_y
Cuts at: 0 522 633
Size: 522 111
AscI GG'CGCG_CC
Cuts at: 0 127 633
Size: 127 506
AvaI C'yCGr_G
Cuts at: 0 497 633
Size: 497 136
BamHI G'GATC_C
Cuts at: 0 294 633
Size: 294 339
BanI G'GyrC_C
Cuts at: 0 3 194 633
Size: 3 191 439
Fragments arranged by size:
439 191 3
BanII G_rGCy'C
Cuts at: 0 242 633
Size: 242 391
BbeI G_GCGC'C
Cuts at: 0 7 633
Size: 7 626
BglI GCCn_nnn'nGGC
Cuts at: 0 17 633
Size: 17 616
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 378 633
Size: 378 255
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 253 633
Size: 253 380
Bpu10I CC'TnA_GC
Cuts at: 0 241 633
Size: 241 392
BsaAI yAC'GTr
Cuts at: 0 71 633
Size: 71 562
BsaHI Gr'CG_yC
Cuts at: 0 4 633
Size: 4 629
BsaWI w'CCGG_w
Cuts at: 0 451 456 470 633
Size: 451 5 14 163
Fragments arranged by size:
451 163 14 5
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 250 315 633
Size: 250 65 318
Fragments arranged by size:
318 250 65
BseYI C'CCAG_C
Cuts at: 0 7 443 633
Size: 7 436 190
Fragments arranged by size:
436 190 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 361 633
Size: 361 272
BsiEI CG_ry'CG
Cuts at: 0 219 633
Size: 219 414
BsiHKAI G_wGCw'C
Cuts at: 0 242 633
Size: 242 391
BsmI GAATG_Cn'
Cuts at: 0 254 633
Size: 254 379
Bsp1286I G_dGCh'C
Cuts at: 0 242 633
Size: 242 391
BspEI T'CCGG_A
Cuts at: 0 470 633
Size: 470 163
BsrBI CCG'CTC
Cuts at: 0 362 633
Size: 362 271
BsrFI r'CCGG_y
Cuts at: 0 84 214 250 456 633
Size: 84 130 36 206 177
Fragments arranged by size:
206 177 130 84 36
BssHII G'CGCG_C
Cuts at: 0 127 210 633
Size: 127 83 423
Fragments arranged by size:
423 127 83
BstYI r'GATC_y
Cuts at: 0 95 294 633
Size: 95 199 339
Fragments arranged by size:
339 199 95
BtgI C'CryG_G
Cuts at: 0 185 219 317 633
Size: 185 34 98 316
Fragments arranged by size:
316 185 98 34
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 120 633
Size: 120 513
EaeI y'GGCC_r
Cuts at: 0 65 216 248 437 633
Size: 65 151 32 189 196
Fragments arranged by size:
196 189 151 65 32
EagI C'GGCC_G
Cuts at: 0 216 633
Size: 216 417
EarI CTCTTCn'nnn_
Cuts at: 0 382 509 577 633
Size: 382 127 68 56
Fragments arranged by size:
382 127 68 56
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 321 633
Size: 321 312
EcoICRI GAG'CTC
Cuts at: 0 240 633
Size: 240 393
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 210 253 370 443 633
Size: 210 43 117 73 190
Fragments arranged by size:
210 190 117 73 43
EcoO109I rG'GnC_Cy
Cuts at: 0 157 633
Size: 157 476
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 54 633
Size: 54 579
HaeII r_GCGC'y
Cuts at: 0 7 633
Size: 7 626
KasI G'GCGC_C
Cuts at: 0 3 633
Size: 3 630
KpnI G_GTAC'C
Cuts at: 0 198 633
Size: 198 435
MscI TGG'CCA
Cuts at: 0 67 439 633
Size: 67 372 194
Fragments arranged by size:
372 194 67
MslI CAynn'nnrTG
Cuts at: 0 205 289 633
Size: 205 84 344
Fragments arranged by size:
344 205 84
MspA1I CmG'CkG
Cuts at: 0 57 221 345 447 633
Size: 57 164 124 102 186
Fragments arranged by size:
186 164 124 102 57
NaeI GCC'GGC
Cuts at: 0 86 216 252 633
Size: 86 130 36 381
Fragments arranged by size:
381 130 86 36
NarI GG'CG_CC
Cuts at: 0 4 633
Size: 4 629
NgoMIV G'CCGG_C
Cuts at: 0 84 214 250 633
Size: 84 130 36 383
Fragments arranged by size:
383 130 84 36
PmlI CAC'GTG
Cuts at: 0 71 633
Size: 71 562
PpuMI rG'GwC_Cy
Cuts at: 0 157 633
Size: 157 476
PspXI vC'TCGA_Gb
Cuts at: 0 497 633
Size: 497 136
PstI C_TGCA'G
Cuts at: 0 332 633
Size: 332 301
PvuII CAG'CTG
Cuts at: 0 447 633
Size: 447 186
RsrII CG'GwC_CG
Cuts at: 0 454 633
Size: 454 179
SacI G_AGCT'C
Cuts at: 0 242 633
Size: 242 391
SacII CC_GC'GG
Cuts at: 0 222 633
Size: 222 411
SanDI GG'GwC_CC
Cuts at: 0 157 633
Size: 157 476
SapI GCTCTTCn'nnn_
Cuts at: 0 577 633
Size: 577 56
SbfI CC_TGCA'GG
Cuts at: 0 332 633
Size: 332 301
SfcI C'TryA_G
Cuts at: 0 328 633
Size: 328 305
SfoI GGC'GCC
Cuts at: 0 5 633
Size: 5 628
SmlI C'TyrA_G
Cuts at: 0 497 633
Size: 497 136
StyI C'CwwG_G
Cuts at: 0 122 596 633
Size: 122 474 37
Fragments arranged by size:
474 122 37
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 457 633
Size: 457 176
XhoI C'TCGA_G
Cuts at: 0 497 633
Size: 497 136
XmnI GAAnn'nnTTC
Cuts at: 0 523 633
Size: 523 110
Enzymes that do cut:
Acc65I AcuI AgeI AleI AlwNI ApoI AscI AvaI
BamHI BanI BanII BbeI BglI BplI BpmI Bpu10I
BsaAI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BsrBI BsrFI BssHII BstYI BtgI
BtgZI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
FalI HaeII KasI KpnI MscI MslI MspA1I NaeI
NarI NgoMIV PmlI PpuMI PspXI PstI PvuII RsrII
SacI SacII SanDI SapI SbfI SfcI SfoI SmlI
StyI TaqII XhoI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AflIII AhdI
AlfI AloI ApaI ApaLI AseI AsiSI AvrII BaeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpuEI BsaI BsaBI BsaXI
BsiWI BsmBI BspHI BspMI BsrDI BsrGI BssSI BstAPI
BstBI BstEII BstXI BstZ17I Bsu36I BtsI ClaI CspCI
DraI DraIII DrdI EcoNI EcoRI EcoRV FseI FspI
FspAI Hin4I HincII HindIII HpaI MfeI MluI MmeI
NcoI NdeI NheI NotI NruI NsiI NspI PacI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PspOMI PsrI PvuI SalI ScaI SexAI SfiI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI
SwaI TatI TstI Tth111I XbaI XcmI XmaI ZraI