Restriction map for
pF1KB5369
(Linear) (Six-base) MAPSORT of: KIBB5369.seq Check: 3331 from: 1 to: 1572
KIBB5369.orf
With 182 enzymes: SgfI *
April 24, 2009 13:04 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 43 1415 1572
Size: 43 1372 157
Fragments arranged by size:
1372 157 43
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 112 1572
Size: 112 1460
AflIII A'CryG_T
Cuts at: 0 674 1572
Size: 674 898
AleI CACnn'nnGTG
Cuts at: 0 145 502 1572
Size: 145 357 1070
Fragments arranged by size:
1070 357 145
AlwNI CAG_nnn'CTG
Cuts at: 0 400 957 1044 1572
Size: 400 557 87 528
Fragments arranged by size:
557 528 400 87
ApaI G_GGCC'C
Cuts at: 0 1396 1572
Size: 1396 176
BanI G'GyrC_C
Cuts at: 0 31 334 376 1016 1572
Size: 31 303 42 640 556
Fragments arranged by size:
640 556 303 42 31
BanII G_rGCy'C
Cuts at: 0 1396 1572
Size: 1396 176
BbsI GAAGACnn'nnnn_
Cuts at: 0 1112 1137 1374 1572
Size: 1112 25 237 198
Fragments arranged by size:
1112 237 198 25
BciVI GTATCCnnnnn_n'
Cuts at: 0 1153 1572
Size: 1153 419
BclI T'GATC_A
Cuts at: 0 650 1572
Size: 650 922
BfrBI ATG'CAT
Cuts at: 0 627 1572
Size: 627 945
BglI GCCn_nnn'nGGC
Cuts at: 0 541 1039 1504 1572
Size: 541 498 465 68
Fragments arranged by size:
541 498 465 68
Bme1580I G_kGCm'C
Cuts at: 0 379 733 1019 1343 1396 1572
Size: 379 354 286 324 53 176
Fragments arranged by size:
379 354 324 286 176 53
BmgBI CAC'GTC
Cuts at: 0 648 1143 1374 1572
Size: 648 495 231 198
Fragments arranged by size:
648 495 231 198
BmrI ACTGGGnnnn_n'
Cuts at: 0 465 1572
Size: 465 1107
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 459 1308 1572
Size: 459 849 264
Fragments arranged by size:
849 459 264
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 142 280 1572
Size: 142 138 1292
Fragments arranged by size:
1292 142 138
Bpu10I CC'TnA_GC
Cuts at: 0 1558 1572
Size: 1558 14
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 202 609 1572
Size: 202 407 963
Fragments arranged by size:
963 407 202
BsaI GGTCTCn'nnnn_
Cuts at: 0 83 244 868 1348 1572
Size: 83 161 624 480 224
Fragments arranged by size:
624 480 224 161 83
BsaAI yAC'GTr
Cuts at: 0 1446 1572
Size: 1446 126
BsaHI Gr'CG_yC
Cuts at: 0 1418 1572
Size: 1418 154
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 778 808 1572
Size: 778 30 764
Fragments arranged by size:
778 764 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 262 1089 1572
Size: 262 827 483
Fragments arranged by size:
827 483 262
BseYI C'CCAG_C
Cuts at: 0 469 520 1572
Size: 469 51 1052
Fragments arranged by size:
1052 469 51
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 436 1396 1572
Size: 436 960 176
Fragments arranged by size:
960 436 176
BsiHKAI G_wGCw'C
Cuts at: 0 1494 1572
Size: 1494 78
Bsp1286I G_dGCh'C
Cuts at: 0 379 733 1019 1343 1396 1494 1572
Size: 379 354 286 324 53 98 78
Fragments arranged by size:
379 354 324 286 98 78 53
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 43 1415 1572
Size: 43 1372 157
Fragments arranged by size:
1372 157 43
BsrDI GCAATG_nn'
Cuts at: 0 1254 1572
Size: 1254 318
BsrFI r'CCGG_y
Cuts at: 0 331 1572
Size: 331 1241
BssSI C'ACGA_G
Cuts at: 0 498 732 1351 1423 1572
Size: 498 234 619 72 149
Fragments arranged by size:
619 498 234 149 72
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1044 1410 1572
Size: 1044 366 162
Fragments arranged by size:
1044 366 162
BstEII G'GTnAC_C
Cuts at: 0 438 1572
Size: 438 1134
BstXI CCAn_nnnn'nTGG
Cuts at: 0 241 1572
Size: 241 1331
BstYI r'GATC_y
Cuts at: 0 953 1572
Size: 953 619
Bsu36I CC'TnA_GG
Cuts at: 0 581 1572
Size: 581 991
BtgI C'CryG_G
Cuts at: 0 380 1011 1118 1170 1295 1359 1397 1572
Size: 380 631 107 52 125 64 38 175
Fragments arranged by size:
631 380 175 125 107 64 52 38
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 594 1572
Size: 594 978
BtsI GCAGTG_nn'
Cuts at: 0 1124 1229 1572
Size: 1124 105 343
Fragments arranged by size:
1124 343 105
DraIII CAC_nnn'GTG
Cuts at: 0 846 1572
Size: 846 726
DrdI GACnn_nn'nnGTC
Cuts at: 0 918 1378 1572
Size: 918 460 194
Fragments arranged by size:
918 460 194
EaeI y'GGCC_r
Cuts at: 0 23 663 944 1376 1482 1572
Size: 23 640 281 432 106 90
Fragments arranged by size:
640 432 281 106 90 23
EarI CTCTTCn'nnn_
Cuts at: 0 763 871 1572
Size: 763 108 701
Fragments arranged by size:
763 701 108
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 112 142 280 1572
Size: 112 30 138 1292
Fragments arranged by size:
1292 138 112 30
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1048 1572
Size: 1048 524
EcoO109I rG'GnC_Cy
Cuts at: 0 318 1041 1392 1393 1525 1572
Size: 318 723 351 1 132 47
Fragments arranged by size:
723 351 318 132 47 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 294 1242 1572
Size: 294 948 330
Fragments arranged by size:
948 330 294
HaeII r_GCGC'y
Cuts at: 0 332 527 1572
Size: 332 195 1045
Fragments arranged by size:
1045 332 195
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 544 576 1274 1306 1572
Size: 544 32 698 32 266
Fragments arranged by size:
698 544 266 32 32
HincII GTy'rAC
Cuts at: 0 759 1572
Size: 759 813
MscI TGG'CCA
Cuts at: 0 25 946 1572
Size: 25 921 626
Fragments arranged by size:
921 626 25
MslI CAynn'nnrTG
Cuts at: 0 145 502 646 1572
Size: 145 357 144 926
Fragments arranged by size:
926 357 145 144
NaeI GCC'GGC
Cuts at: 0 333 1572
Size: 333 1239
NcoI C'CATG_G
Cuts at: 0 1295 1397 1572
Size: 1295 102 175
Fragments arranged by size:
1295 175 102
NgoMIV G'CCGG_C
Cuts at: 0 331 1572
Size: 331 1241
NsiI A_TGCA'T
Cuts at: 0 629 1572
Size: 629 943
NspI r_CATG'y
Cuts at: 0 678 885 1572
Size: 678 207 687
Fragments arranged by size:
687 678 207
PasI CC'CwG_GG
Cuts at: 0 241 426 1572
Size: 241 185 1146
Fragments arranged by size:
1146 241 185
PciI A'CATG_T
Cuts at: 0 674 1572
Size: 674 898
PflMI CCAn_nnn'nTGG
Cuts at: 0 83 846 1449 1572
Size: 83 763 603 123
Fragments arranged by size:
763 603 123 83
PmlI CAC'GTG
Cuts at: 0 1446 1572
Size: 1446 126
PpuMI rG'GwC_Cy
Cuts at: 0 318 1572
Size: 318 1254
PshAI GACnn'nnGTC
Cuts at: 0 1120 1572
Size: 1120 452
PspOMI G'GGCC_C
Cuts at: 0 1392 1572
Size: 1392 180
PstI C_TGCA'G
Cuts at: 0 1524 1572
Size: 1524 48
SapI GCTCTTCn'nnn_
Cuts at: 0 763 1572
Size: 763 809
ScaI AGT'ACT
Cuts at: 0 20 82 1572
Size: 20 62 1490
Fragments arranged by size:
1490 62 20
SfcI C'TryA_G
Cuts at: 0 1520 1572
Size: 1520 52
SmlI C'TyrA_G
Cuts at: 0 217 588 1572
Size: 217 371 984
Fragments arranged by size:
984 371 217
StuI AGG'CCT
Cuts at: 0 586 1572
Size: 586 986
StyI C'CwwG_G
Cuts at: 0 461 1295 1388 1397 1572
Size: 461 834 93 9 175
Fragments arranged by size:
834 461 175 93 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 175 439 1572
Size: 175 264 1133
Fragments arranged by size:
1133 264 175
TatI w'GTAC_w
Cuts at: 0 18 80 1572
Size: 18 62 1492
Fragments arranged by size:
1492 62 18
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 238 261 1395 1572
Size: 238 23 1134 177
Fragments arranged by size:
1134 238 177 23
XmnI GAAnn'nnTTC
Cuts at: 0 772 1225 1572
Size: 772 453 347
Fragments arranged by size:
772 453 347
Enzymes that do cut:
AarI AcuI AflIII AleI AlwNI ApaI BanI BanII
BbsI BciVI BclI BfrBI BglI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaXI
BseRI BseYI BsgI BsiHKAI Bsp1286I BspMI BsrDI BsrFI
BssSI BstAPI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI DraIII DrdI EaeI EarI Eco57MI EcoNI EcoO109I
FalI HaeII Hin4I HincII MscI MslI NaeI NcoI
NgoMIV NsiI NspI PasI PciI PflMI PmlI PpuMI
PshAI PspOMI PstI SapI ScaI SfcI SmlI StuI
StyI TaqII TatI XcmI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AlfI AloI ApaLI ApoI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BbeI BbvCI BcgI BglII BlpI
BmtI BsaBI BsaWI BsiEI BsiWI BsmI BsmBI BspEI
BspHI BsrBI BsrGI BssHII BstBI BstZ17I ClaI CspCI
DraI EagI EciI EcoICRI EcoRI EcoRV FseI FspI
FspAI HindIII HpaI KasI KpnI MfeI MluI MmeI
MspA1I NarI NdeI NheI NotI NruI PacI PfoI
PmeI PpiI PsiI PspXI PsrI PvuI PvuII RsrII
SacI SacII SalI SanDI SbfI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
SwaI TstI Tth111I XbaI XhoI XmaI ZraI