Restriction map for
pF1KB5370
(Linear) (Six-base) MAPSORT of: KIBB5370.seq Check: 4826 from: 1 to: 915
KIBB5370.orf
With 182 enzymes: SgfI *
October 7, 2009 14:16 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 803 915
Size: 803 112
AccI GT'mk_AC
Cuts at: 0 347 915
Size: 347 568
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 373 433 626 915
Size: 373 60 193 289
Fragments arranged by size:
373 289 193 60
AlwNI CAG_nnn'CTG
Cuts at: 0 402 915
Size: 402 513
ApaLI G'TGCA_C
Cuts at: 0 361 915
Size: 361 554
ApoI r'AATT_y
Cuts at: 0 370 915
Size: 370 545
AvaI C'yCGr_G
Cuts at: 0 518 915
Size: 518 397
BamHI G'GATC_C
Cuts at: 0 5 915
Size: 5 910
BanII G_rGCy'C
Cuts at: 0 19 79 915
Size: 19 60 836
Fragments arranged by size:
836 60 19
Bme1580I G_kGCm'C
Cuts at: 0 365 915
Size: 365 550
BmrI ACTGGGnnnn_n'
Cuts at: 0 544 612 892 915
Size: 544 68 280 23
Fragments arranged by size:
544 280 68 23
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 788 915
Size: 788 127
BsaBI GATnn'nnATC
Cuts at: 0 314 915
Size: 314 601
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 871 915
Size: 871 44
BsiHKAI G_wGCw'C
Cuts at: 0 365 915
Size: 365 550
Bsp1286I G_dGCh'C
Cuts at: 0 19 79 365 915
Size: 19 60 286 550
Fragments arranged by size:
550 286 60 19
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 149 803 915
Size: 149 654 112
Fragments arranged by size:
654 149 112
BsrBI CCG'CTC
Cuts at: 0 802 915
Size: 802 113
BsrFI r'CCGG_y
Cuts at: 0 101 547 915
Size: 101 446 368
Fragments arranged by size:
446 368 101
BstXI CCAn_nnnn'nTGG
Cuts at: 0 746 915
Size: 746 169
BstYI r'GATC_y
Cuts at: 0 5 330 609 915
Size: 5 325 279 306
Fragments arranged by size:
325 306 279 5
Bsu36I CC'TnA_GG
Cuts at: 0 107 377 915
Size: 107 270 538
Fragments arranged by size:
538 270 107
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 246 281 915
Size: 246 35 634
Fragments arranged by size:
634 246 35
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 373 433 626 788 915
Size: 373 60 193 162 127
Fragments arranged by size:
373 193 162 127 60
EcoNI CCTnn'n_nnAGG
Cuts at: 0 380 915
Size: 380 535
EcoO109I rG'GnC_Cy
Cuts at: 0 95 915
Size: 95 820
EcoRV GAT'ATC
Cuts at: 0 657 915
Size: 657 258
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 637 915
Size: 637 278
MslI CAynn'nnrTG
Cuts at: 0 568 915
Size: 568 347
MspA1I CmG'CkG
Cuts at: 0 579 855 915
Size: 579 276 60
Fragments arranged by size:
579 276 60
NaeI GCC'GGC
Cuts at: 0 103 549 915
Size: 103 446 366
Fragments arranged by size:
446 366 103
NgoMIV G'CCGG_C
Cuts at: 0 101 547 915
Size: 101 446 368
Fragments arranged by size:
446 368 101
PasI CC'CwG_GG
Cuts at: 0 36 915
Size: 36 879
PflMI CCAn_nnn'nTGG
Cuts at: 0 222 915
Size: 222 693
PfoI T'CCnGG_A
Cuts at: 0 119 785 915
Size: 119 666 130
Fragments arranged by size:
666 130 119
PpuMI rG'GwC_Cy
Cuts at: 0 95 915
Size: 95 820
PstI C_TGCA'G
Cuts at: 0 689 915
Size: 689 226
PvuII CAG'CTG
Cuts at: 0 579 855 915
Size: 579 276 60
Fragments arranged by size:
579 276 60
SfcI C'TryA_G
Cuts at: 0 574 685 753 915
Size: 574 111 68 162
Fragments arranged by size:
574 162 111 68
Enzymes that do cut:
AarI AccI AcuI AlwNI ApaLI ApoI AvaI BamHI
BanII Bme1580I BmrI BpmI BsaBI BsgI BsiHKAI Bsp1286I
BspMI BsrBI BsrFI BstXI BstYI Bsu36I CspCI Eco57MI
EcoNI EcoO109I EcoRV MmeI MslI MspA1I NaeI NgoMIV
PasI PflMI PfoI PpuMI PstI PvuII SfcI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AleI AlfI AloI ApaI AscI AseI AsiSI AvrII
BaeI BanI BbeI BbsI BbvCI BcgI BciVI BclI
BfrBI BglI BglII BlpI BmgBI BmtI BplI Bpu10I
BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI
BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrDI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstZ17I BtgI BtgZI
BtsI ClaI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI EcoRI FalI FseI FspI FspAI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MscI NarI NcoI NdeI NheI NotI NruI NsiI
NspI PacI PciI PmeI PmlI PpiI PshAI PsiI
PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StuI StyI SwaI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI